RAN (BC016654) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARA24; Gsp1; guanosine triphosphatase Ran; member RAS oncogene family; OK/SW-cl.81; RAN, member RAS oncogene family; RanGTPase; ras-related nuclear protein; TC4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC016654 edited CGGGCGGCTGAGTAGGTGGGTGGTGCGGGCCCGGCCGGGCCGGGGCAGGAGACGGGCGTG GGGTCGGCGCTAGCCCCCGAGAACCCCCGTTTCATCCTCCGCTCTCATCCCCGTCCCGGT CCCAGTCCCGTTCCCATCCCTACACCTCCGTCCGCCGTTCCCCGGGCCCCGCCGCCCCGG ATGCCGGCCCCGCCCGCCGCCTTCCCGCTCCCAGGCCTGGCCGCCATGGCGCCGCGGGCG GGAGGCCTTTGTGGGGCGGGCACGTGGGGCGCTGGGGGCGCGGGAGCGGGGCCGCCATGG GCTGCGGGGCCGCGCGAGCGCTCGCCTCCGTCCTCTGCCTCCGCAGGAACGCCGCGATGG CTGCGCAGGGAGAGCCCCAGGTCCAGTTCAAACTTGTATTGGTTGGTGATGGTGGTACTG GAAAAACGACCTTCGTGAAACGTCATTTGACTGGTGAATTTGAGAAGAAGTATGTAGCCA CCTTGGGTGTTGAGGTTCATCCCCTAGTGTTCCACACCAACAGAGGACCTATTAAGTTCA ATGTATGGGACACAGCCGGCCAGGAGAAATTCGGTGGACTGAGAGATGGCTATTATATCC AAGCCCAGTGTGCCATCATAATGTTTGATGTAACATCGAGAGTTACTTACAAGAATGTGC CTAACTGGCATAGAGATCTGGTACGAGTGTGTGAAAACATCCCCATTGTGTTGTGTGGCA ACAAAGTGGATATTAAGGACAGGAAAGTGAAGGCGAAATCCATTGTCTTCCACCGAAAGA AGAATCTTCAGTACTACGACATTTCTGCCAAAAGTAACTACAACTTTGAAAAGCCCTTCC TCTGGCTTGCTAGGAAGCTCATTGGAGACCCTAACTTGGAATTTGTTGCCATGCCTGCTC TCGCCCCACCAGAAGTTGTCATGGACCCAGCTTTGGCAGCACAGTATGAGCACGACTTAG AGGTTGCTCAGACAACTGCTCTCCCGGATGAGGATGATGACCTGTGAGAATGAAGCTGGA GCCCAGCGTCAGAAGTCTAGTTTTATAGGCAGCTGTCCTGTGATGTCAGCGGTGCAGCGT GTGTGCCACCTCATTATTATCTAGCTAAGCGGAACATGTGCTTCATCTGTGGGATGCTGA AGGAGATGAGTGGGCTTCGGAGTGAATGTGGCAGTTTAAAAAATAACTTCATTGTTTGGA CCTGCATATTTAGCTGTTTTGGAACGCAGTTGATTCCTTGAGTTTCATATATAAGACTGC TGCAGTCACATCACAATATTCAGTGGTGAAATCTTGTTTGTTACTGTCATTCCCATTCCT TTTCGTTTAGAATCAGAATAAAGTTGTATTTCAAATATCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC016654 unedited GTCGGAATTTGTATACACTTACTATAGGCGGCCGCGNATTCANATCTGGTACCGGTCCGG AATTCCCGGGATATCGTCGACCCACGCGTCCGCGGGCGGCTGAGTAGGTGGGTGGTGCGG GCCCGGCCGGGCCGGGGCAGGAGACGGGCGTGGGGTCGGCGCTAGCCCCCGAGAACCCCC GTTTCATCCTCCGCTCTCATCCCCGTCCCGGTCCCAGTCCCGTTCCCATCCCTACACCTC CGTCCGCCGTTCCCCGGGCCCCGCCGCCCCGGATGCCGGCCCCGCCCGCCGCCTTCCCGC TCCCAGGCCTGGCCGCCATGGCGCCGCGGGCGGGAGGCCTTTGTGGGGCGGGCACGTGGG GCGCTGGGGGCGCGGGAGCGGGGCCGCCATGGGCTGCGGGGCCGCGCGAGCGCTCGCCTC CGTCCTCTGCCTCCGCAGGAACGCCGCGATGGCTGCGCAGGGAGAGCCCCAGGTCCAGTT CAAACTTGTATTGGTTGGTGATGGTGGTACTGGAAAAACGACCTTCGTGAAACGTCATTT GACTGGTGAATTTGAGAAGAAGTATGTAGCCACCTTGGGTGTTGAGGTTCATCCCCTAGT GTTCCACACCAACAGAGGACCTATTAAGTTCATGTATGGGACACAGCCGGCCAGGGAGAA TTCGGTGGACTGAGAGATGGCTATTATATCCAAGCCCAGTGTGCCCTCTTAATGTTTGAT GTAACATCGAAAGTAACTACCAGAATGTGCCTAACTGGCATAGAGATCTGGGACCAGTGT GGGAAAACATTCCCATTGGGTTGGGGGGCACCAAGGGGTTTTTAGGACGGGAAGGGAAGG CGAAATCCATTGGCTTCCCCCCGAAGAGAAATTTCATACTCCACATTTCTGCCCAAGTAA CCTCCCTTTGAAAAGCCTTTCCCCTGGCT
Restriction Sites	Please inquire
ACCN	BC016654
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC016654.1, AAH16654.1
RefSeq Size	1409 bp
Locus ID	5901
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008]

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