CCBL1 (KYAT1) (NM_001287390) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCBL1; GTK; KAT1; KATI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336574 representing NM_001287390. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTCAGGACTGCAGCAGCCATCTCTGTGCACCTGGTGGGGCCCCTTCAGGGAAGGAAGGCTGGAGCC CCACTCACTTGGTTCTTGCACCAGACTCAGGTGGCTGAGAAGAATGTTGGATCCCCAGCTCCACGGCCC ACCAGCCATCTGGGACTGGCAAACCCACAGGAACGGAAGGTTCCTCAGGGACCACAAAGCCCATGTGAG GTCCCTGCTGCTGAAGGGATTCTGAGGACAGGAGGAGCCAAATTACCTATCCAGGGTCACCCAGCCAAG CTCGCCATGGCCAAACAGCTGCAGGCCCGAAGGCTAGACGGGATCGACTACAACCCCTGGGTGGAGTTT GTGAAACTGGCCAGTGAGCATGACGTCGTGAACTTGGGCCAGGGCTTCCCGGATTTCCCACCACCAGAC TTTGCCGTGGAAGCCTTTCAGCACGCTGTCAGTGGAGACTTCATGCTTAACCAGTACACCAAGACATTT GGTTACCCACCACTGACGAAGATCCTGGCAAGTTTCTTTGGGGAGCTGCTGGGTCAGGAGATAGACCCG CTCAGGAATGTGCTGGTGACTGTTGGTGGCTATGGGGCCCTGTTCACAGCCTTCCAGGCCCTGGTGGAC GAAGGAGACGAGGTCATCATCATCGAACCCTTTTTTGACTGCTACGAGCCCATGACAATGATGGCAGGG GGTCGTCCTGTGTTTGTGTCCCTGAAGCCGGGTCCCATCCAGAATGGAGAACTGGGTTCCAGCAGCAAC TGGCAGCTGGACCCCATGGAGCTGGCCGGCAAATTCACATCACGCACCAAAGCCCTGGTCCTCAACACC CCCAACAACCCCCTGGGCAAGGTGTTCTCCAGGGAAGAGCTGGAGCTGGTGGCCAGCCTTTGCCAGCAG CATGACGTGGTGTGTATCACTGATGAAGTCTACCAGTGGATGGTCTACGACGGGCACCAGCACATCAGC ATTGCCAGCCTCCCTGGCATGTGGGAACGGACCCTGACCATCGGCAGCGCCGGCAAGACCTTCAGCGCC ACTGGCTGGAAGGTGGGCTGGGTCCTGGGTCCAGATCACATCATGAAGCACCTGCGGACCGTGCACCAG AACTCCGTCTTCCACTGCCCCACGCAGAGCCAGGCTGCAGTAGCCGAGAGCTTTGAACGGGAGCAGCTG CTCTTCCGCCAACCCAGCAGCTACTTTGTGCAGTTCCCGCAGGCCATGCAGCGCTGCCGTGACCACATG ATACGTAGCCTACAGTCAGTGGGCCTGAAGCCCATCATCCCTCAGGGCAGCTACTTCCTCATCACAGAC ATCTCAGACTTCAAGAGGAAGATGCCTGACTTGCCTGGAGCTGTGGATGAGCCCTATGACAGACGCTTC GTCAAGTGGATGATCAAGAACAAGGGCTTGGTGGCCATCCCTGTCTCCATCTTCTATAGTGTGCCACAT CAGAAGCACTTTGACCACTATATCCGCTTCTGTTTTGTGAAGGATGAAGCCACGCTCCAGGCCATGGAC GAGAAGCTGCGGAAGTGGAAGGTGGAACTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287390
Insert Size	1551 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287390.1
RefSeq Size	2487 bp
RefSeq ORF	1551 bp
Locus ID	883
MW	57.7 kDa
Gene Summary	This gene encodes a cytosolic enzyme that is responsible for the metabolism of cysteine conjugates of certain halogenated alkenes and alkanes. This metabolism can form reactive metabolites leading to nephrotoxicity and neurotoxicity. Increased levels of this enzyme have been linked to schizophrenia. Multiple transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) differs in the 5' UTR and CDS, and uses an alternate upstream translation start, compared to variant 1. The resulting protein (isoform c) has a longer N-terminus, compared to isoform a. Variants 4 and 12 both encode the same isoform (c). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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