PPPDE1 (DESI2) (NM_001297746) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C1orf121; CGI-146; DESI; DeSI-2; DESI1; FAM152A; PNAS-4; PPPDE1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC334076 representing NM_001297746. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGGCTAACCAGTTAGTGGTGCTCAACGTGTACGACATGTATTGGATGAACGAATATACCTCATCC ATTGGAATTGGAGTTTTTCATTCAGGAATTGAAGTCTATGGCAGAGCTGTTGTTTTAGGGAGCACGGAC TTCCTAGAAGATGATATAGAAAAAATTGTAGAAGAACTGGGAAAAGAATACAAAGGCAATGCTTATCAT TTAATGCATAAAAACTGCAATCATTTTTCTTCAGCTTTATCAGAGATTCTTTGTGGGAAAGAGATTCCT CGCTGGATCAATCGACTTGCCTACTTCAGCTCCTGTATACCCTTTCTACAGAGTTGCCTCCCGAAGGAG TGGCTCACGCCCGCAGCCCTGCAGTCTAGTGTCAGCCAAGAACTCCAGGATGAACTGGAGGAAGCAGAG GATGCTGCCGCATCCGCTTCCGTGGCAAGCACTGCAGCAGGCTCCAGACCCGGGCGCCACACTAAACTA TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297746
Insert Size	486 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297746.1
RefSeq Size	4010 bp
RefSeq ORF	486 bp
Locus ID	51029
UniProt ID	Q9BSY9
MW	17.8 kDa
Gene Summary	Has deubiquitinating activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Deubiquitinates 'Lys-48'-linked polyubiquitination of RPS7 leading to its stabilization (PubMed:28483520).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon and uses an alternate splice site, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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