EPCAM Mouse Monoclonal Antibody [Clone ID: 323/A3]

Specifications

Product Data
Clone Name	323/A3
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry. Immunoprecipitation: 1-4 μg/ml per 100-500 μg of protein in 1 ml lysate. Western blot: 1-4 μg/ml. Positive Control: A-431, MCF-7, SW480 cell lines. Immunohistochemistry on Paraffin Sections : 1-10 μg/ml. Pretreatment: Tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0). Positive tissue: Breast carcinoma. Immunocytochemistry: 1-10 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human breast cancer MCF-7 cells
Specificity	This antibody recognizes CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.  Negative Species: Rat.
Formulation	PBS, pH 7.4 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	epithelial cell adhesion molecule
Database Link	Entrez Gene 4072 Human UniProt ID P16422
Background	CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.
Synonyms	Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18
Reference Data
Protein Families	ES Cell Differentiation/IPS, Transmembrane

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