TLK2 (NM_001284333) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HsHPK; MRD57; PKU-ALPHA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284333, the custom clone sequence may differ by one or more nucleotides ATGATGGAAGAATTGCATAGCCTGGACCCACGACGGCAGGAATTATTGGAGGCCAGGTTTACTGGAGTAG GTGTTAGTAAGGGACCACTTAATAGTGAGTCTTCCAACCAGAGCTTGTGCAGCGTCGGATCCTTGAGTGA TAAAGAAGTAGAGACTCCCGAGAAAAAGCAGAATGACCAGCGAAATCGGAAAAGAAAAGCTGAACCATAT GAAACTAGCCAAGGGAAAGGCACTCCTAGGGGACATAAAATTAGTGATTACTTTGAGTTTGCTGGGGGAA GCGCGCCAGGAACCAGCCCTGGCAGAAGTGTTCCACCAGTTGCACGATCCTCACCGCAACATTCCTTATC CAATCCCTTACCGCGACGAGTAGAACAGCCCCTCTATGGTTTAGATGGCAGTGCTGCAAAGGAGGCAACG GAGGAGCAGTCTGCTCTGCCAACCCTCATGTCAGTGATGCTAGCAAAACCTCGGCTTGACACAGAGCAGC TGGCGCAAAGGGGAGCTGGCCTCTGCTTCACTTTTGTTTCAGCTCAGCAAAACAGTCCCTCATCTACGGG ATCTGGCAACACAGAGCATTCCTGCAGCTCCCAAAAACAGATCTCCATCCAGCACAGACAGACCCAGTCC GACCTCACAATAGAAAAAATATCTGCACTAGAAAACAGTAAGAATTCTGACTTAGAGAAGAAGGAGGGAA GAATAGATGATTTATTAAGAGCCAACTGTGATTTGAGACGGCAGATTGATGAACAGCAAAAGATGCTAGA GAAATACAAGGAACGATTAAATAGATGTGTGACAATGAGCAAGAAACTCCTTATAGAAAAGTCAAAACAA GAGAAGATGGCGTGTAGAGATAAGAGCATGCAAGACCGCTTGAGACTGGGCCACTTTACTACTGTCCGAC ACGGAGCCTCATTTACTGAACAGTGGACAGATGGTTATGCTTTTCAGAATCTTATCAAGCAACAGGAAAG GATAAATTCACAGAGGGAAGAGATAGAAAGACAACGGAAAATGTTAGCAAAGCGGAAACCTCCTGCCATG GGTCAGGCCCCTCCTGCAACCAATGAGCAGAAACAGCGGAAAAGCAAGACCAATGGAGCTGAAAATGAAA CGCCCTCTTCTGGGAATACAGAGCTAAAGGATACAGCCCCAGCCTTAGGAGCCCACAGTTTACTTAGGTT AACGTTAGCAGAATACCATGAACAAGAAGAAATCTTCAAACTCAGATTAGGTCATCTTAAAAAGGAGGAA GCAGAGATCCAGGCAGAGCTGGAGAGACTAGAAAGGGTTAGAAATCTACATATCAGGGAACTAAAAAGGA TACATAATGAAGATAATTCACAATTTAAAGATCATCCAACGCTAAATGACAGATATTTGTTGTTACATCT TTTGGGTAGAGGAGGTTTCAGTGAAGTTTACAAGGCATTTGATCTAACAGAGCAAAGATACGTAGCTGTG AAAATTCACCAGTTAAATAAAAACTGGAGAGATGAGAAAAAGGAGAATTACCACAAGCATGCATGTAGGG AATACCGGATTCATAAAGAGCTGGATCATCCCAGAATAGTTAAGCTGTATGATTACTTTTCACTGGATAC TGACTCGTTTTGTACAGTATTAGAATACTGTGAGGGAAATGATCTGGACTTCTACCTGAAACAGCACAAA TTAATGTCGGAGAAAGAGGCCCGGTCCATTATCATGCAGATTGTGAATGCTTTAAAGTACTTAAATGAAA TAAAACCTCCCATCATACACTATGACCTCAAACCAGGTAATATTCTTTTAGTAAATGGTACAGCGTGTGG AGAGATAAAAATTACAGATTTTGGTCTTTCGAAGATCATGGATGATGATAGCTACAATTCAGTGGATGGC ATGGAGCTAACATCACAAGGTGCTGGTACTTATTGGTATTTACCACCAGAGTGTTTTGTGGTTGGGAAAG AACCACCAAAGATCTCAAATAAAGTTGATGTGTGGTCGGTGGGTGTGATCTTCTATCAGTGTCTTTATGG AAGGAAGCCTTTTGGCCATAACCAGTCTCAGCAAGACATCCTACAAGAGAATACGATTCTTAAAGCTACT GAAGTGCAGTTCCCGCCAAAGCCAGTAGTAACACCTGAAGCAAAGGCGTTTATTCGACGATGCTTGGCCT ACCGAAAGGAGGACCGCATTGATGTCCAGCAGCTGGCCTGTGATCCCTACTTGTTGCCTCACATCCGAAA GTCAGTCTCTACAAGTAGCCCTGCTGGAGCTGCTATTGCATCAACCTCTGGGGCGTCCAATAACAGTTCT TCTAATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001284333
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284333.1, NP_001271262.1
RefSeq Size	5518 bp
RefSeq ORF	2319 bp
Locus ID	11011
UniProt ID	Q86UE8
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (C) represents the longest transcript and encodes the longest isoform (C). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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