FOLR3 (NM_000804) Human Over-expression Lysate
CAT#: LY400281
Transient overexpression lysate of folate receptor 3 (gamma) (FOLR3)
View other "FOLR3" proteins (2)
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CNY 2,950.00
CNY 600.00
CNY 300.00
CNY 1,430.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Description | Transient overexpression lysate of folate receptor 3 (gamma) (FOLR3) |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC212963 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Predicted MW | 27.7 kDa |
Components | The lysate is shipped with dry ice. Upon receiving, store the sample at -20°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_000795 |
Locus ID | 2352 |
Cytogenetics | 11q13.4 |
Protein Families | WB |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
TP701079 | Purified recombinant protein of Human folate receptor 3 (gamma) (FOLR3), Gln23-End, with C-terminal His tag, secretory expressed in HEK293 cells, 50ug |
CNY 7,106.00 |
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TP760307 | Recombinant protein of human folate receptor 3 (gamma) (FOLR3), with N-terminal HIS tag, expressed in E.Coli, 50ug |
CNY 2,040.00 |