ACCN2 (ASIC1) (NM_001256830) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACCN2; ASIC; BNaC2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332486 representing NM_001256830. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCATCCAGATCTTCTGCTCCATGTCATTCTCCTCTGGAGAGGAAGCCCCAGGGCCCTTGGGAGAT ATTTGGGGTCCCCACCACCATCAGCAGCAGCAGGACATCTCAGAATCGGAAGAGGAGGAAGAAGAGAAG GAAAAGGAGGCAGTGAGGAAGGAGGCCAGTGAGGGGCATTCACCCATGGACTTGGTGGCCTTTGCCAAC AGCTGCACCCTCCATGGCACCAACCACATTTTTGTGGAGGGGGGTCCAGGGCCAAGGCAGGTGCTGTGG GCGGTGGCCTTTGTCCTGGCACTGGGTGCCTTCCTGTGCCAGGTAGGGGACCGCGTTGCTTATTACCTC AGCTACCCACACGTGACCCTTCTAAACGAAGTGGCCACCACGGAGCTGGCCTTCCCGGCAGTCACCCTC TGCAACACTAATGCTGTGCGGCTGTCCCAGCTCAGCTACCCTGACTTGCTTTATTTGGCCCCCATGCTG GGACTGGATGAAAGTGATGACCCCGGGGTGCCCCTCGCTCCACCGGGCCCTGAGGCCTTCTCTGGGGAG CCCTTTAACCTGCACCGCTTCTACAATCGCTCCTGCCACCGGCTGGAGGACATGCTGCTCTATTGCTCC TACCAAGGGGGACCCTGCGGCCCTCACAACTTCTCAGTGGTCTTCACACGCTATGGAAAGTGCTACACG TTCAACTCGGGCCGAGATGGGCGGCCGCGGCTGAAGACCATGAAGGGTGGGACGGGCAATGGGCTGGAA ATCATGCTGGACATCCAGCAGGACGAGTACCTGCCTGTGTGGGGGGAGACTGACGAGACGTCCTTCGAA GCAGGCATCAAAGTGCAGATCCATAGTCAGGATGAACCTCCTTTCATCGACCAGCTGGGCTTTGGCGTG GCCCCAGGCTTCCAGACCTTTGTGGCCTGCCAGGAGCAGCGGCTCATCTACCTGCCCCCACCCTGGGGC ACCTGCAAAGCTGTTACCATGGACTCGGATTTGGATTTCTTCGACTCCTACAGCATCACTGCCTGCCGC ATCGACTGTGAGACGCGCTACCTGGTGGAGAACTGCAACTGCCGCATGGTGCACATGCCAGGGGATGCC CCATACTGTACTCCAGAGCAGTACAAGGAGTGTGCAGATCCTGCTCTGGACTTCCTGGTGGAGAAGGAC CAGGAGTACTGCGTGTGTGAAATGCCTTGCAACCTGACCCGCTATGGCAAAGAGCTGTCCATGGTCAAG ATCCCCAGCAAAGCCTCAGCCAAGTACCTGGCCAAGAAGTTCAACAAATCTGAGCAATACATAGGGGAG AACATCCTGGTGCTGGACATTTTCTTTGAAGTCCTCAACTATGAGACCATTGAACAGAAGAAGGCCTAT GAGATTGCAGGGCTCCTGGGTGACATCGGGGGCCAGATGGGGCTGTTCATCGGGGCCAGCATCCTCACG GTGCTGGAGCTCTTTGACTACGCCTACGAGGTCATTAAGCACAAGCTGTGCCGACGAGGAAAATGCCAG AAGGAGGCCAAAAGGAGCAGTGCGGACAAGGGCGTGGCCCTCAGCCTGGACGACGTCAAAAGACACAAC CCGTGCGAGAGCCTTCGGGGCCACCCTGCCGGGATGACATACGCTGCCAACATCCTACCTCACCATCCG GCCCGAGGCACGTTCGAGGACTTTACCTGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256830
Insert Size	1689 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256830.1
RefSeq Size	3207 bp
RefSeq ORF	1689 bp
Locus ID	41
UniProt ID	P78348
Protein Families	Druggable Genome, Ion Channels: Other
MW	62.7 kDa
Gene Summary	This gene encodes a member of the acid-sensing ion channel (ASIC) family of proteins, which are part of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Members of the ASIC family are sensitive to amiloride and function in neurotransmission. The encoded proteins function in learning, pain transduction, touch sensation, and development of memory and fear. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. This variant also uses an alternate, in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform a.

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