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TMEM111 (EMC3) (NM_018447) Human Untagged Clone
CAT＃: SC113519
EMC3 (untagged)-Human transmembrane protein 111 (TMEM111)
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | POB; TMEM111 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113519 sequence for NM_018447 edited (data generated by NextGen Sequencing)
ATGGCAGGGCCAGAACTGTTGCTCGACTCCAACATCCGCCTCTGGGTGGTCCTACCCATC GTTATCATCACTTTCTTCGTAGGCATGATCCGCCACTACGTGTCCATCCTGCTGCAGAGC GACAAGAAGCTCACCCAGGAACAAGTATCTGACAGTCAAGTCCTAATTCGAAGCAGAGTC CTCAGGGAAAATGGAAAATACATTCCCAAACAGTCTTTCTTGACACGAAAATATTATTTC AACAACCCAGAGGATGGATTTTTCAAAAAAACTAAACGGAAGGTAGTGCCACCTTCTCCT ATGACTGATCCTACTATGTTGACAGACATGATGAAAGGGAATGTAACAAATGTCCTCCCT ATGATTCTTATTGGTGGATGGATCAACATGACATTCTCAGGCTTTGTCACAACCAAGGTC CCATTTCCACTGACCCTCCGTTTTAAGCCTATGTTACAGCAAGGAATCGAGCTACTCACA TTAGATGCATCCTGGGTGAGTTCTGCATCCTGGTACTTCCTCAATGTATTTGGGCTTCGG AGCATTTACTCTCTGATTCTGGGCCAAGATAATGCCGCTGACCAATCACGAATGATGCAG GAGCAGATGACGGGAGCAGCCATGGCCATGCCCGCAGACACAAACAAAGCTTTCAAGACA GAGTGGGAAGCTTTGGAGCTGACGGATCACCAGTGGGCACTAGATGATGTCGAAGAAGAG CTCATGGCCAAAGACCTCCACTTCGAAGGCATGTTCAAAAAGGAATTACAGACCTCTATT TTTTGA Clone variation with respect to NM_018447.2 >OriGene 5' read for NM_018447 unedited
CGGTTCACATTTGTAAACGACTCACTATAGGCGGCCGCGCAATTCGCACGAGGGCCGGNA GGCGAAGCCTGAAAAGAAGCAACTGTGTCCCCGGTAAAAGAGAAGCTCGCCCATTCCAGA CTGGGAACCAGCTTTCAGTGAAGATGGCAGGGCCAGAACTGTTGCTCGACTCCAACATCC GCCTCTGGGTGGTCCTACCCATCGTTATCATCACTTTCTTCGTAGGCATGATCCGCCACT ACGTGTCCATCCTGCTGCAGAGCGACAAGAAGCTCACCCAGGAACAAGTATCTGACAGTC AAGTCCTAATTCGAAGCAGAGTCCTCAGGGAAAATGGAAAATACATTCCCAAACAGTCTT TCTTGACACGAAAATATTATTTCAACAACCCAGAGGATGGATTTTTCAAAAAAACTAAAC GGAAGGTAGTGCCACCTTCTCCTATGACTGATCCTACTATGTTGACAGACATGATGAAAG GGAATGTAACAAATGTCCTCCCTATGATTCTTATTGGTGGATGGATCAACATGACATTCT CAGGCTTTGTCACAACCAAGGTCCCATTTCCACTGACCCTCCGTTTTAAGCCTATGTTAC AGCAAGGAATCGAGCTACTCACATTAGATGCATCCTGGGTGAGTTCTGCATCCTGGTACT TCCTCAATGTATTTGGGCTTCGGAGCATTTACTCTCTGATTCTGGGCCAAGAAATGCCGC TGACCAATCACGAATGATGCAGGAGCAGATGACGGGAGCAGCCATGGCCATGCCCGCAGA CACAAACAAAGCTTTTCAGACCGAGTGGGAAGCTTTGGAGCTGACGGACCACCAGTGGGC ACTAATAGATGTCCTAAGAGAGCTCATGGCCAAAAGACCTTCACTTCAAGGGCATGTCAC N >OriGene 3' read for NM_018447 unedited
AGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCT ATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAGATGGAGTTTTG CTCTGTCGCCCAGGCTGGAGCGCAGTGGCACGATGTCAGCTCACTGCATCCTCCGCCTCC TAGGTTTAAGCAGTTCTCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCCCACCA CCACGCCCGGCTAATTTTTTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATCTTGGCC AGGCTGATCTTGAACTCCTGATCTCGTGACCCACCCGCCTCAGCCTCCCAAAATCCTGGG ATTACAGGCGTGAGCCACTGCGCCTGGCCAAGATTTTATATATTATCAGTAGCCTGAGGT TTCCCCCTTTCTCTGACTTTCATTACTAGAGNCACCAGAAGGAACATTTACAACATTTTA AAAATAACAAGTTGCCCAGCATACTCCTATTTCCTCTAGTTTCAAACATAAAGGGGAACC CAGCCCAGACAAGAACAAGCCTTGCTGCATGCCTGCCAGCTCGTGCATCCTCCTTTTTAT TTCAAGAGGTGCCCGCTCCAAACAAAGTTACAAGGGTTAGTGCAACTCCAAGTTCCTGAC ACAGCTAATCCCTGCTCGGTCTTCAAAAATAGAGGTCTGTAATCCCTTTTGGACATGCCT TCGAGGGGNAGGCTTTGCCATGAGCTCTCTTCGACATATCTAGTGCCACTGTGATCCGCA ACTCCAAGCTCCCCTTTGCTGAAGCTTGTTTGGGTTGCGGCATGCCATGTTGCCCCGCAT TGCTCTGATAATTGGATTGGCAGGGATTACCTGCCAAATTAAAAGTATGCTCCACCCCAT CTGGGGATCCAGTGCCACCCCCAGAGCCTATGGAAGCCGTCCTGCGACTAGCTAACGGGC ATGGATGGACTGTTGGAAACGAATTTGTGCCTCCAAGATTAGGGACTGTCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018447 |
| Insert Size | 1500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018447.1, NP_060917.1 |
| RefSeq Size | 1109 bp |
| RefSeq ORF | 786 bp |
| Locus ID | 55831 |
| UniProt ID | Q9P0I2 |
| Protein Families | Transmembrane |
| Gene Summary | Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206832 | EMC3 (Myc-DDK-tagged)-Human transmembrane protein 111 (TMEM111) |
CNY 2,400.00 |
|
| RC206832L3 | Lenti ORF clone of Human transmembrane protein 111 (TMEM111), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206832L4 | Lenti ORF clone of Human transmembrane protein 111 (TMEM111), mGFP tagged |
CNY 5,890.00 |
|
| RG206832 | EMC3 (tGFP-tagged) - Human transmembrane protein 111 (TMEM111) |
CNY 4,000.00 |
|
| SC324507 | EMC3 (untagged)-Human transmembrane protein 111 (TMEM111) |
CNY 2,400.00 |
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