ATPBD4 (DPH6) (NM_001141972) Human Untagged Clone
CAT#: SC325546
ATPBD4 (untagged)-Human ATP binding domain 4 (ATPBD4), transcript variant 2
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ATPBD4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC325546 representing NM_001141972.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGGTCGCGGCTCTGATCAGTGGTGGGAAGGACAGCTGCTATAATATGATGCAGTGCATTGCTGCT GGGCATCAGATCGTTGCTTTAGCAAATCTAAGACCAGCTGAAAACCAAGTGGGGTCTGATGAACTGGAT AGCTACATGTATCAGACAGTGGGGCACCATGCCATTGACTTGTATGCAGAAGCAATGGCTCTTCCCCTC TATCGCCGAACCATAAGAGGAAGGAGCTTGGATACAAGACAAGTGTACACCAAATGTGAAGGTGATGAG GTTGAAGATCTCTATGAGCTTTTGAAACTTGTTAAGGGCATCACTAGAATGACCTTGCTTGCTGAATAT GATGCTCTGAATCTCCAAGATTTTCACATGCATTTGAAAGTGGGCAGCCAGGCGATTGTTTACAGGACT CCAAATGAACTGTGCACTCACAGCAAGTTTGATAAACACACATTTCCTCCTTTTATCAGTGAGATTGCA AAATGTGAAGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001141972 |
Insert Size | 498 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001141972.1 |
RefSeq Size | 2380 bp |
RefSeq ORF | 498 bp |
Locus ID | 89978 |
UniProt ID | Q7L8W6 |
MW | 18.7 kDa |
Gene Summary | Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor (EEF2) to diphthamide (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226708 | ATPBD4 (Myc-DDK-tagged)-Human ATP binding domain 4 (ATPBD4), transcript variant 2 |
CNY 1,200.00 |
|
RC226708L3 | Lenti ORF clone of Human ATP binding domain 4 (ATPBD4), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC226708L4 | Lenti ORF clone of Human ATP binding domain 4 (ATPBD4), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG226708 | ATPBD4 (tGFP-tagged) - Human ATP binding domain 4 (ATPBD4), transcript variant 2 |
CNY 4,370.00 |