IQCE (NM_001287502) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	1700028P05Rik; PAPA7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337066 representing NM_001287502. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCTCTGGGCGGCCGAGCGTCCCTGACCCCGCAGAAGCTGTGGCTGGGAACCGCAAAGCCAGGAAGT CTGACCCAGGCCCTGAACTCACCCCTCACCTGGGAGCATGCGTGGACTGGCGTCCCCGGCGGCACTCCT GACTGTCTGACAGACACCTTCAGAGTGAAGAGGCCACATCTCAGGCGCTCTGCCAGCAACGGTCATGTC CCTGGGACTCCTGTCTACAGAGAAAAAGAAGATATGTATGACGAGATTATTGAGTTAAAGAAGTCATTG CACGTGCAGAAGAGCGACGTGGACCTGATGAGAACGAAGCTCCGGCGCCTGGAGGAGGAAAACAGCAGG AAGGACCGGCAGATAGAGCAGCTCCTGGATCCCAGCCGCGGCACGGATTTTGTTCGGACTCTGGCAGAG AAAAGGCCCGATGCCAGTTGGGTCATTAACGGGCTGAAGCAGAGGATCCTGAAGCTGGAACAGCAGTGC AAGGAGAAGGACGGCACCATCAGCAAACTCCAGACCGATATGAAGACTACCAACCTGGAAGAGATGCGG ATCGCCATGGAGACATACTACGAGGAGGTGCATCGTCTCCAGACCCTCTTGGCAAGTTCTGAAACCACC GGAAAGAAGCCCCTGGGGGAGAAGAAGACGGGCGCCAAAAGGCAGAAGAAGATGGGCAGTGCCCTCCTG AGCTTGTCCCGGAGTGTCCAGGAGCTCACGGAAGAGAACCAGAGCCTGAAGGAGGACCTGGACCGCGTG CTGAGCACCTCCCCAACCATCTCCAAGACACAGGGTTATGTGGAGTGGAGCAAGCCCCGGCTGCTGAGG CGCATTGTGGAGCTGGAGAAGAAACTAAGTGTGATGGAGAGCTCAAAATCACACGCCGCAGAGCCAGTC AGATCACACCCGCCAGCCTGCCTTGCATCCAGCTCTGCGCTGCACAGACAGCCACGAGGGGACCGCAAC AAGGACCACGAGCGTCTCCGAGGGGCTGTGAGAGACCTGAAGGAAGAGCGGACCGCGCTGCAGGAGCAG CTGCTGCAGAGAGATTTGGAGGTGAAGCAGCTCCTGCAGGCGAAGGCCGACCTGGAGAAGGAGCTGGAG TGCGCGAGGGAGGGCGAGGAGGAGAGGAGAGAGCGAGAGGAGGTTTTGAGAGAGGAGATTCAGACACTT ACCAGCAAGCTCCAAGAATTGCAAGAAATGAAGAAAGAAGAGAAAGAGGATTGCCCGGAAGTTCCTCAT AAGGCCCAAGAGCTCCCAGCTCCCACTCCCAGCAGCAGGCACTGCGAGCAAGACTGGCCGCCGGATTCC AGCGAGGAGGGGCTCCCGCGGCCCCGCTCCCCCTGCTCTGATGGGAGAAGAGACGCCGCGGCCAGAGTC CTGCAGGCCCAGTGGAAGGTGTACAAGCACAAGAAAAAAAAGGCTGTTCTGGATGAGGCGGCTGTGGTG CTTCAGGCAGCTTTCAGGGGACATCTCACGCGGACAAAGCTCTTAGCAAGCAAAGCACATGGCTCAGAG CCACCCAGCGTGCCAGGCCTCCCAGACCAGAGCTCTCCTGTGCCCCGCGTTCCGAGCCCCATCGCCCAG GCCACGGGCAGCCCTGTGCAGGAGGAGGCCATCGTCATCATCCAGTCCGCTCTGCGGGCACACCTGGCC CGGGCCAGGCACAGTGCTACCGGTAAAAGAACCACCACCGCAGCTTCTACCAGGAGGAGATCGGCTTCA GCCACACACGGGGACGCCTCCTCCCCACCCTTCCTCGCAGCTCTTCCTGGTAATTTCATTCATTTGGAT TCTGGCACCAGGGCAGCTGGTCTGCTGAGGTCTCAGCCAAAGAGTGGCCACCTCCAGGAAGCCCGGGCT GTGTCGGGACGGAAGGGAGGAGTGTCCCATCTGGAGCTGCCTCTGAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287502
Insert Size	1914 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287502.1
RefSeq Size	2420 bp
RefSeq ORF	1914 bp
Locus ID	23288
MW	71.2 kDa
Gene Summary	Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling (By similarity). Required for proper limb morphogenesis (PubMed:28488682).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) lacks two alternate exons in the 5' coding region, initiates translation at a downstream in-frame start codon, and contains an alternate 3' terminal exon and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (6) is shorter at the N-terminus and has a distinct C-terminus, compared to isoform 1.

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