MRP L9 (MRPL9) (NM_001300733) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	L9mt
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300733, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGCCCGTTGTCACGGCCCCGGGCAGAGCTCTGCTGCGGGCGGGCGCTGGACGGCTGCTTCGGG GAGGCGTCCAGGAGCTACTGCGGCCGCGACATGAAGGGAACGCCCCTGACCTGGCCTGCAACTTCAGCCT TTCTCAAAATCGGGGCACGGTCATCGTGGAGCGCTGGTGGAAGGTACCGCTGGCCGGGGAGGGCCGGAAG CCGCGCCTGCACCGGCGACATCGCGTCTATAAGCTGGTGGAGGACACGAAGCATCGGCCCAAAGAAAACC TGGAGCTCATCCTGACGCAGTCGGTGGAGAATGTTGGAGTCCGGGGTGACCTGGTCTCAGTGAAGAAATC TTTAGGCCGGAATCGACTCCTTCCTCAGGGACTGGCTGTATATGCATCCCCTGAAAACAAGAAGCTGTTT GAAGAGGAGAAATTGCTGAGACAAGAAGGAAAATTAGAGAAGATCCAGACCAAGGCAGGTGAGGCGCTTG GTGTTGTGGTTGCCCCACATACATTAAAGTTACCAGAAGAGCCTATCACACGGTGGGGCGAGTATTGGTG TGAGGTGACGGTAAATGGGCTTGATACTGTGAGAGTGCCTATGTCTGTCGTGAACTTTGAGAAGCCCAAG ACCAAAAGATATAAGTACTGGTTAGCCCAGCAAGCTGCCAAGGCTATGGCCCCCACCAGCCCCCAGATCT AA
Restriction Sites	SgfI-MluI
ACCN	NM_001300733
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300733.1, NP_001287662.1
RefSeq Size	1562 bp
RefSeq ORF	702 bp
Locus ID	65005
UniProt ID	Q9BYD2
Gene Summary	This is a nuclear gene encoding a protein component of the 39S subunit of the mitochondrial ribosome. Alternative splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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