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TRMT12 (NM_017956) Human Untagged Clone
CAT＃: SC113870
TRMT12 (untagged)-Human tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12)
CNY 3,656.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | TRM12; TYW2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113870 sequence for NM_017956 edited (data generated by NextGen Sequencing)
ATGAGAGAGAATGTGGTTGTTAGCAACATGGAGAGAGAAAGTGGGAAGCCCGTGGCTGTT GTCGCAGTTGTGACTGAGCCTTGGTTTACCCAGCGATACAGAGAATATCTCCAGAGGCAG AAACTCTTTGATACACAGCACCGTGTGGAAAAGATGCCGGATGGCTCGGTGGCGCTACCG GTGCTGGGAGAGACGCTTCCAGAGCAGCACCTGCAGGAGCTGAGGAATCGTGTTGCCCCA GGCAGTCCCTGTATGCTCACGCAGCTCCCGGATCCTGTTCCTTCGAAGAGGGCCCAGGGT TGTTCACCTGCCCAAAAATTGTGTCTTGAGGTGAGTCGCTGGGTGGAGGGTCGGGGAGTC AAGTGGTCAGCCGAGTTGGAGGCTGATTTGCCCCGATCATGGCAACGGCATGGTAATCTC TTGTTGCTGAGTGAAGACTGTTTCCAAGCCAAGCAGTGGAAAAATCTGGGACCGGAACTC TGGGAGACCGTTGCCTTGGCACTTGGCGTCCAGCGTTTGGCAAAACGAGGGCGGGTATCA CCGGATGGTACTCGAACTCCAGCAGTGACACTGCTGCTGGGTGACCATGGCTGGGTAGAG CATGTGGATAATGGTATCCGTTATAAGTTTGACGTGACCCAGTGTATGTTCTCCTTTGGA AACATCACTGAGAAGCTTCGAGTGGCATCGTTGTCCTGTGCTGGAGAAGTGCTGGTGGAT CTCTATGCAGGGATTGGTTATTTTACATTGCCTTTCCTAGTTCATGCTGGTGCTGCCTTC GTCCATGCTTGTGAGTGGAATCCCCATGCTGTAGTTGCTCTGAGAAATAACCTTGAGATC AATGGAGTAGCAGATCGGTGCCAAATACACTTTGGAGATAACAGAAAACTGAAGCTCTCA AATATTGCAGATAGGGTGATCCTGGGGCTGATTCCCAGCTCTGAAGAAGGCTGGCCCATT GCCTGCCAAGTGTTAAGGCAGGATGCTGGAGGCATTTTGCATATCCACCAAAATGTGGAA TCTTTCCCAGGGAAGAATCTTCAGGCTCTTGGAGTCAGCAAAGTAGAGAAAGAGCATTGG CTGTATCCTCAGCAAATTACCACCAACCAATGGAAAAATGGAGCTACCAGGGATTCTAGG GGAAAAATGCTGTCACCAGCCACCAAGCCAGAGTGGCAAAGGTGGGCAGAATCTGCAGAA ACTCGAATCGCCACTCTTCTTCAGCAGGTGCATGGGAAACCATGGAAGACACAAATTCTG CACATCCAACCAGTGAAATCCTATGCTCCCCATGTGGATCACATAGTCCTGGATCTGGAA TGCTGCCCCTGTCCTTCAGTTGGCTAG Clone variation with respect to NM_017956.3 >OriGene 5' read for NM_017956 unedited
GGGGTCAGATTTGTATACGACTTCTATAGGCGGCCGCGNAATTCGCACGAGGGCCGGGTG AGCTGCAGGCTACCTTATTTAAGACCGGGAATTTAGTCAGCCTGGTGAGCCGACTCTGAG GAGATGGAGTATCGCTGAGGTGATGAGAGAGAATGTGGTTGTTAGCAACATGGAGAGAGA AAGTGGGAAGCCCGTGGCTGTTGTCGCAGTTGTGACTGAGCCTTGGTTTACCCAGCGATA CAGAGAATATCTCCAGAGGCAGAAACTCTTTGATACACAGCACCGTGTGGAAAAGATGCC GGATGGCTCGGTGGCGCTACCGGTGCTGGGAGAGACGCTTCCAGAGCAGCACCTGCAGGA GCTGAGGAATCGTGTTGCCCCAGGCAGTCCCTGTATGCTCACGCAGCTCCCGGATCCTGT TCCTTCGAAGAGGGCCCAGGGTTGTTCACCTGCCCAAAAATTGTGTCTTGAGGTGAGTCG CTGGGTGGAGGGTCGGGGAGTCAAGTGGTCAGCCGAGTTGGAGGCTGATTTGCCCCGATC ATGGCAACGGCATGGTAATCTCTTGTTGCTGAGTGAAGACTGTTTCCAAGCCAAGCAGTG GAAAAATCTGGGACCGGAACTCTGGGAGACCGTTGCCTTGGCACTTGGCGTCCAGCGTTT GGCAAAACGAGGGCGGGTATCACCGGATGGTACTCGAACTCCAGCAGTGACACTGCTGCT GGGTGACCATGGCTGGGTAGAGCATGTGGATAATGGTATCCGTTATAAGTTTGACGTGAC CCAGTGTATGTTCTCCTTTGGAAACATCACTGAGAAGCTTCGAGTGGCATCGTTGTCCTG TGCTNGGAGAGTGCTGGGTGGATCTCTATGCAGGNATTGGTTATTTTACATTGCCTTTNC TAGNTCATGCTGTGCTGNCTTCGTCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017956 |
| Insert Size | 2200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017956.1, NP_060426.1 |
| RefSeq Size | 2061 bp |
| RefSeq ORF | 1347 bp |
| Locus ID | 55039 |
| UniProt ID | Q53H54 |
| Domains | Met_10 |
| Gene Summary | Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203982 | TRMT12 (Myc-DDK-tagged)-Human tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12) |
CNY 3,656.00 |
|
| RC203982L3 | Lenti ORF clone of Human tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203982L4 | Lenti ORF clone of Human tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12), mGFP tagged |
CNY 5,890.00 |
|
| RG203982 | TRMT12 (tGFP-tagged) - Human tRNA methyltransferase 12 homolog (S. cerevisiae) (TRMT12) |
CNY 5,256.00 |
