Tnni3 pSer150 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1/1000. Immunolabeling is greatly decreased with lambda -phosphatase treatment.
Reactivity	Human, Mouse, Primate, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser 150 of Mouse Troponin I, cardiac (cTnI).
Specificity	Specific for the ~25k cardiac troponin I protein phosphorylated at Ser 150.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol State: Aff - Purified State: Liquid purified Ig fraction
Purification	Affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	troponin I, cardiac 3
Database Link	Entrez Gene 21954 Mouse UniProt ID P48787
Background	Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser 23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser 23 and Ser 150 (Solis et al, 2011). Evidence su ggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser 150 over Ser 23 , and may play a role in regulating energy consumption through altering the p hosphorylation status of cTnI (Solis et al., 2011).
Synonyms	TNNI3, TNNC1
Reference Data

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