MX1 (NM_001282920) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IFI-78K; IFI78; lncMX1-215; MX; MxA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336519 representing NM_001282920. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTTGTTTCCGAAGTGGACATCGCAAAAGCTGATCCAGCTGCTGCATCCCACCCTCTATTACTGAAT GGAGATGCTACTGTGGCCCAGAAAAATCCAGGCTCGGTGGCTGAGAACAACCTGTGCAGCCAGTATGAG GAGAAGGTGCGCCCCTGCATCGACCTCATTGACTCCCTGCGGGCTCTAGGTGTGGAGCAGGACCTGGCC CTGCCAGCCATCGCCGTCATCGGGGACCAGAGCTCGGGCAAGAGCTCCGTGTTGGAGGCACTGTCAGGA GTTGCCCTTCCCAGAGGCAGCGGGATCGTGACCAGATGCCCGCTGGTGCTGAAACTGAAGAAACTTGTG AACGAAGATAAGTGGAGAGGCAAGGTCAGTTACCAGGACTACGAGATTGAGATTTCGGATGCTTCAGAG GTAGAAAAGGAAATTAATAAAGCCCAGAATGCCATCGCCGGGGAAGGAATGGGAATCAGTCATGAGCTA ATCACCCTGGAGATCAGCTCCCGAGATGTCCCGGATCTGACTCTAATAGACCTTCCTGGCATAACCAGA GTGGCTGTGGGCAATCAGCCTGCTGACATTGGGTATAAGATCAAGACACTCATCAAGAAGTACATCCAG AGGCAGGAGACAATCAGCCTGGTGGTGGTCCCCAGTAATGTGGACATCGCCACCACAGAGGCTCTCAGC ATGGCCCAGGAGGTGGACCCCGAGGGAGACAGGACCATCGGAATCTTGACGAAGCCTGATCTGGTGGAC AAAGGAACTGAAGACAAGGTTGTGGACGTGGTGCGGAACCTCGTGTTCCACCTGAAGAAGGGTTACATG ATTGTCAAGTGCCGGGGCCAGCAGGAGATCCAGGACCAGCTGAGCCTGTCCGAAGCCCTGCAGAGAGAG AAGATCTTCTTTGAGAACCACCCATATTTCAGGGATCTGCTGGAGGAAGGAAAGGCCACGGTTCCCTGC CTGGCAGAAAAACTTACCAGCGAGCTCATCACACATATCTGTAAATCTCTGCCCCTGTTAGAAAATCAA ATCAAGGAGACTCACCAGAGAATAACAGAGGAGCTACAAAAGTATGGTGTCGACATACCGGAAGACGAA AATGAAAAAATGTTCTTCCTGATAGATAAAGTTAATGCCTTTAATCAGGACATCACTGCTCTCATGCAA GGAGAGGAAACTGTAGGGGAGGAAGACATTCGGCTGTTTACCAGACTCCGACACGAGTTCCACAAATGG AGTACAATAATTGAAAACAATTTTCAAGAAGGAGGCCAGCAAGCGCATCTCCAGCCACATCCCTTTGAT CATCCAGTTCTTCATGCTCCAGACGTACGGCCAGCAGCTTCAGAAGGCCATGCTGCAGCTCCTGCAGGA CAAGGACACCTACAGCTGGCTCCTGAAGGAGCGGAGCGACACCAGCGACAAGCGGAAGTTCCTGAAGGA GCGGCTTGCACGGCTGACGCAGGCTCGGCGCCGGCTTGCCCAGTTCCCCGGTTAACCACACTCTGTCCA GCCCCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282920
Insert Size	1527 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282920.1
RefSeq Size	1998 bp
RefSeq ORF	1527 bp
Locus ID	4599
UniProt ID	P20591
Protein Families	Druggable Genome
MW	55.7 kDa
Gene Summary	This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (4) differs in the 5' UTR and lacks three exons in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b, also known as varMxA) is shorter and has a distinct C-terminus, compared to isoform a. This variant is supported by data in PMID: 20603636.

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