IGL (BC030983) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IGL
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC030983 edited GGGGTCTCAGGAGGCAGCACTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCC TCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCT CAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCCCCTGCACTGGAACCAGCAGTGATG TTGGTAATTATAACTATGTCTCCTGGTACCGACAACACCCAGGCAAAGCCCCCAAACTCA TGATTTATGATGTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT CTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATT ACTGCTGCTCATATGCAGGCACCTACACTTTCGGGGTGTTCGGCGGAGGGACCAAGCTGA CCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGG AGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCG TGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACAC CCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGC AGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGA AGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGA CTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACCCCAAGGCATCAAGCCCTT CTCCCTGCACTCAATAAACCCTCAATAAATATTCTCATTGTCAATCGAAAAAAAAAAAAA AAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC030983 unedited NGTCCACGATTTTGTNAATACGCACTCCACTCATAGGGGCGGGCCGCGCAATTCCGCCAT TACGGCCGGGGGGGGTCTCAGGAGGCAGCACTCTCGGGACGTCTCCACCATGGCCTGGGC TCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGAC TCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCCCCTGCACTGGAAC CAGCAGTGATGTTGGTAATTATAACTATGTCTCCTGGTACCGACAACACCCAGGCAAAGC CCCCAAACTCATGATTTATGATGTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTC TGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCGCTGGGCTCCANGCTGAGGATGA GGCTGATTATTACTGCTGCTCATATGCAGGCACCTACACTTTCGGGGTGTTCGGCGGAGG GACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC CTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTA CCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGGCGGGAGTGG AGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCC TGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCANGTCACGCATGAAGGGA GCACCGTGGAGAAAACAGTGGCCCCTACAGATGTTCATAGGTTCTCATCCCTCACCCCCA CCACGGGAGACTANAGCTGCAGGATCCCAGGGGAGGGGTCTCTCTCCCACCCCAGGCATC AAGCCCTTCTNCCTGGCCCTCATAAACCTCCATAAATATCTCCATTGTCATCGAAAAAAA AAA
Restriction Sites	Please inquire
ACCN	BC030983
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC030983.1, AAH30983.1
RefSeq Size	916 bp
Locus ID	3535
Gene Summary	Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments and three C segments are known to be incapable of encoding a protein and are considered pseudogenes. The locus also includes several non-immunoglobulin genes, many of which are pseudogenes or are predicted by automated computational analysis or homology to other species. [provided by RefSeq, Jul 2008]

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