HHLA2 (NM_001282558) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	B7-H5; B7-H7; B7H7; B7y
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335856 representing NM_001282558. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGGCACAGACAGCACTGTCTTTCTTCCTCATTCTCATAACATCTCTGAGTGGATCTCAAGGCATA TTCCCTTTGGCTTTCTTCATTTATGTTCCTATGAATGAACAAATCGTCATTGGAAGACTTGATGAAGAT ATAATTCTCCCTTCTTCATTTGAGAGGGGATCCGAAGTCGTAATACACTGGAAGTATCAAGATAGCTAT AAGGTTCACAGTTACTACAAAGGCAGTGACCATTTGGAAAGCCAAGATCCCAGATATGCAAACAGGACA TCCCTTTTCTATAATGAGATTCAAAATGGGAATGCGTCGCTATTTTTCAGAAGAGTAAGCCTTCTGGAC GAAGGAATTTACACCTGCTATGTAGGAACAGCAATTCAAGTGATTACAAACAAAGTGGTGCTAAAGGTG GGAGTTTTTCTCACACCCGTGATGAAGTATGAAAAGAGGAACACAAACAGCTTCTTAATATGCAGCGTG TTAAGTGTTTATCCTCGTCCAATTATCACGTGGAAAATGGACAACACACCTATCTCTGAAAACAACATG GAAGAAACAGGGTCTTTGGATTCTTTTTCTATTAACAGCCCACTGAATATTACAGGATCAAATTCATCT TATGAATGTACAATTGAAAATTCACTGCTGAAGCAAACATGGACAGGGCGCTGGACGATGAAAGATGGC CTTCATAAAATGCAAAGTGAACACGTTTCACTCTCATGTCAACCTGTAAATGATTATTTTTCACCAAAC CAAGACTTCAAAGTTACTTGGTCCAGAATGAAAAGTGGGACTTTCTCTGTCCTGGCTTACTATCTGAGC TCCTCACAAAATACAATTATCAATGAATCCCGATTCTCATGGAACAAAGAGCTGATAAACCAGAGTGAC TTCTCTATGAATTTGATGGATCTTAATCTTTCAGACAGTGGGGAATATTTATGCAATATTTCTTCGGAT GAATATACTTTACTTACCATCCACACAGTGCATGTAGAACCGAGCCAAGAAACAGCTTCCCATAACAAA GGCTTATGGATTTTGGTGCCCTCTGCGATTTTGGCAGCTTTTCTGCTGATTTGGAGCGTAAAATGTTGC AGAGAAAGATGTTGTGTCCCTCCTGGTGAGCGCTGTCCCAGTGCACCCGATAATGGCGAAGAAAATGTG CCTCTTTCAGGAAAAGTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282558
Insert Size	1194 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282558.1
RefSeq Size	2515 bp
RefSeq ORF	1194 bp
Locus ID	11148
UniProt ID	Q9UM44
Protein Families	Transmembrane
MW	45 kDa
Gene Summary	This gene encodes a protein ligand found on the surface of monocytes. The encoded protein is thought to regulate cell-mediated immunity by binding to a receptor on T lymphocytes and inhibiting the proliferation of these cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (4) represents the use of an alternate promoter, differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded protein (isoform b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.