DLEU2 (NM_006021) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	1810047A16Rik; 4932411A06Rik; 7730401J12Rik; AI197277; AI788824; Alt1; deleted in lymphocytic leukemia, 2; DLB2, LEU2, BCMSUN, MGC88287; Leu2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_006021 edited GGGGAGCGCTTTCGGCGCCATTTTCGAGTGATGCCTGATCTCATCAATCTAGCGGGAGAG ACAGGATAACCTGTCCGAGAGTATAGCGCCACTATGACTCCGCCGGAAAAATTACTTTAA AAATCGCCAAAAATTACTTGGAGCAAAGGGCAGTCGGCGGAGCTTCGCCAAGGCTGGCGC AGTCGGTTTTGACCTGTAGCAGAGAACCAATTCTGGAGAACAGCCTCACTTCTTTGATTG AATACTTACATAATGCATTGGAACATGACATGAGATTAAGGTTTAATAATGATAGAATGA AGACCACAATAAAAGAGACCTCTACTTAGCTCAGCAATTCTTACCTTTCTTACCTATTTG ATGAAGATGTCTTTTGAAAGGTGTACTGCAAGGAACAAAATGTTTGTAAATTCTCCTTTT ACCAAGGTGGATAATTACTGTACCTTCCTCATGGAAAAAGTTTTATTTAAAGTGTTATTT CTCATTGAATACTATCAAAAAGGAAAAAAAAATGACCTAAACTTTTGAGATAGATTTGGC TCTAGTAAGTATTTAGTTATATCACTTGCATATCTGGGAGAAGAAATAAGAGACTATCAT CAGTACATTCCCATCTACTAAAAAAATTTATTTTACACATGTCAAGGGATTACTTATAAC TTCCATTTTATTACTAATAGCTTGAACCCTTTTAATGAAGACCTAACTCCTCCACCAGAA ATTTAAGTTTATGTTCTTACTTTGTTTACTTATAAAATACATCTCAGGTATTTCGGATGT CTTTTTTTTTTCTAAGCCTATATGAAATGAAAAATATATTGGCAAAATAAATGTTTAAAC CTTTTACGTTAAAATTACTTTGAAAGATGAAAAGTTAGTGCTGTTTTTGTCTACGTTATA CTGAAATTAAATGTTTATAATTTATATTTTGGGTTTATGTATAAATCATGGAATTTATGC AAAAATATGAGTAGTACAGATTCTCCTCTAATTCTGTAGGACTTTGAATAATGTGATATT TTTCTTATAATTGGACCCTTGTGTTTTGAAGAAATGCCAACTGCTTGAAGAATCTCCTTG TTATTTGTATTATTTGCTATAGGGTTAGATGTTGAGAAATTCTGCTGACAAAAAATTTTA AGCCAGTTTTACACTAAATGTTCCTCAGTCTGATTAATTTGTTATTGGATGTATTCTGTA TCTTTCTTTTGTAATTTGTAACTTTTATCCACTTAGCACGAATGATTCTATTAAAGAAAA TCATTAGGAAGTGGTAGAAACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_006021 unedited GGTCAGGATTTGTATACGACTCACTATAGGCGGCGCGCGAATTCGCCATTACGGCCGGGG AGCGCTTTCGGCGCCATTTTCGAGTGATGCCTGATCTCATCAATCTAGCGGGAGAGACAG GATAACCTGTCCGAGAGTATAGCGCCACTATGACTCCGCCGGAAAAATTACTTTAAAAAT CGCCAAAAATTACTTGGAGCAAAGGGCAGTCGGCGGAGCTTCGCCAAGGCTGGCGCAGTC GGTTTTGACCTGTAGCAGAGAACCAATTCTGGAGAACAGCCTCACTTCTTTGATTGAATA CTTACATAATGCATTGGAACATGACATGAGATTAAGGTGTAATAATGATAGAATGAAGAC CACAATAAAAGAGACCTCTACTTAGCTCAGCAATTCTTACCTTTCTTACCTATTTGATGA AGATGTCTTTTGAAAGGTGTACTGCAAGGAACAAAATGTTTGTAAATTCTCCTTTTACCA AGGTGGATAATTACTGTACCTTCCTCATGGAAAAAGTTTTATTTAAAGTGTTATTTCTCA TTGAATACTATCAAAAAGGAAAAAAAAATGACCTAAACTTTTGAGATAGATTTGGCTCTA GTAAGTATTTAGTTATATCACTTGCATATCTGGGAGAAGAAATAAGAGACTATCATCAGT ACATTCCCATCTACTAAAAAAATTTATTTTACACATGTGAAGGGATTACTTATAACTTCC ATTTTATTACTAATAGCGTGAACCCTTTTAATGAAGACCCTACTCCTNCACCAGAAATTT AAGTTTATGTGTCTACTTTGTGTACTTATAAAATACATCTCAGGTATTTCGGATGTGCTT TTTTTTTCTAGGCTATATGAAATGAAAATATATTGGGCAAATAATGTTTAAACCTTTACG GTAAATTTACTTNAAAGATGAAAAGTAATGCTGTTTTTGTCTACTTT
Restriction Sites	Please inquire
ACCN	NM_006021
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_006021.1, NP_006012.1
RefSeq Size	1750 bp
RefSeq ORF	255 bp
Locus ID	8847
Gene Summary	This locus represents a microRNA host gene and also produces long alternatively spliced non-coding RNAs. This genome region was observed to be deleted or epigenetically suppressed in leukemia, and was implicated as a negative regulator of cell proliferation. However, an alternative transcript produced at this locus was also found to promote progression through the cell cycle via angiotensin I converting enzyme 2 and cyclin D1. [provided by RefSeq, Dec 2017]

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