ROBO4 (NM_001301088) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AOVD3; ECSM4; MRB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301088, the custom clone sequence may differ by one or more nucleotides ATGGTGGCTGTGGTGGGTGAGCAGTTTACTCTGGAATGTGGGCCGCCCTGGGGCCACCCAGAGCCCACAG TCTCATGGTGGAAAGATGGGAAACCCCTGGCCCTCCAGCCCGGAAGGCACACAGTGTCCGGGGGGTCCCT GCTGATGGCAAGAGCAGAGAAGAGTGACGAAGGGACCTACATGTGTGTGGCCACCAACAGCGCAGGACAT AGGGAGAGCCGCGCAGCCCGGGTTTCCATCCAGGAGCCCCAGGACTACACGGAGCCTGTGGAGCTTCTGG CTGTGCGAATTCAGCTGGAAAATGTGACACTGCTGAACCCGGATCCTGCAGAGGGCCCCAAGCCTAGACC GGCGGTGTGGCTCAGCTGGAAGGTCAGTGGCCCTGCTGCGCCTGCCCAATCTTACACGGCCTTGTTCAGG ACCCAGACTGCCCCGGGAGGCCAGGGAGCTCCGTGGGCAGAGGAGCTGCTGGCCGGCTGGCAGAGCGCAG AGCTTGGAGGCCTCCACTGGGGCCAAGACTACGAGTTCAAAGTGAGACCATCCTCTGGCCGGGCTCGAGG CCCTGACAGCAACGTGCTGCTCCTGAGGCTGCCGGAAAAAGTGCCCAGTGCCCCACCTCAGGAAGTGACT CTAAAGCCTGGCAATGGCACTGTCTTTGTGAGCTGGGTCCCACCACCTGCTGAAAACCACAATGGCATCA TCCGTGGCTACCAGGTCTGGAGCCTGGGCAACACATCACTGCCACCAGCCAACTGGACTGTAGTTGGTGA GCAGACCCAGCTGGAAATCGCCACCCATATGCCAGGCTCCTACTGCGTGCAAGTGGCTGCAGTCACTGGT GCTGGAGCTGGGGAGCCCAGTAGACCTGTCTGCCTCCTTTTAGAGCAGGCCATGGAGCGAGCCACCCAAG AACCCAGTGAGCATGGTCCCTGGACCCTGGAGCAGCTGAGGGCTACCTTGAAGCGGCCTGAGGTCATTGC CACCTGCGGTGTTGCACTCTGGCTGCTGCTTCTGGGCACCGCCGTGTGTATCCACCGCCGGCGCCGAGCT AGGGTGCACCTGGGCCCAGGTCTGTACAGATATACCAGTGAGGATGCCATCCTAAAACACAGGATGGATC ACAGTGACTCCCAGTGGTTGGCAGACACTTGGCGTTCCACCTCTGGCTCTCGGGACCTGAGCAGCAGCAG CAGCCTCAGCAGTCGGCTGGGGGCGGATGCCCGGGACCCACTAGACTGTCGTCGCTCCTTGCTCTCCTGG GACTCCCGAAGCCCCGGCGTGCCCCTGCTTCCAGACACCAGCACTTTTTATGGCTCCCTCATCGCTGAGC TGCCCTCCAGTACCCCAGCCAGGCCAAGTCCCCAGGTCCCAGCTGTCAGGCGCCTCCCACCCCAGCTGGC CCAGCTCTCCAGCCCCTGTTCCAGCTCAGACAGCCTCTGCAGCCGCAGGGGACTCTCTTCTCCCCGCTTG TCTCTGGCCCCTGCAGAGGCTTGGAAGGCCAAAAAGAAGCAGGAGCTGCAGCATGCCAACAGTTCCCCAC TGCTCCGGGGCAGCCACTCCTTGGAGCTCCGGGCCTGTGAGTTAGGAAATAGAGGTTCCAAGAACCTTTC CCAAAGCCCAGGAGCTGTGCCCCAAGCTCTGGTTGCCTGGCGGGCCCTGGGACCGAAACTCCTCAGCTCC TCAAATGAGCTGGTTACTCGTCATCTCCCTCCAGCACCCCTCTTTCCTCATGAAACTCCCCCAACTCAGA GTCAACAGACCCAGCCTCCGGTGGCACCACAGGCTCCCTCCTCCATCCTGCTGCCAGCAGCCCCCATCCC CATCCTTAGCCCCTGCAGTCCCCCTAGCCCCCAGGCCTCTTCCCTCTCTGGCCCCAGCCCAGCTTCCAGT CGCCTGTCCAGCTCCTCACTGTCATCCCTGGGGGAGGATCAAGACAGCGTGCTGACCCCTGAGGAGGTAG CCCTGTGCTTGGAACTCAGTGAGGGTGAGGAGACTCCCAGGAACAGCGTCTCTCCCATGCCAAGGGCTCC TTCACCCCCCACCACCTATGGGTACATCAGCGTCCCAACAGCCTCAGAGTTCACGGACATGGGCAGGACT GGAGGAGGGGTGGGGCCCAAGGGGGGAGTCTTGCTGTGCCCACCTCGGCCCTGCCTCACCCCCACCCCCA GCGAGGGCTCCTTAGCCAATGGTTGGGGCTCAGCCTCTGAGGACAATGCCGCCAGCGCCAGAGCCAGCCT TGTCAGCTCCTCCGATGGCTCCTTCCTCGCTGATGCTCACTTTGCCCGGGCCCTGGCAGTGGCTGTGGAT AGCTTTGGTTTCGGTCTAGAGCCCAGGGAGGCAGACTGCGTCTTCATAGATGCCTCATCACCTCCCTCCC CACGGGATGAGATCTTCCTGACCCCCAACCTCTCCCTGCCCCTGTGGGAGTGGAGGCCAGACTGGTTGGA AGACATGGAGGTCAGCCACACCCAGCGGCTGGGAAGGGGGATGCCTCCCTGGCCCCCTGACTCTCAGATC TCTTCCCAGAGAAGTCAGCTCCACTGTCGTATGCCCAAGGCTGGTGCTTCTCCTGTAGATTACTCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001301088
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301088.1, NP_001288017.1
RefSeq Size	3653 bp
RefSeq ORF	2589 bp
Locus ID	54538
UniProt ID	Q8WZ75
Protein Families	Druggable Genome
Gene Summary	Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of the second exon and starts translation at a downstream AUG compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

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