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NEK2 (NM_002497) Human Untagged Clone
CAT＃: SC323443
NEK2 (untagged)-Kinase deficient mutant (K37M) of Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HsPK21; NEK2A; NLK1; PPP1R111; RP67 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323443 sequence for NM_002497 edited (data generated by NextGen Sequencing)
ATGCCTTCCCGGGCGGAGGACTATGAAGTGTTGTACACCATTGGCACAGGCTCCTACGGC CGCTGCCAGAAGATCCGGAGGAAGAGTGATGGCAAGATATTAGTTTGGATGGAACTTGAC TATGGCTCCATGACAGAAGCTGAGAAACAGATGCTTGTTTCTGAAGTGAATTTGCTTCGT GAACTGAAACATCCAAACATCGTTCGTTACTATGATCGGATTATTGACCGGACCAATACA ACACTGTACATTGTAATGGAATATTGTGAAGGAGGGGATCTGGCTAGTGTAATTACAAAG GGAACCAAGGAAAGGCAATACTTAGATGAAGAGTTTGTTCTTCGAGTGATGACTCAGTTG ACTCTGGCCCTGAAGGAATGCCACAGACGAAGTGATGGTGGTCATACCGTATTGCATCGG GATCTGAAACCAGCCAATGTTTTCCTGGATGGCAAGCAAAACGTCAAGCTTGGAGACTTT GGGCTAGCTAGAATATTAAACCACGACACGAGTTTTGCAAAAACATTTGTTGGCACACCT TATTACATGTCTCCTGAACAAATGAATCGCATGTCCTACAATGAGAAATCAGATATCTGG TCATTGGGCTGCTTGCTGTATGAGTTATGTGCATTAATGCCTCCATTTACAGCTTTTAGC CAGAAAGAACTCGCTGGGAAAATCAGAGAAGGCAAATTCAGGCGAATTCCATACCGTTAC TCTGATGAATTGAATGAAATTATTACGAGGATGTTAAACTTAAAGGATTACCATCGACCT TCTGTTGAAGAAATTCTTGAGAACCCTTTAATAGCAGATTTGGTTGCAGACGAGCAAAGA AGAAATCTTGAGAGAAGAGGGCGACAATTAGGAGAGCCAGAAAAATCGCAGGATTCCAGC CCTGTATTGAGTGAGCTGAAACTGAAGGAAATTCAGTTACAGGAGCGAGAGCGAGCTCTC AAAGCAAGAGAAGAAAGATTGGAGCAGAAAGAACAGGAGCTTTGTGTTCGTGAGAGACTA GCAGAGGACAAACTGGCTAGAGCAGAAAATCTGTTGAAGAACTACAGCTTGCTAAAGGAA CGGAAGTTCCTGTCTCTGGCAAGTAATCCAGAACTTCTTAATCTTCCATCCTCAGTAATT AAGAAGAAAGTTCATTTCAGTGGGGAAAGTAAAGAGAACATCATGAGGAGTGAGAATTCT GAGAGTCAGCTCACATCTAAGTCCAAGTGCAAGGACCTGAAGAAAAGGCTTCACGCTGCC CAGCTGCGTGCTCAAGCCCTGTCAGATATTGAGAAAAATTACCAACTGAAAAGCAGGCAG ATCCTGGGCATGCGCTAG Clone variation with respect to NM_002497.3 15 t=>g;110 a=>t;111 a=>g;504 t=>c;1269 g=>t;1317 a=>g >OriGene 5' read for mutant NM_002497 unedited
ACCGCCGTTCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCGGGTCAGTGCTG CTCGGGGGCTTCTCCATCCAGGTCCCTGGAGTTCCTGGTCCCTGGAGCTCCGCACTTGGCGGCGCAACCT GCGTGAGGCAGCGCGACTCTGGCGACTGGCCGGCCATGCCTTCCCGGGCGGAGGACTATGAAGTGTTGTA CACCATTTGGCACAGGCTCCTACGGCCGCTGCCAGAAAGATCCCGGAGGAAAGAGTGATGGGCAAGATAT TAGTTTGGATGGGAACTTTGACTATGGGCTCCCATGACAGAAAGCTGAAGAAAACAGAATGCCTTTGTTT CCTGAAAGTGATTTTGCCTCCTTGACCTGAACCATCCAAACCATCCGTTCCGTTACTATGATCCGGATTA TGGACCGGACAATATAAACACATGGTCCATTGAAAGGAAAAATTGAAAGGAGGGGATCTGGCTATGGAAT TTACAGGGGAACCAAGGAAAGGATTATTTATAAAAAGATTTTGTTTTTCGAGTATAACTCTTGACTCTGG GCCCGAGAGAGAGCCCCAGCGAAAATGTTAGTGGGCCACCCCTATTGCGCTGGTCTCTGACCCGCGACTG TGTTCTCTAGATGAGCACAACGTCTAGCGCGTGGACACTTGGCGCCTACTATATAACCCGAGCGAGTTTG TCAAACACTTGTGTCCACACATAATACTCTGTCAATGATACATATGCTCACTGAATCATATCTGAATGGC GCTCGGTGATATGGCTAAGCCATAGCTTGACAGAACTCTGGATAAGAGACGCAATTACGTTCTGAGTATG GATTCAG >SC323443 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCGGGTCAGTGCTGCTCGG GGGCTTCTCCATCCAGGTCCCTGGAGTTCCTGGTCCCTGGAGCTCCGCACTTGGCGGCGCAACCTGCGTG AGGCAGCGCGACTCTGGCGACTGGCCGGCCATGCCTTCCCGGGCG |
| Restriction Sites | Please inquire |
| ACCN | NM_002497 |
| Insert Size | 2210 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002497.2, NP_002488.1 |
| RefSeq Size | 2130 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 4751 |
| UniProt ID | P51955 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1, also known as NEK2A). The C-terminus (aa 399-445) of this isoform was shown to be responsible for its nucleolar localization (PMID:15161910). Isoforms 1 and 2 (which differ at the C-terminus) have also been reported to exhibit distinct pattern of expression during mitosis (PMID:11742531). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208133 | NEK2 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1 |
CNY 3,656.00 |
|
| RC208133L1 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208133L2 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RC208133L3 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208133L4 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG208133 | NEK2 (tGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1 |
CNY 5,256.00 |
|
| SC108482 | NEK2 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1 |
CNY 3,656.00 |
