AE2 (SLC4A2) Human shRNA Plasmid Kit (Locus ID 6522)

Specifications

Product Data
Product Name	AE2 (SLC4A2) Human shRNA Plasmid Kit (Locus ID 6522)
Locus ID	6522
UniProt ID	P04920
Synonyms	AE2; BND3L; EPB3L1; HKB3; NBND3
Vector	pRS
Format	Retroviral plasmids
Kit Components	SLC4A2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 6522). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	BC009386, BC010069, NM_001199692, NM_001199693, NM_001199694, NM_003040, NM_003040.1, NM_003040.2, NM_003040.3, NM_001199694.1, NM_001199694.2, NM_001199693.1, NM_001199692.1, NM_001199692.2, BC009386.2, BC010069.2, BC004893, BC009434, BC028601, BC128111, BM680391
Summary	This gene encodes a member of the anion exchanger family of membrane transport proteins. The encoded protein regulates intracellular pH, biliary bicarbonate secretion, and chloride uptake. Reduced expression of this gene may be associated with primary biliary cirrhosis (PBC) in human patients, while differential expression of this gene may be associated with malignant hepatocellular carcinoma, colon and gastric cancers. [provided by RefSeq, Nov 2016]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.