Phlpp2 (NM_001122594) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR219729 representing NM_001122594 Red=Cloning site Green=Tags(s) MGEVEPVPAGPLEPPEPPEAAAPRRPGGIRVLKRNMKHNGSRTCLNRRSRFGSRERDWLREDVKRGCVYL YGADTTTATTTTSSSSSSSSSSDLHLVLCTVETPASEICAGEGRESLYLQLHGDLVRRLEPSERPLQIVY DYLSRLGFEDPVRIQEEATNPDLSCMIRFYGEKPCQMDHLDRILLSGIYNVRKGKTQLHKWAERLVVLCG TCLIVSSVKDCQTGKMHILPLVGGKIEEVKRRQHSLAFSSAGAQAQTYHVSFETLAEYQRWQRQASKVVS QRMSTVDLSCYSLEEVPEHLFYSQDITYLNLRHNFMQLERPGGLDTLHKFSQLKGLNLSHNKLGLFPVLL CEISTLTELSLSCNGFHDLPSQIGKLLNLQTLSLDGNGLTALPDELGNLRQLTSLGISFNDFRHIPEVLE KLTMLDKVAMAGNRLEVLNLGALTRMSQVKHVDLRMNHLKTVITENMEGNKHITHMDLRDNQLTDLDLSS LCSLEQLHCERNQLRELTLSGFSLRTLYASWNRLTAVNVYPVPSLLTSLELSQNLLECVPDWACEAKKLE ILDISHNLLTEVPMRILSSLSLRKLMVGHNHIHVLPALVEHIPLEVLDIQHNTLSRLPDTLFSKALNLRY LNASANSLESLPSACAGEESLSVLQLLYLTSNLLTDQCIPVLVGHPHLRVLHLANNQLQTFPASKLNKLE QLEELNLSGNKLTAIPTTIANCKRLHTLVAHANNISIFPEILQLPQIQFVDLSCNDLTEILIPEALPATL QDLDLTGNTNLVLEHKTLDMFSHITALKIDQKPLPATDSAVTSTFWSHGLAEMAGQRNKLCVSALAMDNF AEGVGAVYGMFDGDRNEELPRLLQCTMADVLLEEVQHSTNDTVFMTNTFLVSHRKLGMAGQKLGSSALLC YIRPDTADPTSSFSLTVANVGMCQAVLCRGGKPVPLSKVFSLEHDPEEAQRVKDQKAIITEDNKVNGVTC CTRLLGCTYLYPWILPKPHIASTPLTIQDELLILGNKALWEHLSYLEAVNAVRHVQDPLAAAKKLCTLAQ SYGCQDNVGAMVVYLNIGEEGCTCEMNGLTLPGPVGFASTAALKDTPKPTTPSSSSGIASEFSSEMSTSE VSSEVGSTASDEHNTVGLEASLLPRPERRCSLHPASSAGVFQRQPSCATFSSNQSDNGLDSDDDQPVEGV ITNGSRVEVEVDIHCCRGREPESSPPLPKNSSNACSEERARGAGFGIRRQNSVNSGILLPANRDKMELQK SPSTSCLYGKKLSNGSIVPLEDSLNLIEVATEAPKRKTGYFAAPTQLEPEDQFVVPRDLEEEVKEQMKQH QEGRPEPEPRGEERTEPLEEFDTAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	150.1 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_001116066
Locus ID	244650
UniProt ID	Q8BXA7, J3QM82
Refseq Size	7854
Cytogenetics	8 D3
Refseq ORF	4065
Synonyms	AI481772; C130044A18Rik; Phlppl
Summary	Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (By similarity).[UniProtKB/Swiss-Prot Function]

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