United States
Germany
Japan
United Kingdom
China
GCSH (NM_004483) Human Untagged Clone
CAT＃: SC117387
GCSH (untagged)-Human glycine cleavage system protein H (aminomethyl carrier) (GCSH), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 2,400.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GCE; NKH |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117387 sequence for NM_004483 edited (data generated by NextGen Sequencing)
ATGGCGCTGCGAGTGGTGCGGAGCGTGCGGGCCCTGCTCTGCACCCTGCGCGCGGTCCCG TTACCCGCCGCGCCCTGCCCGCCGAGGCCCTGGCAGCTGGGGGTGGGCGCCGTCCGTACG CTGCGCACTGGACCCGCTCTGCTCTCGGTGCGTAAATTCACAGAGAAACACGAATGGGTA ACAACAGAAAATGGCATTGGAACAGTGGGAATCAGCAATTTTGCACAGGAAGCGTTGGGA GATGTTGTTTATTGTAGTCTCCCTGAAGTTGGGACAAAATTGAACAAACAAGATGAGTTT GGTGCTTTGGAAAGTGTGAAAGCTGCTAGTGAACTCTATTCTCCTTTATCAGGAGAAGTA ACTGAAATTAATGAAGCTCTTGCAGAAAATCCAGGACTTGTAAACAAATCTTGTTATGAA GATGGTTGGCTGATCAAGATGACACTGAGTAACCCTTCAGAACTAGATGAACTTATGAGT GAAGAAGCATATGAGAAATACATAAAATCTATTGAGGAGTGA Clone variation with respect to NM_004483.4 62 c=>t >OriGene 5' read for NM_004483 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCAAGAGGCCGCACCCCTGCGAA CATGGCGCTGCGAGTGGTGCGGAGCGTGCGGGCCCTGCTCTGCACCCTGCGCGCGGTCCC GTTACCCGCCGCGCCCTGCCCGCCGAGGCCCTGGCAGCTGGGGGTGGGCGCCGTCCGTAC GCTGCGCACTGGACCCGCTCTGCTCTCGGTGCGTAAATTCACAGAGAAACACGAATGGGT AACAACAGAAAATGGCATTGGAACAGTGGGAATCAGCAATTTTGCACAGGAAGCGTTGGG AGATGTTGTTTATTGTAGTCTCCCTGAAGTTGGGACAAAATTGAACAAACAAGATGAGTT TGGTGCTTTGGAAAGTGTGAAAGCTGCTAGTGAACTCTATTCTCCTTTATCAGGAGAAGT AACTGAAATTAATGAAGCTCTTGCAGAAAATCCAGGACTTGTAAACAAATCTTGTTATGA AGATGGTTGGCTGATCAAGATGACACTGAGTAACCCTTCAGAACTAGATGAACTTATGAG TGAAGAAGCATATGAGAAATACATAAAATCTATTGAGGAGTGAAAATGGAACTCCTAAAT AAACTAGTATGAAATAACGCAAGCCAGCAGAGTTGTCTTAAATTAGTGGTGGATAGAAGA CTTAGAATAGAACTTTTAGTATTACCGATGGGGAAAAAAAAACTACTGTTAACACTGCTA ATGAAAGAAAATGCCCTTTAACTNTCTAATGATTATAGATAAATATAANTATGCGTCTTT NTCACAATATCCTATGATTNNTAGACTANGCTNCTAGTGGTCAGAAATCATNNGAATATN CATGGNTANAACTANGTATAAAAATTACATAATTCAAGATNACATTGNTATNCNTAAGCC TATNNTATATNGNCACTG >OriGene 3' read for NM_004483 unedited
CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAACGAAGAAGTATTT TATTATTTTGCTGCAAAGCTGTTGCTTCACTGTATAAAAATAGCACCAGCAAATGCAGTG TATCGCAAAATTAAGATAGTGGTGTTCCTCATCTGACACTGTACAAGCAACAAAACCTCT TCACTTCCAGTTATTTCCAATGGAAAGATCATTAAGTATTTCATCCCAAATCCAGGTATG GATACATGCAAGTTACAATATTATATAAGGCTTAAGAATAACAATGTTATCTTTGAATTA TGTAATTTTTATAACTAGTTTTTACCATGGATAATTTCATGAATTCTGAACACTAGAGCC TAGTCTAAAAATCATAGGATATTGTGAAAAAGACGCATATTATATTTATCTATAATCATT AGAAAGTTAAAGGGCATTTTCTTTCATTAGCAGTGTTAACAGTAGTTTTTTTTTCCCCAT CGGTAATACTAAAAGTTTCTATTCTAAGTCTTCTATCCACCACTAATTTAAGACAACTCT GCTGGCTTGCGTTATTTCATACTAGTTTATTTAGGAGTTCCATTTTCACTCCTCAATAGA TTTATGATTTCTCAATGCCTTTCACTCATAGTTCATCTAGTTCTGAAGGGTTACTCAGTG GCATTTTTGATCAGCCAACCATCTTCATACAAAATTTGTTTACAAGCCCTGGATTTTTTG CAGACTTCATAATTTCCGTACTTCCCCTGATAAAGAGAATAGAGTTACTTNCAGTTTTAC ACTTTCCAAAGCACCAAATATTTTGGTTGGTCAATTTGGCCCAACTTAGGGAAATACATA AAAACATTTCCAAGCGTTCTGGGAAAATGGGGATCCACTGTTCAAGCCATTTTGTTGGCC CCTCCCGTTTTCTTGAAATACCCCCAAAACAACGGTCCAGGCAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004483 |
| Insert Size | 1150 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004483.3, NP_004474.2 |
| RefSeq Size | 1161 bp |
| RefSeq ORF | 522 bp |
| Locus ID | 2653 |
| UniProt ID | P23434 |
| Domains | GCV_H |
| Gene Summary | Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for this gene. Also, several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.[provided by RefSeq, Jan 2010] Transcript Variant: This variant (1) represents the longer transcript and is the protein-coding variant. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217886 | GCSH (Myc-DDK-tagged)-Human glycine cleavage system protein H (aminomethyl carrier) (GCSH), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 2,400.00 |
|
| RC217886L3 | Lenti ORF clone of Human glycine cleavage system protein H (aminomethyl carrier) (GCSH), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217886L4 | Lenti ORF clone of Human glycine cleavage system protein H (aminomethyl carrier) (GCSH), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG217886 | GCSH (tGFP-tagged) - Human glycine cleavage system protein H (aminomethyl carrier) (GCSH), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,000.00 |
