2810474O19Rik Mouse shRNA Plasmid (Locus ID 67246)

Specifications

Product Data
Product Name	2810474O19Rik Mouse shRNA Plasmid (Locus ID 67246)
Locus ID	67246
UniProt ID	Q5DTW7
Synonyms	6720435I21Rik; AA536804; AU020969; GET; Kiaa1551
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	2810474O19Rik - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 67246). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001289661, NM_001289662, NM_026054, NM_026054.1, NM_026054.2, NM_026054.3, NM_001289661.1, NM_001289662.1, BC139435, BC023168, BC038922, BC055761, BC086679, BC089381, BC139436
Summary	Plays a role in the regulation of imprinted gene expression, regulates repressive epigenetic modifications associated with SETDB1. Required for the recruitment or accumulation of SETDB1 to the endogenous retroviruses (ERVs) and maintenance of repressive chromatin configuration, contributing to a subset of the SETDB1-dependent ERV silencing in embryonic stem cells.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.