CAMSAP1 (AL117634) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL117634, the custom clone sequence may differ by one or more nucleotides ACGCGTCCGCAGCTTGGCCCGCTCCATCAGCAAAGACAGTTTGGCATCCAACATTGTTAATCTGACCCCA CAGAACCAGCCACACCCCACGGCCACGAAGAGCCACGGGAAGAGCCTCCTGAGCAATGTCAGTATTGAGG ATGAGGAAGAGGAGCTTGTGGCTATTGTTAGAGCAGACGTGGTTCCCCAGCAGGCTGACCCGGAGTTCCC CAGGGCCTCACCCCGGGCCTTAGGCCTTACAGCAAATGCCCGGTCTCCCCAAGGACAGCTGGACACCTCG GAAAGTAAGCCTGACAGTTTTTTCTTGGAGCCTTTGATGCCAGCAGTTCTTAAGCCAGCGAAAGAGAAGC AGGTGATCACCAAGGAGGATGAACGGGGGGAAGGGAGACCGAGGAGCATCGTGTCTAGGAGGCCCAGCGA GGGCCCCCAGCCTTTGGTACGAAGGAAAATGACTGGCAGTCGCGACTTGAATAGGACTTTTACCCCGATT CCATGCTCAGAATTCCCCATGGGCATCGACCCCACCGAGACAGGACCACTGTCAGTGGAAACCGCAGGAG AGGTCTGTGGTGGGCCTCTGGCCTTGGCGGATTCGATCCGTTCCCCCAGGGACCATCCACGGATGGCTTC TTCCTTCATGTAGGCAGGGCCGATGAAGACACCGAGGGAAGGTTATATGTTAGTTGTTCAAAAAGCCCCA ACTCCCACGATTCAGAGCCGTGGACTCTCCTCAGGCAGGATTCTGACTCGGATGTGGTGGACATAGAGGA AGCCGAGCACGATTTCATGGGTGAGGCCCATCCTGTGGTTTTCAGCAGATACATTGGGGAGGAAGAGTCG GCCAAACTGCAGGAGGACATGAAGGTGAAGGAACATGAAGACAAAGATGACGCCAGC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL117634 unedited TCACTATAGGCGGCCGCGAATTCGCACGAGGAGAAGCAGGTGATCACCAAGGAGGATGAA CGGGGGGAAGGGAGACCGAGGAGCATCGTGTCTAGGAGGCCCAGCGAGGGCCCCCAGCCT TTGGTACGAAGGAAAATGACTGGCAGTCGCGACTTGAATAGGACTTTTACCCCGATTCCA TGCTCAGAATTCCCCATGGGCATCGACCCCACCGAGACAGGACCACTGTCAGTGGAAACC GCAGGAGAGGTCTGTGGTGGGCCTCTGGCCCTTGGCGGATTCGATCCGTTCCCCCAGGGA CCATCCACGGATGGCTTCTTCCTTCATGTAGGCAGGGCCGATGAAGACACCGAGGGAAGG TTANTATGTTAGTTGTTCAAAAAGCCCCAACTCCCACGATTCAGAGCCGTGGACTCTCCT CAGGCAGGATTCTGACTCGGATGTGGTGGACATAGAGGAAGCCGAGCACGATTTCATGGG TGAGGCCCATNNCTGTGGTTTTCAGCAGATACATTGGGGAAGGAAAGAGTCGGCCAAACT GCAGGAGGACATGAAGGGTGAAGGAACATGAAGACAAAGATGACGCCAGCGGCCGCTCGA GCCCCTGCCTGAGCACAGCCTCTCAATGGAGCAGTGTCTCATGGGCGAGTGGGGAGCGTG GAAAATGACCAGCTTTGCGGGAGAGGAAAGCTCAGAGACTCANCAGCTGTGAGACCAAGT NCAGCACAGCAGCTNCCANAAGACACGCAAGTGGCGTCTGAAAGCTGNCCACCCTTGACC AACCTGGAGCCAAAAAGGGGACCAAATCCGAGCACATGCAAGGAATCCGGCAGCCTCTGC ATTTTAGACTGATACGCTGCCTGCGCTGG
Restriction Sites	NotI-NotI
ACCN	AL117634
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL117634.1, CAB56024.2
RefSeq Size	897 bp
RefSeq ORF	897 bp
Locus ID	157922
Gene Summary	Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:19508979, PubMed:21834987, PubMed:24486153, PubMed:24706919, PubMed:24117850). Specifically recognizes growing microtubule minus-ends and stabilizes microtubules (PubMed:24486153, PubMed:24706919). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153, PubMed:24706919). In contrast to CAMSAP2 and CAMSAP3, tracks along the growing tips of minus-end microtubules without significantly affecting the polymerization rate: binds at the very tip of the microtubules minus-end and acts as a minus-end tracking protein (-TIP) that dissociates from microtubules after allowing tubulin incorporation (PubMed:24486153, PubMed:24706919). Through interaction with spectrin may regulate neurite outgrowth (PubMed:24117850).[UniProtKB/Swiss-Prot Function]

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