Supervillin (SVIL) (NM_021738) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MFM10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC315385 representing NM_021738. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAAAGAAAAGAAAGAATTGCCAGGCGCCTGGAAGGGATTGAAAATGACACTCAGCCCATCCTCTTG CAGAGCTGCACAGGATTGGTGACTCACCGCCTGCTGGAGGAAGACACCCCTCGATACATGAGAGCCAGC GACCCTGCCAGCCCCCACATCGGCCGATCAAATGAAGAGGAGGAAACTTCTGATTCTTCTCTAGAAAAG CAAACTCGATCCAAATACTGCACAGAAACCTCCGGTGTCCACGGTGACTCACCCTATGGTTCGGGTACC ATGGACACCCACAGTCTGGAGTCCAAAGCCGAAAGAATTGCAAGGTACAAAGCAGAAAGAAGGCGACAG CTGGCAGAGAAGTATGGGCTGACTCTGGATCCCGAGGCCGACTCCGAGTATTTATCCCGCTATACCAAG TCCAGGAAGGAGCCTGATGCTGTCGAGAAGCGGGGAGGAAAAAGTGACAAACAGGAAGAGTCAAGCAGA GATGCTAGTTCTCTGTACCCCGGGACCGAGACGATGGGGCTCAGGACCTGTGCCGGTGAATCCAAGGAC TATGCCCTCCATGTGGGTGACGGCTCTTCCGACCCGGAGGTGCTGCTGAACATAGAAAACCAAAGACGA GGTCAAGAGCTGAGTGCCACCCGGCAGGCCCATGACCTGTCCCCAGCAGCCGAGAGTTCCTCGACCTTC TCTTTCTCTGGGCGAGACTCCTCCTTCACTGAAGTGCCACGGTCCCCCAAGCACGCCCACAGCTCCTCC CTGCAGCAGGCAGCCTCCCGGAGCCCCTCCTTTGGTGACCCACAGCTATCCCCTGAGGCCCGACCCAGT ACAGGGAAACCCAAACATGAGTGGTTTCTCCAGAAAGATTCCGAAGGGGACACACCTTCACTTATCAAC TGGCCTTCCAGAGTTAAAGTTAGAGAAAAATTGGTGAAAGAGGAAAGTGCTCGAAACAGCCCTGAACTC GCCTCAGAGTCCGTAACTCAGAGGAGACACCAGCCAGCGCCAGTCCATTACGTGTCATTTCAGTCTGAG CACTCAGCCTTTGATAGGGTCCCCAGCAAGGCAGCAGGCTCTACACGACAGCCAATCCGTGGCTATGTC CAACCCGCAGATACCGGTCACACCGCCAAGCTAGTGACGCCAGAAACCCCAGAAAATGCATCTGAGTGT AGCTGGGTAGCATCAGCCACCCAGAATGTCCCCAAACCTCCCAGCTTGACGGTTCTAGAAGGTGACGGA AGGGATAGCCCAGTTCTCCATGTCTGCGAGTCAAAAGCAGAAGAAGAAGAAGGGGAAGGAGAAGGAGAA GAAAAAGAAGAAGATGTGTGCTTCACTGAAGCTCTCGAGCAAAGCAAGAAAACCCTACTGGCTTTGGAG GGTGATGGGCTAGTGAGAAGCCCAGAAGATCCCTCTAGAAATGAGGACTTTGGTAAGCCTGCTGTGAGC ACAGTCACCTTAGAGCATCAGAAGGAACTGGAAAACGTGGCACAACCCCCTCAAGCTCCGCACCAGCCC ACTGAGAGGACAGGCAGGAGCGAGATGGTTCTCTACATTCAAAGTGAGCCTGTGTCCCAAGACGCCAAA CCAACTGGTCACAACAGGGAAGCCTCGAAAAAGCGCAAGGTCCGTACCCGCTCTCTGTCAGATTTCACA GGCCCCCCTCAGCTCCAGGCCTTGAAGTATAAGGACCCAGCTTCCAGGAGAGAGCTGGAGCTGCCCAGC TCCAAGACCGAAGGGCCTTATGGGGAGATCAGCATGCTGGACACAAAAGTCTCTGTCGCCCAGCTCCGA AGTGCGTTCCTGGCATCTGCCAACGCCTGCAGGAGACCTGAACTCAAATCACGGGTGGAGAGGTCGGCT GAAGGACCTGGCTTGCCCACCGGTGTGGAACGGGAGAGAGGGTCCCGGAAACCAAGACGCTATTTTTCT CCTGGTGAAAGTAGAAAAACTTCCGAGAGATTTAGAACCCAACCTATAACTTCAGCAGAACGAAAGGAA TCGGATAGGTGCACTTCACATTCAGAAACGCCAACTGTCGATGATGAAGAAAAGGTGGATGAACGAGCC AAGCTGAGCGTCGCCGCCAAGAGGTTGCTTTTCAGGGAGATGGAAAAATCTTTTGATGAACAAAATGTT CCAAAGCGACGCTCAAGAAACACAGCTGTGGAGCAGAGGCTACGCCGTCTGCAGGACAGGTCCCTCACC CAGCCCATCACCACTGAAGAGGTGGTCATCGCAGCCACTGAACCTATCCCCGCTTCGTGTTCTGGGGGC ACCCACCCTGTAATGGCGAGACTTCCTAGCCCCACTGTAGCTAGGAGCGCTGTGCAGCCTGCCAGATTG CAGGCCTCTGCTCACCAAAAGGCCTTAGCCAAGGACCAGACAAATGAGGGCAAAGAGCTTGCTGAGCAA GGAGAACCTGATTCCTCCACTCTAAGCTTGGCCGAAAAGTTGGCCTTGTTTAACAAATTGTCCCAGCCA GTCTCAAAAGCGATTTCTACCCGGAACAGAATAGACACGAGACAGAGGAGAATGAACGCTCGCTATCAA ACTCAGCCAGTCACACTGGGAGAGGTGGAGCAGGTGCAGAGTGGAAAGCTCATTCCTTTCTCACCTGCC GTGAACACATCAGTGTCTACCGTAGCATCCACGGTTGCTCCAATGTATGCCGGAGATCTTCGCACAAAG CCACCTCTTGACCACAATGCAAGTGCCACTGACTATAAGTTTTCTTCTTCAATAGAAAATTCGGACTCT CCAGTTAGAAGCATTCTGAAATCGCAAGCTTGGCAGCCTTTGGTAGAGGGTAGCGAGAACAAGGGAATG TTGAGAGAATATGGAGAGACAGAAAGCAAGAGAGCTTTGACAGGTCGAGACAGTGGGATGGAGAAGTAT GGGTCCTTTGAGGAAGCAGAAGCATCCTACCCCATCCTGAACCGAGCCAGGGAAGGAGACAGCCATAAG GAATCTAAATATGCTGTTCCCAGAAGAGGAAGCCTGGAACGGGCGAACCCTCCCATCACCCACCTCGGG GATGAACCGAAGGAATTTTCCATGGCTAAAATGAATGCACAAGGAAACTTGGACTTGAGGGACAGGCTG CCCTTTGAAGAGAAGGTGGAGGTGGAGAATGTTATGAAAAGGAAGTTTTCACTAAGAGCGGCAGAGTTC GGGGAGCCCACTTCCGAGCAGACGGGGACAGCTGCTGGGAAAACTATTGCTCAAACCACAGCCCCCGTG TCCTGGAAGCCCCAGGATTCTTCGGAACAGCCACAGGAGAAGCTCTGCAAGAATCCATGTGCGATGTTT GCTGCTGGAGAGATCAAAACGCCGACAGGGGAGGGCCTTCTTGACTCACCCAGCAAAACCATGTCTATT AAAGAAAGATTGGCACTGTTGAAGAAAAGCGGGGAGGAAGATTGGAGAAACAGACTCAGCAGGAGGCAG GAGGGCGGCAAGGCGCCGGCCAGCAGCCTGCACACCCAGGAAGCAGGGCGGTCCCTCATCAAGAAGCGG GTCACAGAAAGTCGAGAGAGCCAAATGACGATTGAGGAGAGGAAGCAGCTCATCACTGTGAGAGAGGAG GCCTGGAAGACGAGAGGCAGAGGAGCGGCCAACGACTCGACCCAGTTCACTGTGGCTGGCAGGATGGTG AAGAAAGGTTTGGCGTCACCTACTGCCATAACCCCAGTAGCCTCACCCATTTGCGGTAAAACAAGAGGC ACCACACCCGTTTCCAAACCCCTGGAAGATATCGAAGCCAGACCAGATATGCAGTTAGAATCGGACCTG AAGTTGGACAGGCTGGAAACCTTTCTAAGAAGGCTGAATAACAAAGTTGGCGGGATGCACGAAACGGTG CTCACTGTCACCGGCAAATCTGTGAAGGAGGTGATGAAGCCAGATGATGATGAAACCTTTGCCAAATTT TACCGCAGCGTGGATTATAATATGCCAAGAAGTCCTGTGGAGATGGATGAGGACTTCGATGTCATTTTC GATCCTTATGCACCCAAATTGACGTCTTCCGTGGCCGAGCACAAGCGGGCAGTTAGGCCCAAGCGCCGG GTTCAGGCCTCCAAAAACCCCCTGAAAATGCTGGCGGCAAGAGAAGATCTCCTTCAGGAATACACTGAG CAGAGATTAAACGTTGCCTTCATGGAGTCAAAGCGGATGAAAGTAGAAAAGATGTCTTCCAACTCCAAC TTCTCAGAAGTCACCCTGGCGGGTTTAGCCAGTAAAGAAAACTTCAGCAACGTCAGCCTGCGGAGCGTC AACCTGACGGAACAGAACTCTAACAACAGCGCCGTGCCCTACAAGAGGCTGATGCTGTTGCAGATTAAA GGAAGAAGACATGTGCAGACCAGGCTGGTGGAACCTCGAGCTTCGGCGCTCAACAGTGGGGACTGCTTC CTCCTGCTCTCTCCCCACTGCTGCTTCCTGTGGGTAGGAGAGTTTGCAAACGTCATAGAAAAGGCGAAG GCCTCAGAACTTGCAACTTTAATTCAGACAAAGAGGGAACTTGGTTGTAGAGCTACTTATATCCAAACC ATTGAAGAAGGAATTAATACACACACTCATGCAGCCAAAGACTTCTGGAAGCTTCTGGGTGGCCAAACC AGTTACCAATCTGCTGGAGACCCAAAAGAAGATGAACTCTATGAAGCAGCCATAATAGAAACTAACTGC ATTTACCGTCTCATGGATGACAAACTTGTTCCTGATGACGACTACTGGGGGAAAATTCCGAAGTGCTCC CTTCTGCAACCCAAAGAGGTACTGGTGTTTGATTTTGGTAGTGAAGTTTACGTATGGCATGGGAAAGAA GTCACATTAGCACAACGAAAAATAGCATTTCAGCTGGCAAAGCACTTATGGAATGGAACCTTTGACTAT GAGAACTGTGACATCAATCCCCTGGATCCTGGAGAATGCAATCCGCTTATCCCCAGAAAAGGACAGGGG CGGCCCGACTGGGCGATATTTGGGAGACTTACTGAACACAATGAGACGATTTTGTTCAAAGAGAAGTTT CTGGATTGGACGGAACTGAAGAGATCGAATGAGAAGAACCCCGGGGAACTTGCCCAGCACAAGGAAGAC CCCAGGACTGATGTCAAGGCATACGATGTGACACGGATGGTGTCCATGCCCCAGACGACAGCAGGCACC ATCCTGGACGGAGTGAACGTCGGCCGTGGCTATGGCCTGGTGGAAGGACACGACAGGAGGCAGTTTGAG ATCACCAGCGTTTCCGTGGATGTCTGGCACATCCTGGAATTCGACTATAGCAGGCTCCCCAAACAAAGC ATCGGGCAGTTCCATGAGGGGGATGCCTATGTGGTCAAGTGGAAGTTCATGGTGAGCACGGCAGTGGGA AGTCGCCAGAAGGGAGAGCACTCGGTGAGGGCAGCCGGCAAAGAGAAGTGCGTCTACTTCTTCTGGCAA GGCCGGCACTCCACCGTGAGTGAGAAGGGCACGTCGGCGCTGATGACGGTGGAGCTGGACGAGGAAAGG GGGGCCCAGGTCCAGGTTCTCCAGGGAAAGGAGCCCCCCTGTTTCCTGCAGTGTTTCCAGGGGGGGATG GTGGTGCACTCGGGGAGGCGGGAAGAGGAAGAAGAAAATGTGCAAAGTGAGTGGCGGCTGTACTGCGTG CGTGGAGAGGTGCCCGTGGAAGGGAATTTGCTGGAAGTGGCCTGTCACTGTAGCAGCCTGAGGTCCAGA ACTTCCATGGTGGTGCTTAACGTCAACAAGGCCCTCATCTACCTGTGGCACGGATGCAAAGCCCAGGCC CACACGAAGGAGGTCGGAAGGACCGCTGCGAACAAGATCAAGGAACAATGTCCCCTGGAAGCAGGACTG CATAGTAGCAGCAAAGTCACAATACACGAGTGTGATGAAGGCTCCGAGCCACTCGGATTCTGGGATGCC TTAGGAAGGAGAGACAGGAAAGCCTACGATTGCATGCTTCAAGATCCTGGAAGTTTTAACTTCGCGCCC CGCCTGTTCATCCTCAGCAGCTCCTCTGGGGATTTTGCAGCCACAGAGTTTGTGTACCCTGCCCGAGCC CCCTCTGTGGTCAGTTCCATGCCCTTCCTGCAGGAAGATCTGTACAGCGCGCCCCAGCCAGCACTTTTC CTTGTTGACAATCACCACGAGGTGTACCTCTGGCAAGGCTGGTGGCCCATCGAGAACAAGATCACTGGT TCCGCCCGCATCCGCTGGGCCTCCGACCGGAAGAGTGCGATGGAGACTGTGCTCCAGTACTGCAAAGGA AAAAATCTCAAGAAACCAGCCCCCAAGTCTTACCTTATCCACGCTGGTCTGGAGCCCCTGACATTCACC AATATGTTTCCCAGCTGGGAGCACAGAGAGGACATCGCTGAGATCACAGAGATGGACACGGAAGTTTCC AATCAGATCACCCTCGTGGAAGACGTCTTAGCCAAGCTCTGTAAAACCATTTACCCGCTGGCCGACCTC CTGGCCAGGCCACTCCCGGAGGGGGTCGATCCTCTGAAGCTTGAGATCTATCTCACCGACGAAGACTTC GAGTTTGCACTAGACATGACGAGGGATGAATACAACGCCCTGCCCGCCTGGAAGCAGGTGAACCTGAAG AAAGCAAAAGGCCTGTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_021738
Insert Size	6645 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021738.2
RefSeq Size	8306 bp
RefSeq ORF	6645 bp
Locus ID	6840
UniProt ID	O95425
Domains	VHP, GEL, Gelsolin
Protein Families	Druggable Genome
MW	247.7 kDa
Gene Summary	This gene encodes a bipartite protein with distinct amino- and carboxy-terminal domains. The amino-terminus contains nuclear localization signals and the carboxy-terminus contains numerous consecutive sequences with extensive similarity to proteins in the gelsolin family of actin-binding proteins, which cap, nucleate, and/or sever actin filaments. The gene product is tightly associated with both actin filaments and plasma membranes, suggesting a role as a high-affinity link between the actin cytoskeleton and the membrane. The encoded protein appears to aid in both myosin II assembly during cell spreading and disassembly of focal adhesions. Several transcript variants encoding different isoforms of supervillin have been described. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (2) is alternatively spliced in the 5' portion of the gene, resulting in the longer, muscle-specific isoform (2) which has a different amino-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript that matched the reference genome was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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