PIN4 (1-156, His-tag) Human Protein
CAT#: AR09938PU-N
PIN4 (1-156, His-tag) human recombinant protein, 0.1 mg
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CNY 9,910.00
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规格
Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLERFSVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK
|
Tag | His-tag |
Predicted MW | 18.8 kDa |
Concentration | lot specific |
Purity | >85% |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glyceril 1mM, DTT, 0.1 mM PMSF |
Preparation | Liquid purified protein |
Applications | Protocol: Activity Assay 1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20 nM chymotrypsin. 2. Add 10 ul of recombinant PIN4 protein with 1 ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20 ul pre-chilled 5 mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460 mM LiCl to a concentration of 3 mM) 5. Record the increase in A405 nm for 30 minutes at 25°C. |
Protein Description | Recombinant human PIN4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Bioactivity | Specific: > 300 nmoles/min/mg, defined as the amount of enzyme that cleaves 1 umole of suc-AAPF-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin. |
Reference Data | |
RefSeq | NP_001164218 |
Locus ID | 5303 |
UniProt ID | Q9Y237 |
Cytogenetics | Xq13.1 |
Synonyms | EPVH; PAR14; PAR17 |
Summary | This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq, Dec 2009] |
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