TTC23 (NM_001288616) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HCC-8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336181 representing NM_001288616. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAAGAATCACAGGAAACCCACATATCCAACCACCTAGATGAAGTTGTTGCTGCTGTTAGCATCACT CATAGAAAGAAGTTCCAAAACAAGCTGCTTCAGACAGCACTATTCCAGCCTCCTCGAGAGAAACTCCAC CTCTGTGAAGAGAAAGCAAAGTCCTATTCCAACAGTCATGAGTACAAACAGGCCGTCCATGAGCTTGTG CGTTGCGTAGCACTGACAAGAATTTGCTATGGAGACTCACATTGGAAACTAGCAGAGGCACATGTTAAT CTGGCTCAAGGCTACCTCCAGCTGAAAGGACTGTCACTGCAAGCAAAACAACATGCAGAAAAAGCCAGA CAAATCCTCGCCAACTCCATTGTGCCTCCCTATAGTGAGAATACAGATGTTTTCAAGTTTTCCATTGAG CTTTTCCATACCATGGGCAGAGCTTTACTCTCCCTTCAAAAATTTAAGGAAGCTGCAGAGAATTTGACA AAAGCAGAGAGACTTTCAAAGGAGCTGCTACAATGTGGAAGAATTATAAAGGAAGAATGGATAGAAATT GAAGCACGGATCAGATTATCATTTGCACAGGTGTATCAAGGTCAGAAGAAGTCAAAAGAAGCTTTGTCC CACTATCAAGCAGCTTTGGAATATGTTGAGATCAGTAAAGGTGAAACAAGTCGTGAGTGTGTACCCATA TTGAGAGAATTAGCAGGTGTAGAGCAAGCCCTGGGACTCCACGATGTATCCATCAACCACTTCCTCCAG GCACATCTTATCATCCTGAGTAGAAGCCCCTCTCAAGTGGAGGCAGCAGACTCGGCACACATCGTCGCC CATGCTGCTGTCGCTTCAGGGAGACACGAGCACCATGATGTAGCTGAGCAGTATTTTCAAGAGAGCATG GCTCATCTTAAGGATTCTGAAGGGATGGGAAGAACCAAATTTCTTTCAATTCAAGATGAATTTTGCCAT TTTCTACAAATGACTGGACAAAAAGAGAGAGCAACCTCGATCCTGAGAGAGTCCCTGGAAGCCAAAGTG GAAGCATTTGGCGATTTCAGTCCCGAGGTGGCAGAGACATACCGGCTCCTGGGAGGAGCAGACCTGGCG CAGGGGAACCACAGTGGGGCCCGCAAGAAACTGAAGAAGTGTCTCCAGATCCAGACCCTCTTATATGGA CCGCAGGACAAAAGGACTCTGGCCACCCAGCAGGCCATGGGCATGCTGTCCACGGCCCCCAAGGTTGCT TCGAAGCCAAGGCAGGCATCAAAAGCCAAAGTGGCCTTCTGCACCAGCATCCCTCAGGACACCCTGCTG GGGAAGGCCCGGCCCGGCACAACAGCAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288616
Insert Size	1344 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288616.1
RefSeq Size	3324 bp
RefSeq ORF	1344 bp
Locus ID	64927
UniProt ID	Q5W5X9
MW	50 kDa
Gene Summary	Participates positively in the ciliary Hedgehog (Hh) signaling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (9) differs in the 5' UTR, compared to variant 8. Variants 8 and 9 encode the same protein.

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