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Pumilio 1 (PUM1) (NM_001020658) Human Untagged Clone
CAT＃: SC302206
PUM1 (untagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 1
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSPUM; PUMH; PUMH1; PUML1; SCA47 |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_001020658, the custom clone sequence may differ by one or more nucleotides
ATGAGCGTTGCATGTGTCTTGAAGAGAAAAGCAGTGCTTTGGCAGGACTCTTTCAGCCCC CACCTGAAACATCACCCTCAAGAACCAGCTAATCCCAACATGCCTGTTGTTTTGACATCT GGAACAGGGTCGCAAGCGCAGCCACAACCAGCTGCAAATCAGGCTCTTGCAGCTGGGACT CACTCCAGCCCTGTCCCAGGATCTATAGGAGTTGCAGGCCGTTCCCAGGACGACGCTATG GTGGACTACTTCTTTCAGAGGCAGCATGGTGAGCAGCTTGGGGGAGGAGGAAGTGGAGGA GGCGGCTATAATAATAGCAAACATCGATGGCCTACTGGGGATAACATTCATGCAGAACAT CAGGTGCGTTCCATGGATGAACTGAATCATGATTTTCAAGCACTTGCTCTGGAGGGAAGA GCGATGGGAGAGCAGCTCTTGCCAGGTAAAAAGTTTTGGGAAACAGATGAATCCAGCAAA GATGGACCAAAAGGAATATTCCTGGGTGATCAATGGCGAGACAGTGCCTGGGGAACATCA GATCATTCAGTTTCCCAGCCAATCATGGTGCAGAGAAGACCTGGTCAGAGTTTCCATGTG AACAGTGAGGTCAATTCTGTACTGTCCCCACGATCGGAGAGTGGGGGACTAGGCGTTAGC ATGGTGGAGTATGTGTTGAGCTCATCCCCGGGCGATTCCTGTCTAAGAAAAGGAGGATTT GGCCCAAGGGATGCAGACAGTGATGAAAACGACAAAGGTGAAAAGAAGAACAAGGGTACG TTTGATGGAGATAAGCTAGGAGATTTGAAGGAGGAGGGTGATGTGATGGACAAGACCAAT GGTTTACCAGTGCAGAATGGGATTGATGCAGACGTCAAAGATTTTAGCCGTACCCCTGGT AATTGCCAGAACTCTGCTAATGAAGTGGATCTTCTGGGTCCAAACCAGAATGGTTCTGAG GGCTTAGCCCAGCTGACCAGCACCAATGGTGCCAAGCCTGTGGAGGATTTCTCCAACATG GAGTCCCAGAGTGTCCCCTTGGACCCCATGGAACATGTGGGCATGGAGCCTCTTCAGTTT GATTATTCAGGCACGCAGGTACCTGTGGACTCAGCAGCAGCAACTGTGGGACTTTTTGAC TACAATTCTCAACAACAGCTGTTCCAAAGACCTAATGCGCTTGCTGTCCAGCAGTTGACA GCTGCTCAGCAGCAGCAGTATGCACTGGCAGCTGCTCATCAGCCGCACATCGGTTTAGCT CCCGCTGCGTTTGTCCCCAATCCATACATCATCAGCGCTGCTCCCCCAGGGACGGACCCC TACACAGCTGGATTGGCTGCAGCAGCGACACTAGGCCCAGCTGTGGTCCCTCACCAGTAT TATGGAGTTACTCCCTGGGGAGTCTACCCTGCCAGTCTTTTCCAGCAGCAAGCTGCCGCT GCCGCTGCAGCAACTAATTCAGCTAATCAACAGACCACCCCACAGGCTCAGCAAGGACAG CAGCAGGTTCTCCGTGGAGGAGCCAGCCAACGTCCTTTGACCCCAAACCAGAACCAGCAG GGACAGCAAACGGATCCCCTTGTGGCAGCTGCAGCAGTGAATTCTGCCCTTGCATTTGGA CAAGGTCTGGCAGCAGGCATGCCAGGTTATCCGGTGTTGGCTCCTGCTGCTTACTATGAC CAAACTGGTGCCCTTGTAGTGAATGCAGGCGCGAGAAATGGTCTTGGAGCTCCTGTTCGA CTTGTAGCTCCTGCCCCAGTCATCATTAGTTCCTCAGCTGCACAAGCAGCTGTTGCAGCA GCCGCAGCTTCAGCAAATGGAGCAGCTGGTGGTCTTGCTGGAACAACAAATGGACCATTT CGCCCTTTAGGAACACAGCAGCCTCAGCCCCAGCCCCAGCAGCAGCCCAATAACAACCTG GCATCCAGTTCTTTCTACGGCAACAACTCTCTGAACAGCAATTCACAGAGCAGCTCCCTC TTCTCCCAGGGCTCTGCCCAGCCTGCCAACACATCCTTGGGATTCGGAAGTAGCAGTTCT CTCGGCGCCACCCTGGGATCCGCCCTTGGAGGGTTTGGAACAGCAGTTGCAAACTCCAAC ACTGGCAGTGGCTCCCGCCGTGACTCCCTGACTGGCAGCAGTGACCTTTATAAGAGGACA TCGAGCAGCTTGACCCCCATTGGACACAGTTTTTATAACGGCCTTAGCTTTTCCTCCTCT CCTGGACCCGTGGGCATGCCTCTCCCTAGTCAGGGACCAGGACATTCACAGACACCACCT CCTTCCCTCTCTTCACATGGATCCTCTTCAAGCTTAAACCTGGGAGGACTCACGAATGGC AGTGGAAGATACATCTCTGCTGCTCCAGGCGCTGAAGCCAAGTACCGCAGTGCAAGCAGC GCCTCCAGCCTCTTCAGCCCGAGCAGCACTCTTTTCTCTTCCTCTCGTTTGCGATATGGA ATGTCTGATGTCATGCCTTCTGGCAGGAGCAGGCTTTTGGAAGATTTTCGAAACAACCGG TACCCCAATTTACAACTGCGGGAGATTGCTGGACATATAATGGAATTTTCCCAAGACCAG CATGGGTCCAGATTCATTCAGCTGAAACTGGAGCGTGCCACACCAGCTGAGCGCCAGCTT GTCTTCAATGAAATCCTCCAGGCTGCCTACCAACTCATGGTGGATGTGTTTGGTAATTAC GTCATTCAGAAGTTCTTTGAATTTGGCAGTCTTGAACAGAAGCTGGCTTTGGCAGAACGG ATTCGAGGCCACGTCCTGTCATTGGCACTACAGATGTATGGCTGCCGTGTTATCCAGAAA GCTCTTGAGTTTATTCCTTCAGACCAGCAGGTAATTAATGAGATGGTTCGGGAACTAGAT GGCCATGTCTTGAAGTGTGTGAAAGATCAGAATGGCAATCACGTGGTTCAGAAATGCATT GAATGTGTACAGCCCCAGTCTTTGCAATTTATCATCGATGCGTTTAAGGGACAGGTATTT GCCTTATCCACACATCCTTATGGCTGCCGAGTGATTCAGAGAATCCTGGAGCACTGTCTC CCTGACCAGACACTCCCTATTTTAGAGGAGCTTCACCAGCACACAGAGCAGCTTGTACAG GATCAATATGGAAATTATGTAATCCAACATGTACTGGAGCACGGTCGTCCTGAGGATAAA AGCAAAATTGTAGCAGAAATCCGAGGCAATGTACTTGTATTGAGTCAGCACAAATTTGCA AGCAATGTTGTGGAGAAGTGTGTTACTCACGCCTCACGTACGGAGCGCGCTGTGCTCATC GATGAGGTGTGCACCATGAACGACGGTCCCCACAGTGCCTTATACACCATGATGAAGGAC CAGTATGCCAACTACGTGGTCCAGAAGATGATTGACGTGGCGGAGCCAGGCCAGCGGAAG ATCGTCATGCATAAGATCCGGCCCCACATCGCAACTCTTCGTAAGTACACCTATGGCAAG CACATTCTGGCCAAGCTGGAGAAGTACTACATGAAGAACGGTGTTGACTTAGGGCCCATC TGTGGCCCCCCTAATGGTATCATCTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_001020658 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001020658.1, NP_001018494.1 |
| RefSeq Size | 5416 bp |
| RefSeq ORF | 3567 bp |
| Locus ID | 9698 |
| UniProt ID | Q14671 |
| Gene Summary | This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224913 | PUM1 (Myc-DDK-tagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 1 |
CNY 11,880.00 |
|
| RC224913L3 | Lenti-ORF clone of PUM1 (Myc-DDK-tagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 1 |
CNY 13,780.00 |
|
| RC224913L4 | Lenti-ORF clone of PUM1 (mGFP-tagged)-Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 1 |
CNY 13,780.00 |
|
| RG224913 | PUM1 (tGFP-tagged) - Human pumilio homolog 1 (Drosophila) (PUM1), transcript variant 1 |
CNY 10,696.00 |
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