Retinoblastoma binding protein 6 (RBBP6) Human shRNA Plasmid Kit (Locus ID 5930)

Specifications

Product Data
Product Name	Retinoblastoma binding protein 6 (RBBP6) Human shRNA Plasmid Kit (Locus ID 5930)
Locus ID	5930
UniProt ID	Q7Z6E9
Synonyms	MY038; P2P-R; PACT; RBQ-1; SNAMA
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	RBBP6 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 5930). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC029352, NM_006910, NM_018703, NM_032626, NM_018703.1, NM_018703.2, NM_018703.3, NM_032626.3, NM_032626.4, NM_032626.5, NM_006910.1, NM_006910.2, NM_006910.3, NM_006910.4, BC015318, BC029649, BC030964, BC051317, BC063524, BC073938, BC101139, BC101140, BC101141, BC101142, BC114353, BC114354, BC118667, BC139830, BC172357, BM314442, NM_006910.5, NM_018703.4
Summary	The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.