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Supervillin (SVIL) (NM_003174) Human Untagged Clone
CAT＃: SC118113
SVIL (untagged)-Human supervillin (SVIL), transcript variant 1
CNY 12,024.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MFM10 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003174, the custom clone sequence may differ by one or more nucleotides
ATGAAAAGAAAAGAAAGAATTGCCAGGCGCCTGGAAGGGATTGAAAATGACACTCAGCCCATCCTCTTGC AGAGCTGCACAGGATTGGTGACTCACCGCCTGCTGGAGGAAGACACCCCTCGATACATGAGAGCCAGCGA CCCTGCCAGCCCCCACATCGGCCGATCAAATGAAGAGGAGGAAACTTCTGATTCTTCTCTAGAAAAGCAA ACTCGATCCAAATACTGCACAGAAACCTCCGGTGTCCACGGTGACTCACCCTATGGTTCGGGTACCATGG ACACCCACAGTCTGGAGTCCAAAGCCGAAAGAATTGCAAGGTACAAAGCAGAAAGAAGGCGACAGCTGGC AGAGAAGTATGGGCTGACTCTGGATCCCGAGGCCGACTCCGAGTATTTATCCCGCTATACCAAGTCCAGG AAGGAGCCTGATGCTGTCGAGAAGCGGGGAGGAAAAAGTGACAAACAGGAAGAGTCAAGCAGAGATGCTA GTTCTCTGTACCCCGGGACCGAGACGATGGGGCTCAGGACCTGTGCCGGTGAATCCAAGGACTATGCCCT CCATGTGGGTGACGGCTCTTCCGACCCGGAGGTGCTGCTGAACATAGAAAACCAAAGACGAGGTCAAGAG CTGAGTGCCACCCGGCAGGCCCATGACCTGTCCCCAGCAGCCGAGAGTTCCTCGACCTTCTCTTTCTCTG GGCGAGACTCCTCCTTCACTGAAGTGCCACGGTCCCCCAAGCACGCCCACAGCTCCTCCCTGCAGCAGGC AGCCTCCCGGAGCCCCTCCTTTGGTGACCCACAGCTATCCCCTGAGGCCCGACCCAGGTGCACTTCACAT TCAGAAACGCCAACTGTCGATGATGAAGAAAAGGTGGATGAACGAGCCAAGCTGAGCGTCGCCGCCAAGA GGTTGCTTTTCAGGGAGATGGAAAAATCTTTTGATGAACAAAATGTTCCAAAGCGACGCTCAAGAAACAC AGCTGTGGAGCAGAGGCTACGCCGTCTGCAGGACAGGTCCCTCACCCAGCCCATCACCACTGAAGAGGTG GTCATCGCAGCCACATTGCAGGCCTCTGCTCACCAAAAGGCCTTAGCCAAGGACCAGACAAATGAGGGCA AAGAGCTTGCTGAGCAAGGAGAACCTGATTCCTCCACTCTAAGCTTGGCCGAAAAGTTGGCCTTGTTTAA CAAATTGTCCCAGCCAGTCTCAAAAGCGATTTCTACCCGGAACAGAATAGACACGAGACAGAGGAGAATG AACGCTCGCTATCAAACTCAGCCAGTCACACTGGGAGAGGTGGAGCAGGTGCAGAGTGGAAAGCTCATTC CTTTCTCACCTGCCGTGAACACATCAGTGTCTACCGTAGCATCCACGGTTGCTCCAATGTATGCCGGAGA TCTTCGCACAAAGCCACCTCTTGACCACAATGCAAGTGCCACTGACTATAAGTTTTCTTCTTCAATAGAA AATTCGGACTCTCCAGTTAGAAGCATTCTGAAATCGCAAGCTTGGCAGCCTTTGGTAGAGGGTAGCGAGA ACAAGGGAATGTTGAGAGAATATGGAGAGACAGAAAGCAAGAGAGCTTTGACAGGTCGAGACAGTGGGAT GGAGAAGTATGGGTCCTTTGAGGAAGCAGAAGCATCCTACCCCATCCTGAACCGAGCCAGGGAAGGAGAC AGCCATAAGGAATCTAAATATGCTGTTCCCAGAAGAGGAAGCCTGGAACGGGCGAACCCTCCCATCACCC ACCTCGGGGATGAACCGAAGGAATTTTCCATGGCTAAAATGAATGCACAAGGAAACTTGGACTTGAGGGA CAGGCTGCCCTTTGAAGAGAAGGTGGAGGTGGAGAATGTTATGAAAAGGAAGTTTTCACTAAGAGCGGCA GAGTTCGGGGAGCCCACTTCCGAGCAGACGGGGACAGCTGCTGGGAAAACTATTGCTCAAACCACAGCCC CCGTGTCCTGGAAGCCCCAGGATTCTTCGGAACAGCCACAGGAGAAGCTCTGCAAGAATCCATGTGCGAT GTTTGCTGCTGGAGAGATCAAAACGCCGACAGGGGAGGGCCTTCTTGACTCACCCAGCAAAACCATGTCT ATTAAAGAAAGATTGGCACTGTTGAAGAAAAGCGGGGAGGAAGATTGGAGAAACAGACTCAGCAGGAGGC AGGAGGGCGGCAAGGCGCCGGCCAGCAGCCTGCACACCCAGGAAGCAGGGCGGTCCCTCATCAAGAAGCG GGTCACAGAAAGTCGAGAGAGCCAAATGACGATTGAGGAGAGGAAGCAGCTCATCACTGTGAGAGAGGAG GCCTGGAAGACGAGAGGCAGAGGAGCGGCCAACGACTCGACCCAGTTCACTGTGGCTGGCAGGATGGTGA AGAAAGGTTTGGCGTCACCTACTGCCATAACCCCAGTAGCCTCACCCATTTGCGGTAAAACAAGAGGCAC CACACCCGTTTCCAAACCCCTGGAAGATATCGAAGCCAGACCAGATATGCAGTTAGAATCGGACCTGAAG TTGGACAGGCTGGAAACCTTTCTAAGAAGGCTGAATAACAAAGTTGGCGGGATGCACGAAACGGTGCTCA CTGTCACCGGCAAATCTGTGAAGGAGGTGATGAAGCCAGATGATGATGAAACCTTTGCCAAATTTTACCG CAGCGTGGATTATAATATGCCAAGAAGTCCTGTGGAGATGGATGAGGACTTCGATGTCATTTTCGATCCT TATGCACCCAAATTGACGTCTTCCGTGGCCGAGCACAAGCGGGCAGTTAGGCCCAAGCGCCGGGTTCAGG CCTCCAAAAACCCCCTGAAAATGCTGGCGGCAAGAGAAGATCTCCTTCAGGAATACACTGAGCAGAGATT AAACGTTGCCTTCATGGAGTCAAAGCGGATGAAAGTAGAAAAGATGTCTTCCAACTCCAACTTCTCAGAA GTCACCCTGGCGGGTTTAGCCAGTAAAGAAAACTTCAGCAACGTCAGCCTGCGGAGCGTCAACCTGACGG AACAGAACTCTAACAACAGCGCCGTGCCCTACAAGAGGCTGATGCTGTTGCAGATTAAAGGAAGAAGACA TGTGCAGACCAGGCTGGTGGAACCTCGAGCTTCGGCGCTCAACAGTGGGGACTGCTTCCTCCTGCTCTCT CCCCACTGCTGCTTCCTGTGGGTAGGAGAGTTTGCAAACGTCATAGAAAAGGCGAAGGCCTCAGAACTTG CAACTTTAATTCAGACAAAGAGGGAACTTGGTTGTAGAGCTACTTATATCCAAACCATTGAAGAAGGAAT TAATACACACACTCATGCAGCCAAAGACTTCTGGAAGCTTCTGGGTGGCCAAACCAGTTACCAATCTGCT GGAGACCCAAAAGAAGATGAACTCTATGAAGCAGCCATAATAGAAACTAACTGCATTTACCGTCTCATGG ATGACAAACTTGTTCCTGATGACGACTACTGGGGGAAAATTCCGAAGTGCTCCCTTCTGCAACCCAAAGA GGTACTGGTGTTTGATTTTGGTAGTGAAGTTTACGTATGGCATGGGAAAGAAGTCACATTAGCACAACGA AAAATAGCATTTCAGCTGGCAAAGCACTTATGGAATGGAACCTTTGACTATGAGAACTGTGACATCAATC CCCTGGATCCTGGAGAATGCAATCCGCTTATCCCCAGAAAAGGACAGGGGCGGCCCGACTGGGCGATATT TGGGAGACTTACTGAACACAATGAGACGATTTTGTTCAAAGAGAAGTTTCTGGATTGGACGGAACTGAAG AGATCGAATGAGAAGAACCCCGGGGAACTTGCCCAGCACAAGGAAGACCCCAGGACTGATGTCAAGGCAT ACGATGTGACACGGATGGTGTCCATGCCCCAGACGACAGCAGGCACCATCCTGGACGGAGTGAACGTCGG CCGTGGCTATGGCCTGGTGGAAGGACACGACAGGAGGCAGTTTGAGATCACCAGCGTTTCCGTGGATGTC TGGCACATCCTGGAATTCGACTATAGCAGGCTCCCCAAACAAAGCATCGGGCAGTTCCATGAGGGGGATG CCTATGTGGTCAAGTGGAAGTTCATGGTGAGCACGGCAGTGGGAAGTCGCCAGAAGGGAGAGCACTCGGT GAGGGCAGCCGGCAAAGAGAAGTGCGTCTACTTCTTCTGGCAAGGCCGGCACTCCACCGTGAGTGAGAAG GGCACGTCGGCGCTGATGACGGTGGAGCTGGACGAGGAAAGGGGGGCCCAGGTCCAGGTTCTCCAGGGAA AGGAGCCCCCCTGTTTCCTGCAGTGTTTCCAGGGGGGGATGGTGGTGCACTCGGGGAGGCGGGAAGAGGA AGAAGAAAATGTGCAAAGTGAGTGGCGGCTGTACTGCGTGCGTGGAGAGGTGCCCGTGGAAGGGAATTTG CTGGAAGTGGCCTGTCACTGTAGCAGCCTGAGGTCCAGAACTTCCATGGTGGTGCTTAACGTCAACAAGG CCCTCATCTACCTGTGGCACGGATGCAAAGCCCAGGCCCACACGAAGGAGGTCGGAAGGACCGCTGCGAA CAAGATCAAGGAACAATGTCCCCTGGAAGCAGGACTGCATAGTAGCAGCAAAGTCACAATACACGAGTGT GATGAAGGCTCCGAGCCACTCGGATTCTGGGATGCCTTAGGAAGGAGAGACAGGAAAGCCTACGATTGCA TGCTTCAAGATCCTGGAAGTTTTAACTTCGCGCCCCGCCTGTTCATCCTCAGCAGCTCCTCTGGGGATTT TGCAGCCACAGAGTTTGTGTACCCTGCCCGAGCCCCCTCTGTGGTCAGTTCCATGCCCTTCCTGCAGGAA GATCTGTACAGCGCGCCCCAGCCAGCACTTTTCCTTGTTGACAATCACCACGAGGTGTACCTCTGGCAAG GCTGGTGGCCCATCGAGAACAAGATCACTGGTTCCGCCCGCATCCGCTGGGCCTCCGACCGGAAGAGTGC GATGGAGACTGTGCTCCAGTACTGCAAAGGAAAAAATCTCAAGAAACCAGCCCCCAAGTCTTACCTTATC CACGCTGGTCTGGAGCCCCTGACATTCACCAATATGTTTCCCAGCTGGGAGCACAGAGAGGACATCGCTG AGATCACAGAGATGGACACGGAAGTTTCCAATCAGATCACCCTCGTGGAAGACGTCTTAGCCAAGCTCTG TAAAACCATTTACCCGCTGGCCGACCTCCTGGCCAGGCCACTCCCGGAGGGGGTCGATCCTCTGAAGCTT GAGATCTATCTCACCGACGAAGACTTCGAGTTTGCACTAGACATGACGAGGGATGAATACAACGCCCTGC CCGCCTGGAAGCAGGTGAACCTGAAGAAAGCAAAAGGCCTGTTCTGA >OriGene 5' read for NM_003174 unedited
GTTCACTATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCATCCTGT GCGGGGACCCGCTCCCCGCGCGGTGGGAACGAGGCGGGGCGTGGCCGCGGCGCTGCTGAG AAGTCGGGGCCGCCGAGCCGCTGTCGGCAGGAAGCGGCGATCCTGCCACCGGGAGGTGTG GAAGAGCCGGATTCTGGCTACATTGGAGATTGGTTGCTTTCTAAAACTGAAGGAGAAGCC CATGAAGAGATGGTGGATTCTCACTGAGTTTTGACTAGCGGAAGAAAAGAGAGAGTTCAA GTGGATGGCCTTGAGGACTTGAAAAGCTGAGATATGATGATTTTGAAGTCATTTCACATC GAAGCCATGATTTAAATATCGGCGTTAAGATTTCAACAAGAAAAACTTAAGCTTCCTTGG ATTCCCACGTCAAAGGAAAGTTTCAAGCTTTCACAAGGAGTTCTCACTCGAAGATAAAGA ACAGCTCGCTAACCACGAAAGAGGAATCGATGCTCAGCTTTTAGTTGCACTTCCTAAAGT TGCAGAATTAAGACAAATCTTTGAACCAAAGAAGAAAGAATTCTTAGAAATGAAAAGAAA AGAAAGAATTGCCAGGCGCCTGGAAGGGATTGAAAATGACACTCAGCCCATCCTCTTGCA GAGCTGCACAGGATTGGTGACTCACCGCCTGCTGGAGGAAGACACCCCTCGATACATGAA AGCCAGCGACCCTGCCAGCCCCCACATCGGCCGATCCAATGAAGAGGAGGAAACTTCTGA TTCTTCTCTAGAAAAGCAGACTCGATTCAAATACTGCACAGAAACCTTCCGTGTCCACGG TGACTCACCTTATGTTTCCGTTCCATGGACCCCACAGTCTTGAGTTCAAACCCGAAGCAT TGCAAGGTACAAAGCAAAAC >OriGene 3' read for NM_003174 unedited
TACTATGGACGCGGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTATTAGAAAATT GAGATGTTTTTATTTTGACTATTTGAGAGTAAAATTGTAGATAATGTTCCAAACCATCAT CAAAAATGCAGAGCAAACTTTTCTGTATATAAACTGTACATAAAAACAAGGCACTATACA TTTTTGGGGAGATATTAAGGTAGAAAGGTTGATTCGCATAGATTCTCCAGAGTCCATGCC ATTAAGTACAAATTCTTTGTTCATTTTAGCACCGGAAGCAGTACTCAGAACAAGTCATAC CTAATGCTGCAGGAAACATTTCCAAACGTGTATGAAGATACATATTGGTGGCAGAGCTAA TTCAACAGAAGCAACTCCATCTACCTTTTCATGTTATTTTGACACCAAAACAAATCTTGA AAGTGAATGAATACATATTGCTTTGTTAAATACATATTTGACTTATATGGTGTTTATATA AATATATATATATTTTAGAATCCACAAACTATCAAAATAACACTTTATAAAGAGAACTGC TTCAAAAAAAAAAAAAAAAGGCATTGCCTAGCTGGAACAAGAAGGCAGTGCTATCTATAG CAGCTGCGGCTTAAGTGCACATAACAGATACTTTTATTAATTCTGATAACCTCCTGAATG GTGGAAAGAAGTTTCCAAACAGTTCCCTTGAACATTTACAAAATACACAACTCCGGGACA AGCAGTATCTTTAACAATGTCAGGTTCTGAAAACTCTGATTGAAAAATACTTTGTGAAAA ACACCAGTCCAAAAAATATATCCATTTTCCTGGTGCATAGGTGTTGTTGGACGTGACCGT GAAGCTCCTCTGGCGTCTCCCACTCAGACAGGCCTTTTGCTTTCTCAGGTCACCTGCTTC CAGCGGGGCAGGCGTGTATTCATCCTCGTCATGCTATGCAAATCGAAGCCTCGCCGTGAG AAGATCCAGCTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003174 |
| Insert Size | 6250 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003174.2, NP_003165.1 |
| RefSeq Size | 6719 bp |
| RefSeq ORF | 5367 bp |
| Locus ID | 6840 |
| UniProt ID | O95425 |
| Domains | VHP, GEL, Gelsolin |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a bipartite protein with distinct amino- and carboxy-terminal domains. The amino-terminus contains nuclear localization signals and the carboxy-terminus contains numerous consecutive sequences with extensive similarity to proteins in the gelsolin family of actin-binding proteins, which cap, nucleate, and/or sever actin filaments. The gene product is tightly associated with both actin filaments and plasma membranes, suggesting a role as a high-affinity link between the actin cytoskeleton and the membrane. The encoded protein appears to aid in both myosin II assembly during cell spreading and disassembly of focal adhesions. Several transcript variants encoding different isoforms of supervillin have been described. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (1) is alternatively spliced in the 5' portion of the gene, resulting in the shorter isoform (1) which has a different amino-terminus compared to isoform 2. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216323 | SVIL (Myc-DDK-tagged)-Human supervillin (SVIL), transcript variant 1 |
CNY 12,016.00 |
|
| RC216323L1 | Lenti ORF clone of Human supervillin (SVIL), transcript variant 1, Myc-DDK-tagged |
CNY 14,416.00 |
|
| RC216323L3 | Lenti ORF clone of Human supervillin (SVIL), transcript variant 1, Myc-DDK-tagged |
CNY 19,760.00 |
|
| RG216323 | SVIL (tGFP-tagged) - Human supervillin (SVIL), transcript variant 1 |
CNY 13,616.00 |
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