TMEM24 (C2CD2L) (NM_001290474) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DLNB23; TMEM24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290474, the custom clone sequence may differ by one or more nucleotides ATGGATCCGGGCTGGGGGCAGCGGGACGTGGGCTGGGCGGCCTTGCTGATCCTCTTCGCCGCCTCGCTGC TCACGGTGTTCGCCTGGCTGCTGCAATATGCCCGGGGCTTGTGGCTGGCGCGGGCCCGCGGGGACCGGGG CCCGGGACCCGCCTTAGCCGGGGAACCCGCGGGTTCCCTGCGGGAGCTGGGCGTGTGGCGCTCGCTGCTG CGGCTGCGGGCGACTCGGGCTGGCGCCGCCGAGGAGCCAGGAGTCCGGGGCCTCCTGGCGTCACTCTTCG CCTTCAAGTCTTTCCGGGAGAACTGGCAGCGGGCTTGGGTGCGAGCGCTGAACGAGCAGGCCTGCAGAAA CGGGAGCTCCATCCAAATCGCCTTTGAGGAGGTGCCCCAACTCCCACCCAGAGCCAGCATCAGTCATGTG ACCTGCGTAGACCAATCTGAGCATACCATGGTGCTGCGTTGCCAGCTCTCTGCTGAGGAGGTGCGGTTCC CAGTCTCTGTGACCCAGCAGTCCCCCGCTGCCGTCTCCATGGAGACCTACCACGTCACTCTGACACTGCC ACCAACACAGTTGGAAGTCAACCTGGAGGAAATCCCTGGTGAGGGGCTGCTCATATCCTGGGCCTTCACT GATCGCCCAGATCTCAGCCTAACGGTGCTTCCCAAGCTTCAGGCCAGGGAGAGAGGTGAAGAACAAGTGG AGCTCTCCACAATTGAGGAACTGATCAAGGATGCCATAGTCAGCACCCAGCCAGCCATGATGGTCAACCT CAGGGCTTGCTCTGCCCCAGGAGGCCTGGTACCCAGTGAGAAGCCACCCATGATGCCCCAGGCTCAGCCA GCCATCCCCAGACCTAACCGGTTATTCCTACGGCAGCTTCGGGCATCTCACTTGGGAAATGAGCTGGAAG GCACCGAGGAACTGTGCTGTGTAGCTGAACTCGACAACCCCATGCAGCAGAAGTGGACCAAGCCCGCGAG GGCTGGATCCGAGGTGGAGTGGACAGAAGACCTGGCACTGGATCTGGGCCCCCAGAGCCGGGAGCTGACC CTCAAAGTGCTGAGGAGCAGCAGCTGTGGAGACACCGAACTCCTAGGCCAGGCCACACTGCCTGTGGGCT CCCCCTCCAGACCACTGTCTCGAAGACAGTTGTGCCCACTCACCCCAGGGCCAGGGAAAGCCCTGGGACC AGCAGCCACCATGGCAGTGGAGCTTCACTATGAGGAGGGCTCTCCCCGGAACCTGGGTACTCCCACCTCC TCCACTCCACGCCCCAGCATCACACCTACCAAGAAGATTGAGCTTGACCGGACCATCATGCCCGATGGCA CCATTGTCACCACAGTCACCACTGTCCAGTCCCGGCCCCGTATAGACGGCAAATTAGACTCCCCCTCCCG CTCCCCGTCCAAGGTGGAGGTGACCGAGAAGACGACAACTGTGCTGAGTGAGAGCAGTGGCCCCAGCAAT ACCTCCCATAGCAGCAGCCGGGACAGCCACCTTTCCAACGGCTTGGACCCTGTAGCAGAGACAGCGATTC GCCAGCTGACAGAGCCCAGTGGGCGGGTGGCCAAGAAGACACCCACCAAGCGCAGCACTCTCATCATCTC TGGTGTTTCCAAGGTGCCCATTGCTCAGGACGAGTTGGCGCTATCCCTGGGCTATGCGGCATCCCTGGAA GCCTCAGTGCAGGATGATGCAGGGACCAGCGGAGGCCCCTCTTCACCTCCCTCAGACCCACCAGCCATGT CTCCAGGACCGCTAGATGCCCTCTCTAGTCCCACAAGTGTCCAGGAAGCAGACGAGACAACCCGTTCGGA TATTTCTGAGAGGCCATCTGTGGATGATATTGAGTCGGAAACGGGGTCCACTGGTGCCCTGGAGACCCGC AGCCTCAAGGATCACAAAGTGAGTTTCCTGCGCAGCGGCACTAAGCTCATCTTCCGCCGGAGGCCTAGGC AGAAGGAAGCTGGCCTGAGCCAATCACACGATGACCTCTCCAACGCAACGGCCACGCCCAGTGTCCGAAA GAAGGCCGGCAGCTTTTCTCGCCGCCTTATCAAGCGCTTTTCCTTCAAATCCAAACCCAAGGCCAATGGT AACCCCAGCCCCCAGCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001290474
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290474.1, NP_001277403.1
RefSeq Size	3399 bp
RefSeq ORF	2121 bp
Locus ID	9854
UniProt ID	O14523
Protein Families	Transmembrane
Gene Summary	Lipid-binding protein that transports phosphatidylinositol, the precursor of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), from its site of synthesis in the endoplasmic reticulum to the cell membrane (PubMed:28209843). It thereby maintains the pool of cell membrane phosphoinositides, which are degraded during phospholipase C (PLC) signaling (PubMed:28209843). Plays a key role in the coordination of Ca(2+) and phosphoinositide signaling: localizes to sites of contact between the endoplasmic reticulum and the cell membrane, where it tethers the two bilayers (PubMed:28209843). In response to elevation of cytosolic Ca(2+), it is phosphorylated at its C-terminus and dissociates from the cell membrane, abolishing phosphatidylinositol transport to the cell membrane (PubMed:28209843). Positively regulates insulin secretion in response to glucose: phosphatidylinositol transfer to the cell membrane allows replenishment of PI(4,5)P2 pools and calcium channel opening, priming a new population of insulin granules (PubMed:28209843).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1.

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