Pepsin (PGA4) (NM_001079808) Human Untagged Clone

CAT#: SC315746

PGA4 (untagged)-Human pepsinogen 4, group I (pepsinogen A) (PGA4)



  "NM_001079808" in other vectors (4)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-PGA4 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC315746 representing NM_001079808.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAAGTGGCTGCTGCTGCTGGGTCTGGTGGCGCTCTCTGAGTGCATCATGTACAAGGTCCCCCTCATC
AGAAAGAAGTCCTTGAGGCGCACCCTGTCCGAGCGTGGCCTGCTGAAGGACTTCCTGAAGAAGCACAAC
CTCAACCCAGCCAGAAAGTACTTCCCCCAGTGGGAGGCTCCCACCCTGGTAGATGAACAGCCCCTGGAG
AACTACCTGGATATGGAGTACTTCGGCACTATCGGCATCGGAACTCCTGCCCAGGATTTCACCGTCGTC
TTTGACACCGGCTCCTCCAACCTGTGGGTGCCCTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCAC
AACCGCTTCAACCCTGAGGATTCTTCCACCTACCAGTCCACCAGCGAGACAGTCTCCATCACCTACGGC
ACCGGCAGCATGACAGGCATCCTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAG
ATCTTCGGCCTGAGCGAGACGGAACCTGGCTCCTTCCTGTATTATGCTCCCTTCGATGGCATCCTGGGG
CTGGCCTACCCCAGCATTTCCTCCTCCGGGGCCACACCCGTCTTTGACAACATCTGGAACCAGGGCCTG
GTTTCTCAGGACCTCTTCTCTGTCTACCTCAGCGCCGATGACCAGAGTGGCAGCGTGGTGATCTTTGGT
GGCATTGACTCTTCTTACTACACTGGAAGTCTGAACTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAG
ATCACCGTGGACAGCATCACCATGAACGGAGAGGCCATCGCCTGCGCTGAGGGCTGCCAGGCCATTGTT
GACACCGGCACCTCTCTGCTGACCGGCCCAACCAGCCCCATTGCCAACATCCAGAGCGACATCGGAGCC
AGCGAGAACTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCCTGCCCGACATCGTCTTC
ACCATCAATGGAGTCCAGTACCCCGTGCCACCCAGTGCCTACATCCTGCAGAGCGAGGGGAGCTGCATC
AGTGGCTTCCAGGGCATGAACCTCCCCACCGAATCTGGAGAGCTTTGGATCCTGGGTGATGTCTTCATC
CGCCAGTACTTTACCGTCTTCGACAGGGCAAACAACCAGGTCGGCCTGGCCCCCGTGGCTTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001079808
Insert Size 1167 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001079808.3
RefSeq Size 1383 bp
RefSeq ORF 1167 bp
Locus ID 643847
UniProt ID P00790
Protein Families Druggable Genome
MW 42 kDa
Gene Summary This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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