PHLPP2 (NM_001289003) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PHLPPL; PPM3B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001289003, the custom clone sequence may differ by one or more nucleotides ATGAAACGCAATGGGAGCAGAAATTGTTTGAATAGGAGAAGTAGGTTTGGTTCTCGAGAAAGAGACTGGC TAAGAGAAGATGTAAAGAGAGGCTGTGTTTACCTTTATGGAGCAGACACTACCACTGCCACTACAACCAC CACCACCTCCTCTTCCTCTTCCTCCTCCTCCTCTTCCTCTGACTTACATCTCGTCCTTTGCACTGTAGAG ACACCAGCATCAGAAATATGTGCTGGAGAGGGAAGAGAAAGTCTTTATTTACAGCTTCATGGAGACCTGG TCAGGAGACTGGAACCTACTGAACGACCTCTTCAGATCGTTTATGATTACTTATCCAGGCTGGGATTTGA TGATCCTGTGCGCATACAGGAGGAGGCTACAAATCCTGACCTCGGCTGTATGATTCGATTTTATGGTGAA AAACCATGCCACATGGATCGTTTGGATCGAATCCTATTGTCTGGCATCTATAATGTACGCAAGGGAAAGA CCCAGCTGCATAAGTGGGCTGAGCGCCTAGTTGTCCTCTGTGGTACCTGCCTTATCGTTTCCTCAGTGAA GGATTGTCAAACTGGAAAGATGCACATTTTGCCTCTGGTTGGTGGAAAGATAGAAGAAGTGAAGCGACGG CAATACTCCCTTGCTTTCAGCTCAGCAGGAGCCCAAGCTCAGACCTATCATGTCAGCTTCGAGACTTTGG CCGAGTACCAGCGATGGCAACGGCAAGCATCCAAGGTGGTGTCCCAGCGAATCAGTACCGTGGATCTCTC GTGTTACAGCCTCGAGGAGGTTCCTGAGCATCTCTTCTATAGTCAAGATATTACCTACCTCAACTTGCGA CACAACTTCATGCAGTTAGAAAGACCCGGAGGCCTCGATACACTCTACAAATTTTCTCAACTGAAGGGCC TGAACTTGTCCCATAATAAACTTGGGTTGTTTCCTATATTGTTATGCGAGATCTCTACCCTGACTGAGCT CAACCTTTCCTGTAATGGATTTCATGACCTACCAAGTCAAATTGGCAATCTGCTAAATCTTCAAACCCTC TGTCTTGATGGCAACTTTCTGACTACTTTACCTGAAGAATTGGGAAATCTACAACAGCTTTCCTCCTTGG GAATTTCCTTCAACAACTTTAGTCAAATTCCTGAGGTTTATGAGAAACTCACTATGTTAGATAGAGTGGT TATGGCAGGAAATTGCCTGGAAGTCCTGAACTTAGGGGTGCTGAATAGGATGAACCATATCAAGCATGTG GATTTAAGGATGAACCATTTGAAAACCATGGTTATTGAAAATCTGGAGGGAAATAAACACATCACCCACG TGGATCTGCGGGACAACCGACTGACTGACTTGGATCTTAGCTCCTTATGCAGCTTGGAACAGCTGCACTG TGGGCGGAATCAGCTGAGGGAGCTAACACTCAGTGGCTTTTCCCTTCGGACCCTCTATGCCAGTTCCAAC AGGCTGACAGCAGTGAACGTCTATCCAGTACCCAGCCTGCTCACTTTCTTGGATCTCTCCCGAAACCTGC TAGAGTGTGTCCCTGACTGGGCCTGTGAAGCAAAGAAGATAGAAGTATTAGATGTGAGCTATAATCTTCT CACAGAGGTTCCCGTGAGAATTCTGAGTAGCTTGAGTCTTAGAAAACTGATGCTGGGACACAATCATGTG CAAAACCTTCCAACACTGGTAGAGCACATCCCCCTCGAGGTGCTGGATCTTCAGCATAATGCACTCACGA GGCTGCCAGACACCCTCTTCTCCAAGGCCTTAAACAAACTAAATAAATTGGAGCAATTGGAGGAACTGAA CCTAAGTGGCAACAAGCTTAAAACCATTCCCACAACCATAGCAAACTGTAAAAGGCTGCACACCCTTGTT GCACACTCCAACAACATCAGCATTTTCCCAGAAATACTGCAGTTGCCTCAGATCCAGTTTGTAGACCTAA GTTGCAACGACTTGACAGAAATCCTGATTCCAGAGGCTTTGCCTGCTACATTACAAGACCTTGACCTGAC TGGAAATACAAATCTGGTTCTGGAACACAAGACACTGGACATATTTAGCCATATCACAACCCTGAAAATT GATCAGAAACCTTTGCCAACCACAGATTCTACAGTTACGTCAACCTTCTGGAGCCATGGACTGGCTGAGA TGGCAGGGCAGAGAAATAAGCTGTGTGTCTCAGCACTTGCTATGGATAGCTTTGCAGAGGGGGTGGGAGC TGTGTATGGCATGTTTGATGGAGACCGAAATGAGGAGCTCCCGCGCCTGCTGCAGTGTACGATGGCAGAT GTGCTTTTAGAAGAGGTACAGCAGTCAACTAATGACACAGTTTTCATGGCTAACACCTTCTTGGTATCTC ACAGGAAATTAGGAATGGCTGGCCAGAAGTTGGGCTCCTCCGCTCTCCTGTGCTACATCCGCCCTGACAC TGCCGATCCAGCAAGTAGCTTTAGCTTGACTGTAGCCAATGTTGGCACGTGCCAAGCAGTCCTGTGCCGA GGTGGGAAGCCAGTGCCCCTCTCTAAAGTCTTCAGCCTGGAGCAGGACCCAGAGGAGGCTCAAAGGGTGA AGGACCAAAAAGCCATCATCACAGAGGACAACAAAGTGAATGGGGTAACCTGCTGTACCCGGATGCTGGG CTGTACATACCTCTACCCTTGGATCCTCCCCAAGCCCCACATATCTTCCACTCCGCTGACCATTCAAGAT GAGTTGCTGATTCTGGGAAACAAAGCATTGTGGGAACACTTGTCCTACACAGAAGCTGTCAATGCTGTAC GTCACGTACAAGACCCATTAGCAGCTGCTAAGAAGCTGTGCACATTAGCGCAGAGCTATGGCTGTCAGGA CAATGTAGGGGCGATGGTAGTTTATTTGAATATTGGTGAGGAAGGCTGCACTTGTGAAATGAATGGGCTC ACCCTCCCAGGTCCTGTGGGATTTGCTTCAACCACCACTATCAAGGATGCCCCTAAGCCAGCCACTCCAT CCTCTAGCAGTGGGATTGCCTCTGAGTTCAGCAGTGAGATGTCCACCTCAGAGGTGAGCAGTGAAGTGGG GTCCACTGCTTCTGATGAGCATAATGCTGGGGGCCTGGACACTGCCTTGCTTCCGAGGCCAGAGCGGCGC TGCAGCCTCCACCCAACACCCACCTCTGGGCTGTTTCAGCGCCAGCCTTCTTCTGCTACCTTCTCCAGTA ACCAGTCTGACAACGGCCTGGACAGTGATGATGACCAGCCCGTTGAGGGGGTCATAACCAATGGCAGCAA GGTAGAGGTGGAAGTAGACATCCACTGCTGCAGGGGGAGGGATCTGGAGAACTCACCCCCTCTCATAGAG AGTTCTCCTACCCTGTGTTCTGAGGAACATGCTAGAGGGTCGTGTTTTGGGATCCGAAGACAGAACAGTG TGAATAGTGGCATGCTCCTGCCAATGAGCAAGGACAGGATGGAGTTACAGAAGTCTCCCTCCACCTCCTG CCTCTATGGGAAGAAACTCTCCAATGGCTCTATTGTGCCCCTAGAGGACAGCCTGAACCTCATTGAAGTG GCCACAGAAGTGCCCAAGAGGAAAACTGGCTATTTTGCTGCCCCCACTCAGATGGAACCAGAGGACCAGT TTGTTGTGCCTCATGACCTGGAAGAAGAAGTGAAGGAACAAATGAAACAGCACCAGGACAGCCGGCTCGA GCCTGAGCCCCATGAAGAGGATCGGACCGAGCCCCCGGAGGAGTTCGACACAGCACTATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001289003
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289003.1, NP_001275932.1
RefSeq Size	8463 bp
RefSeq ORF	3771 bp
Locus ID	23035
UniProt ID	Q6ZVD8
Gene Summary	Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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