RNF70 (PJA1) (AF262024) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PRAJA1; RNF70
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF262024, the custom clone sequence may differ by one or more nucleotides ATGGGTCAGGAATCTAGCAAGCCTGTATGGCCCAATCCAACAGGAGGGTATCAGTCCAAT ACAGGTAGGAGGTATGGAAGAAGGCATGCTTATGTCAGTTTCAGGCCACCCACGAGCCAG CGGGAAAGGATTGCCAGCCAGAGAAAGACGAACTCCGAAGTCCCAATGCACAGATCAGCC CCCAGTCAAACCACCAAGAGGAGCCGATCGCCATTTTCCACTACTCGTCGTAGTTGGGAC GACAGCGAGAGTTCGGGAACCAACCTGAATATTGATAATGAGGACTATTCCAGGTATCCG CCAAGAGAGTACAGAGCTTCGGGTAGCAGAAGAGGAATGGCCTATGGACATATTGACTCT TATGGGGCAGATGATAGTGAGGAGGAGGGGGCTGGGCCTGTTGAGCGACCGCCAGTGAGA GGGAAAACTGGCAAGTTTAAAGATGATAAGCTGTATGACCCAGAGAAAGGGGCAAGGTCT TTGGCTGGGCCACCTCCACATTTCTCTAGTTTTAGCCGTGATGTGAGAGAGGAGCGAGAC AAGTTAGACCCAGTCCCTGCAGCAAGATGCTCAGCTAGCAGAGCTGACTTCCTGCCACAA AGTAGTGTGGCCTCACAGTCGTCTTCTGAAGGCAAGCTGGCTACAAAAGGTGACAGCTCG GAGAGGGAGAGAAGGGAGCAAAATTTACCTGCACGTCCCAGCAGGGCTCCTGTGAGTATT TGTGGTGGTGGGGAAAACACCTCAAAGAGTGCAGAGGAACCTGTGGTCAGGCCCAAAATC AGAAACCTGGCAAGTCCAAACTGCGTGAAACCAAAAATTTTTTTTGATACTGATGATGAT GACGATATGCCACACAGTACTTCCAGGTGGAGGGATACCGCCAATGACAATGAGGGCCAC TCGGATGGCCTGGCAAGAAGAGGGAGAGGCGAGAGTTCAAGTGGCTATCCCGAGCCAAAG TACCCTGAAGACAAACGGGAAGCGAGGAGTGACCAAGTGAAACCAGAAAAGGTGCCGAGA CGACGACGCACCATGGCCGACCCTGACTTCTGGACGCACAGTGATGATTACTACAAATAC TGCGACGAAGACTCTGACAGTGACAAAGAGTGGATTGCTGCTCTGCGTCGGAAATATCGA AGCCGAGAGCAAACCCTGTCCTCCAGTGGCGAAAGCTGGGAGACTCTGCCGGGGAAAGAA GAGCGGGAACCTCCACAGGCTAAGGTGAGTGCCAGCACTGGCACCAGCCCTGGCCCCGGT GCTAGTGCCAGTGCCGGGGCTGGCGCCGGGGCCAGTGCTGGCAGCAATGGCAGCAATTAC CTTGAAGAAGTTCGAGAACCATCTCTTCAGGAAGAGCAGGCATCCCTGGAAGAAGGAGAA ATTCCTTGGCTCCAGTACCATGAGAATGACAGTAGCAGTGAGGGGGATAATGATTCTGGT CACGAGTTGATGCAACCTGGGGTATTCATGCTGGATGGAAACAACAACCTTGAAGATGAC TCCAGTGTGAGCGAAGACCTAGAAGTGGATTGGAGCCTCTTTGATGGATTTGCAGATGGG TTAGGAGTGGCTGAAGCCATTTCCTATGTGGACCCTCAGTTCCTCACCTACATGGCACTT GAAGAACGCCTGGCCCAGGCAATGGAAACTGCCCTTGCGCACTTGGAGTCTCTCGCAGTG GATGTAGAGGTGGCCAATCCACCAGCAAGCAAGGAGAGCATTGACGCTCTTCCCGAGATC CTGGTCACTGAAGATCATGGCGCAGTTGGTCAGGAGATGTGCTGCCCCATCTGCTGTAGC GAATATGTGAAGGGGGAGGTGGCAACTGAGCTGCCGTGCCACCACTATTTCCACAAGCCG TGTGTGTCCATCTGGCTTCAGAAGTCAGGCACCTGCCCCGTGTGCCGCTGCATGTTCCCT CCCCCACTC
Restriction Sites	Please inquire
ACCN	AF262024
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF262024.1, AAM53039.1
RefSeq Size	2700 bp
RefSeq ORF	2700 bp
Locus ID	64219
Domains	RING
Protein Families	Druggable Genome
Gene Summary	This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked cognitive disability disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

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