RIM1 (RIMS1) (NM_014989) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CORD7; RAB3IP2; RIM; RIM1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309143 representing NM_014989. Blue=Insert sequence Red=Cloning site Green=Tag(s) GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC ATGTCCTCGGCCGTGGGGCCCCGCGGTCCTCGCCCACCCACGGTGCCTCCCCCCATGCAAGAGCTGCCC GACCTGAGCCACCTGACCGAAGAGGAGAGGAACATTATCATGGCAGTGATGGACCGGCAGAAGGAAGAG GAGGAAAAAGAAGAAGCCATGCTCAAGTGTGTTGTCAGGGACATGGCGAAGCCTGCTGCCTGCAAAACA CCAAGAAATGCTGAAAACCAGCCCCACCAACCTTCACCGAGATTGCATCAACAGTTTGAAAGCTATAAG GAACAAGTGAGAAAAATAGGGGAAGAAGCGCGGCGTTACCAGGGCGAGCACAAAGACGATGCTCCGACT TGTGGAATCTGTCATAAAACAAAGTTTGCTGATGGGTGCGGTCATCTCTGCTCCTATTGTCGCACTAAG TTCTGTGCGCGCTGCGGAGGCCGCGTGTCTCTACGGTCAAACAACGAGGACAAAGTGGTTATGTGGGTA TGCAATTTATGTCGAAAGCAACAAGAAATCTTAACCAAATCTGGGGCATGGTTCTTTGGAAGTGGCCCT CAGCAGACAAGTCAGGATGGAACCCTGAGTGATACAGCTACAGGTGCTGGCTCTGAGGTACCAAGAGAA AAGAAAGCACGACTCCAAGAGCGATCGCGGTCTCAGACACCCCTAAGCACAGCAGCTGCCTCCTCCCAG GATGCTGCTCCTCCCAGCGCACCACCAGACAGGAGCAAAGGGGCTGAGCCCTCGCAGCAAGCCTTGGGG CCTGAACAGAAGCAGGCTTCATCCAGGTCTAGAAGTGAACCTCCTAGAGAGAGAAAGAAGACCCCAGGG CTTTCCGAGCAGAATGGCAAAGGAGCCCTGAAGAGCGAGCGGAAACGCGTGCCAAAGACCTCAGCGCAG CCCGTGGAGGGGGCCGTCGAAGAACGGGAGCGCAAAGAAAGGCGGGAAAGCCGAAGGCTTGAGAAAGGG CGATCACAGGATTACCCAGACACGCCGGAAAAACGGGATGAGGGCAAAGCGGCGGATGAGGAAAAGCAA AGAAAAGAGGAGGATTATCAGACCAGGTACCGCAGCGACCCGAACCTAGCTCGGTACCCGGTGAAACCG CCGCCTGAGGAGCAGCAGATGCGCATGCACGCCCGGGTGTCCCGCGCCAGGCACGAGCGGCGCCACAGC GACGTGGCGCTCCCGCGCACCGAGGCGGGCGCGGCGCTGCCGGAGGGCAAGGCCGGCAAACGCGCGCCG GCGGCAGCCAGGGCCTCGCCGCCGGACTCGCCGCGGGCTTACTCGGCTGAGAGAACTGCGGAGACCAGG GCGCCGGGCGCCAAGCAGCTAACGAACCACAGCCCGCCGGCGCCCAGACATGGGCCGGTTCCCGCAGAA GCCCCGGAGCTCAAAGCCCAGGAGCCCCTCAGGAAGCAGAGCCGCCTGGACCCCAGCTCGGCGGTCCTC ATGCGGAAGGCCAAGCGCGAGAAGGTGGAGACCATGCTGCGGAACGACTCTTTGAGCTCAGACCAGTCC GAGTCGGTGCGGCCGTCCCCGCCCAAGCCGCACCGGTCCAAGAGAGGCGGCAAGAAGCGGCAGATGTCG GTGAGCAGCTCTGAGGAGGAGGGCGTGTCGACGCCCGAGTACACCAGCTGCGAGGACGTGGAGCTGGAG AGCGAGAGCGTCAGCGAGAAAGGTGATTTGGATTATTACTGGTTGGATCCTGCCACGTGGCACAGCCGG GAGACATCACCTATTAGTTCGCATCCTGTAACGTGGCAACCATCTAAAGAGGGGGACCGATTAATTGGA CGTGTTATTCTTAACAAGAGAACAACCATGCCCAAAGACTCAGGTGCATTGCTGGGTCTGAAAGTTGTT GGAGGAAAAATGACTGACTTAGGACGACTTGGTGCTTTCATCACCAAAGTAAAGAAGGGTAGCCTAGCA GATGTAGTTGGACACCTAAGAGCAGGGGATGAAGTTCTAGAATGGAATGGTAAACCCCTGCCGGGAGCT ACAAATGAAGAAGTTTACAACATTATTTTAGAATCAAAATCAGAACCTCAAGTTGAAATTATTGTTTCA AGGCCTATTGGTGACATTCCCCGGATTCCTGAGAGCTCCCACCCTCCACTGGAGTCCAGTTCAAGTTCC TTTGAATCTCAGAAGATGGAAAGGCCTTCCATTTCTGTTATTTCTCCAACAAGTCCTGGAGCTCTAAAA GATGCCCCACAAGTCTTACCAGGGCAACTTTCTGTGAAGTTGTGGTATGATAAAGTGGGACACCAGCTG ATTGTAAATGTTCTGCAAGCAACAGATCTACCTGCTAGAGTAGATGGACGTCCTCGAAATCCCTATGTA AAAATGTATTTTCTTCCAGATAGAAGTGATAAAAGTAAAAGGAGGACCAAAACAGTAAAGAAAATACTA GAACCAAAATGGAATCAAACTTTTGTCTATTCACATGTACATCGTAGAGATTTTAGAGAACGAATGTTA GAAATAACTGTGTGGGACCAACCAAGAGTGCAAGAAGAAGAAAGTGAATTTCTTGGAGAGATCCTCATA GAATTGGAGACAGCGCTTTTAGATGATGAACCGCATTGGTATAAACTTCAGACACATGATGAGTCTTCA CTACCTCTGCCTCAGCCATCACCTTTCATGCCAAGGCGACATATTCATGGAGAAAGCTCTAGCAAAAAG CTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAGATTATGAGGTTGATGATGGTATTGGCGTA GTTCCTCCAGTAGGCTATAGGTCTAGTGCTAGAGAAAGTAAATCTACAACATTAACTGTGCCAGAACAG CAAAGAACAACTCATCACCGCTCACGTTCAGTATCTCCTCATCGCGGCAATGATCAGGGAAAGCCGCGT TCACGTTTACCAAATGTGCCATTACAGAGGAGTTTAGATGAAATTCATCCAACAAGAAGGTCACGTTCT CCAACCAGACACCATGATGCCTCCCGAAGTCCAGTTGATCATAGAACCAGAGATGTGGATAGTCAGTAT TTATCAGAACAAGACAGTGAGCTTCTTATGCTGCCCAGAGCAAAACGAGGACGAAGTGCAGAATGCCTA CATACTACCAGACATCTTGTTAGGCACTATAAAACATTACCTCCCAAGATGCCTTTATTACAGAGCAGT TCTCACTGGAATATTTACAGCTCAATTCTGCCTGCACATACTAAGACCAAATCAGTGACTAGACAGGAC ATTTCCCTTCATCATGAATGCTTTAACTCAACAGTATTGAGATTTACTGATGAAATACTGGTTAGTGAA CTGCAGCCCTTTCTTGACAGGGCTAGGAGTGCTAGTACCAACTGCTTGAGACCAGATACTAGTTTGCAT TCACCAGAACGAGAAAGGGGTAGATGGTCCCCCTCCCTAGATAGGAGACGACCTCCTAGTCCCAGGATT CAAATCCAGCATGCGTCTCCGGAGAATGACAGGCACTCCAGAAAGTCTGAAAGATCTAGCATCCAAAAA CAGACTAGGAAAGGCACTGCCTCTGATGCAGAAAGGGTTCTCCCAACATGTCTTTCTAGAAGGGGACAC GCAGCCCCAAGAGCAACTGATCAGCCAGTCATTAGGGGAAAACATCCTGCTCGCTCAAGGTCGAGTGAG CACTCTAGTATCAGAACACTGTGTTCTATGCACCACCTTGTCCCTGGAGGGTCGGCGCCACCTTCTCCG CTTCTGACAAGAATGCACCGACAGAGAAGTCCAACACAATCTCCTCCAGCAGACACATCGTTCAGCAGT CGCAGGGGAAGACAGCTCCCACAAGTGCCAGTGAGAAGCGGCAGTATAGAACAAGCAAGCTTAGTAGTG GAGGAGCGAACAAGACAGATGAAAATGAAAGTGCATCGATTTAAGCAGACAACAGGGTCTGGTTCTAGT CAAGAACTTGATCGCGAGCAATATTCCAAGTATAACATACATAAAGATCAGTACAGAAGCTGTGATAAC GTCTCTGCCAAATCATCAGATAGTGATGTCAGTGATGTTTCCGCCATTTCCCGAACCAGCAGTGCCTCA CGCCTCAGCAGCACAAGCTTTATGTCAGAGCAATCTGAGCGCCCCAGGGGTAGAATCAGTTCATTTACC CCCAAAATGCAAGGCAGACGGATGGGGACTTCAGGAAGATCCATCATGAAGAGCACCAGTGTCAGTGGA GAGATGTACACACTGGAGCATAATGACGGCAGCCAGTCAGACACAGCTGTGGGTACAGTTGGAGCAGGT GGAAAGAAACGGAGATCCAGCCTTAGTGCCAAAGTGGTTGCCATAGTGTCTCGAAGGAGTAGAAGCACA TCCCAGCTTAGTCAAACAGAGTCGGGCCACAAAAAGTTAAAAAGTACCATCCAGAGAAGCACAGAAACA GGCATGGCAGCTGAAATGAGAAAGATGGTAAGGCAGCCGAGCCGAGAGTCTACTGATGGCAGCATCAAC AGTTACAGCTCTGAGGGCAATTTAATATTTCCTGGAGTGCGACTGGGAGCTGACAGTCAATTCAGTGAT TTTCTTGATGGATTGGGACCAGCCCAGCTTGTTGGCCGCCAAACCCTTGCCACCCCTGCAATGGGTGAT ATACAAATAGGAATGGAGGACAAAAAGGGCCAATTAGAAGTGGAAGTCATTAGAGCACGAAGCCTCACA CAAAAGCCTGGTTCCAAATCTACACCTGCTCCATATGTCAAAGTATATCTTTTGGAAAATGGGGCCTGT ATAGCCAAGAAGAAGACAAGAATTGCACGAAAAACCCTTGATCCTTTGTATCAGCAGTCTCTGGTTTTT GATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGGAGACTATGGCAGAATGGACCACAAA TGCTTTATGGGTGTGGCTCAGATCTTGTTGGAAGAACTCGACCTGTCCAGCATGGTGATCGGATGGTAC AAATTGTTCCCACCGTCCTCACTGGTGGATCCCACACTCACTCCCCTCACCCGGCGGGCTTCCCAGTCA TCTCTGGAAAGTTCAACTGGGCCTCCCTGTATTCGATCATAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-RsrII
ACCN	NM_014989
Insert Size	5079 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_014989.5
RefSeq Size	7836 bp
RefSeq ORF	5079 bp
Locus ID	22999
UniProt ID	Q86UR5
Domains	C2, PDZ, RPH3A_effector
MW	189.1 kDa
Gene Summary	The protein encoded by this gene is a RAS gene superfamily member that regulates synaptic vesicle exocytosis. This gene also plays a role in the regulation of voltage-gated calcium channels during neurotransmitter and insulin release. Mutations have suggested a role cognition and have been identified as the cause of cone-rod dystrophy type 7. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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