Inosine triphosphate pyrophosphatase (ITPA) Human shRNA Plasmid Kit (Locus ID 3704)

Specifications

Product Data
Product Name	Inosine triphosphate pyrophosphatase (ITPA) Human shRNA Plasmid Kit (Locus ID 3704)
Locus ID	3704
UniProt ID	Q9BY32
Synonyms	C20orf37; DEE35; dJ794I6.3; HLC14-06-P; ITPase; My049; NTPase
Vector	pRS
Format	Retroviral plasmids
Kit Components	ITPA - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 3704). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001267623, NM_033453, NM_181493, NR_052000, NR_052001, NR_052002, NM_001324236, NM_001324237, NM_001324238, NM_001324240, NM_001351739, NM_033453.1, NM_033453.2, NM_033453.3, NM_181493.2, NM_181493.3, NM_001267623.1, BC010138, BC010138.2, BM560812, BM703982, NM_033453.4, NM_181493.4, NM_001267623.2
Summary	This gene encodes an inosine triphosphate pyrophosphohydrolase. The encoded protein hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. This protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency which causes an accumulation of ITP in red blood cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.