VPS29 (BC032462) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PEP11; vacuolar protein sorting 29; vacuolar protein sorting 29 homolog (S. cerevisiae)
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC032462 edited GTGGGCGGGTCGCCGAGGAGCCTGAGGAAGAGGGCGGCGACGGTGGTGGTGACTGAGCGG AGCCCGGTGACAGGATGGTGAGGGCTGCAGTTGCGGCGACCAAGGCCTGGCTCTCGGGTG GGGTGCTGCGTTGTTGGGAGCCGTGGGGAGGCCGAGGGCGAGGAATTGGGAAATCCCGGA GGCCTCGGCGTCAGGGCCCAGGCGCTGGCCTGGGAAAGGCCCAGAAGAGGTCTAGGGAAG GAAGGGAGCGGCCACCTCTGGGTGCGGCTGGCCTAGGTCTCGTATCCCGGCCCCAAGGGG CCAGAGGCCATCCAAGGGGTAAAAAGGCCCCTCTAGCAGCCCGACCTGTCACGAGACACG CACGCCCGAGCCAGGGAAGGCCTTTGGTCCTATACCTCGAATGTTCCGGGGATAGGGGCA CGGTCCTCCGTTTCCAGACGGTTCCTACTCGTTTCCCGCAAAAAGCACCTCCCGCTGGTT GGGGTGTAGTGGTGGAAGCTGGCCTCTCGGATGCAGAACAGGAAGTAGCGAGCAGAATTT CGTACCCGTTTAACTTTGCCATCTAGGGAAAGGGAATAAACCACCCTCCTCGCTCCTGAA ATAACATCTAGCTGCCATTGAGATGAGCAGGTGTGCTCTCAGAGGGCGGGATTTGGCGCT TGCAATTGCTGGAACTGTTTCTTTCCACGGGTTGCTACGCCTCTTTAGGGTAGGATTCCT TAATGATGTGTGAACTCATTGAAACTGTTGGGAAATTTTTGTGAATATACACATTTTTCT GGGGAGAGTGCCGGTGTTGGAGACTTACCTAGTCTCCCCCAGCCACCTTCAAGCAATCAC CCCTTCACCCCCAGTAGTTTGGTGCCCCTCAGACCTTCCTACATAAAAACTGGGGGCCTC TACTAGAGAGGACTCAGAGCTGTAATCATATTTTCTTTCTTTCTTTTTGAGATGGAGTCT CGCTCTGTCGCCCAGGCTGGAGTGCAATGCCGCGATCTCGGCTCACTGCAATGCCGCCCG GGTTCAAGCCTCAGCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCGCCCGCCA CCAAGCCCGTCTAAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGTCAGG CTGGTCTCTACCTCCCGACCTCAGATGATCCACCCGCCTCGGCCTCCCAAAGTGCTGGGG TTACAGGCGTGAGCCACCGCGCCCGGCCTGTAATCATATTTTCAAAGGTGCCTTTGACTC TTAAAAAGTCAATTGTTTGGGGCAACCCACTCGGGTCCCCTTCCACCCTGTGTAAGCTTT GTTCTTTCGCTCTTCACAATAAATCTTGCTGCTGCTCAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032462 unedited NGGTTCAACATTTGTAATACGACTCACTATAGGGNNCGGCCGCGNAATTCCCGGGNTGTG GGCGGGTCGCCGAGGAGCCTGAGGAAGAGGGCGGCGACGGTGGTGGTGACTGAGCGGAGC CCGGTGACAGGATGGTGAGGGCTGCAGTTGCGGCGACCAAGGCCTGGCTCTCGGGTGGGG TGCTGCGTTGTTGGGAGCCGTGGGGAGGCCGAGGGCGAGGAATTGGGAAATCCCGGAGGC CTCGGCGTCAGGGCCCAGGCGCTGGCCTGGGAAAGGCCCAGAAGAGGTCTAGGGAAGGAA GGGAGCGGCCACCTCTGGGTGCGGCTGGCCTAGGTCTCGTATCCCGGCCCCAAGGGGCCA GAGGCCATCCAAGGGGTAAAAAGGCCCCTCTAGCAGCCCGACCTGTCACGAGACACGCAC GCCCGAGCCAGGGAAGGCCTTTGGTCCTATACCTCGAATGTTCCGGGGATAGGGGCACGG TCCTCCGTTTCCAGACGGTTCCTACTCGTTTCCCGCAAAAAGCACCTCCCGCTGGTTGGG GTGTAGTGGTGGAAGCTGGCCTCTCGGATGCAGAACAGGAAGTAGCGAGCAGAATTTCGT ACCCGTTTAACTTTGCCATCTAGGGAAAGGGAATAAACCACCCTCCTCGCTCCTGAAATA ACATCTAGCTGCCATTGAGATGAGCAGGTGTGCTCTCAGAGGGCGGGATTTGGCGCTTGC AATTGCTGGAACTGTTTCTTTCCACGGGTTGCTACGCCTCTTTAGGGTANGATTCCTTAA TGATGTGTGAACTCATTGAAANCTGTTGGAAAATTTTGTGAATATACACATTTTTCTGGN GAGAGTGCCCGTGTTTGGAGACTTTACTAGTCTCCCCCAGCCACCTTCAAGACATCACCC CTTCACCCCCAGTATTTGGTGCCCCTCAGACCTTCTAC
Restriction Sites	Please inquire
ACCN	BC032462
Insert Size	1493 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC032462.1, AAH32462.1
RefSeq Size	1493 bp
Locus ID	51699
Gene Summary	This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene. [provided by RefSeq, Aug 2013]

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