ALKBH8 (NM_001301010) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ABH8; MRT71; TRM9; TRMT9; TRMT9A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337162 representing NM_001301010. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTGCTATGGACAGCAACCATCAAAGTAATTACAAACTCAGTAAAACTGAGAAGAAGTTCTTAAGG AAACAGATTAAAGCCAAGCATACTTTGCTGAGACATGAAGGCATTGAGACAGTATCCTATGCCACTCAG AGCCTGGTTGTTGCCAATGGTGGTTTGGGTAATGGTGTGAGTCGGAACCAGCTGCTCCCGGTTTTAGAG AAATGTGGACTGGTGGATGCTCTCTTAATGCCACCTAACAAGCCGTACTCATTTGCAAGATACAGAACT ACAGAAGAATCTAAGAGAGCCTATGTTACCCTCAATGGAAAAGAAGTAGTGGATGATTTAGGACAAAAG ATCACTCTGTATTTGAATTTTGTGGAAAAAGTGCAGTGGAAGGAGTTGAGGCCTCAAGCCTTACCACCA GGACTCATGGTAGTAGAAGAAATAATTTCTTCTGAGGAGGAGAAAATGCTTTTGGAAAGTGTTGATTGG ACAGAAGATACAGACAATCAAAACTCTCAAAAATCCTTAAAACACAGAAGAGTAAAGCATTTTGGTTAT GAGTTCCACTATGAGAACAACAATGTAGATAAAGATAAGCCATTATCTGGGGGTCTTCCTGACATTTGT GAAAGCTTTTTGGAGAAATGGTTGAGGAAAGGTTACATTAAACATAAACCTGATCAAATGACCATAAAT CAGTATGAACCTGGGCAAGGAATTCCCGCTCATATTGATACACATTCCGCTTTTGAGGATGAGATCGTT TCTCTCAGTTTGGGGTCAGAGATTGTCATGGATTTTAAGCACCCAGATGGCATTGCAGTGCCAGTTATG TTGCCTCGTCGGAGTTTGCTGGTGATGACAGGAGAATCTAGATACCTTTGGACCCATGGAATCACGTGC AGAAAATTTGATACTGTTCAAGCATCTGAGAGTCTTAAAAGTGGAATTATCACCAGTGATGTTGGAGAC TTAACTTTAAGCAAGAGGGGACTACGAACATCATTTACATTTAGGAAAGTGAGGCAAACACCTTGTAAC TGTAGTTACCCGTTGGTCTGTGATAGCCAGAGGAAAGAGACTCCCCCCTCATTTCCAGAGAGTGATAAA GAAGCCTCACGGCTGGAGCAAGAGTACGTCCATCAGGTTTATGAAGAGATTGCTGGGCACTTCAGCAGC ACAAGACATACCCCTTGGCCGCACATTGTGGAGTTTTTGAAGGCTTTGCCAAGTGGTTCAATAGTGGCT GATATTGGATGTGGTAATGGAAAGTATCTTGGCATCAATAAGGAGTTATATATGATTGGTTGTGATCGT AGCCAAAACCTTGTGGACATTTGTAGAGAGAGGCAATTTCAGGCTTTTGTCTGTGATGCATTGGCAGTA CCAGTCCGCAGTGGGTCTTGTGATGCCTGCATCTCCATTGCTGTTATTCATCATTTTGCAACAGCAGAG CGTAGAGTGGCAGCTCTCCAAGAAATTGTTCGACTCCTGAGACCAGGTGGGAAGGCACTCATTTATGTC TGGGCAATGGAACAAGAATATAATAAGCAGAAGTCCAAGTATCTTAGAGGAAACAGAAATAGCCAAGGA AAGAAAGAGGAGATGAACAGTGATACCTCAGTGCAGAGGTCACTTGTGGAGCAAATGCGTGACATGGGC AGTCGAGACTCGGCATCTTCTGTCCCCCGCATTAATGACTCTCAGGAAGGAGGATGTAATTCAAGGCAA GTTTCTAATTCCAAGCTGCCTGTTCATGTTAACAGGACTTCTTTTTATTCTCAAGATGTACTGGTTCCC TGGCACCTTAAGGGAAATCCTGATAAAGGCAAACCTGTTGAGCCATTTGGTCCCATAGGATCCCAGGAC CCAAGTCCTGTGTTTCATCGTTACTACCATGTGTTCCGTGAGGGAGAACTGGAAGGTGCCTGCAGGACT GTGAGTGATGTCAGAATTCTGCAAAGCTACTACGATCAAGGAAACTGGTGTGTGATTCTTCAAAAGGCC TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301010
Insert Size	2004 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301010.1
RefSeq Size	4056 bp
RefSeq ORF	2004 bp
Locus ID	91801
UniProt ID	Q96BT7
Protein Families	Druggable Genome
MW	75.6 kDa
Gene Summary	Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.