United States
Germany
Japan
United Kingdom
China
MRPS15 (NM_031280) Human Untagged Clone
CAT＃: SC107657
MRPS15 (untagged)-Human mitochondrial ribosomal protein S15 (MRPS15), nuclear gene encoding mitochondrial protein
CNY 2,400.00
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DC37; MPR-S15; RPMS15; S15mt |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC107657 sequence for NM_031280 edited (data generated by NextGen Sequencing)
ATGCTGAGGGTCGCGTGGAGGACGCTGAGTTTGATTCGGACCCGGGCAGTTACCCAGGTC CTAGTACCCGGGCTGCCGGGCGGTGGGAGCGCCAAGTTTCCTTTCAACCAGTGGGGCCTG CAGCCTCGAAGTCTCCTCCTCCAGGCCGCGCGCGGATATGTCGTCCGGAAACCAGCCCAG TCTAGGCTGGATGATGACCCACCTCCTTCTACGCTGCTCAAAGACTACCAGAATGTCCCT GGAATTGAGAAGGTTGATGATGTCGTGAAAAGACTCTTGTCTTTGGAAATGGCCAACAAG AAGGAGATGCTAAAAATCAAGCAAGAACAGTTTATGAAGAAGATTGTTGCAAACCCAGAG GACACCAGATCCCTGGAGGCTCGAATTATTGCCTTGTCTGTCAAGATCCGCAGTTATGAA GAACACTTGGAGAAACATCGAAAGGACAAAGCCCACAAACGCTATCTGCTAATGAGCATT GACCAGAGGAAAAAGATGCTCAAAAACCTCCGTAACACCAACTATGATGTCTTTGAGAAG ATATGCTGGGGGCTGGGAATTGAGTACACCTTCCCCCCTCTGTATTACCGAAGAGCCCAC CGCCGATTCGTGACCAAGAAGGCTCTGTGCATTCGGGTTTTCCAGGAGACTCAAAAGCTG AAGAAGCGAAGAAGAGCCTTAAAGGCTGCAGCAGCAGCCCAAAAACAAGCAAAGCGGAGG AACCCAGACAGCCCTGCCAAAGCCATACCAAAGACACTCAAAGACAGCCAATAA Clone variation with respect to NM_031280.3 207 c=>t >OriGene 5' read for NM_031280 unedited
AGAATTTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGCGGCGCCCA TGTAACCCGGTCCGTGCCGCAAAGCGAACGGCGGCCGCGGCGCGGGCCCCGCGGGGGTTA GAGGTCACCATGCTGAGGGTCGCGTGGAGGACGCTGAGTTTGATTCGGACCCGGGCAGTT ACCCAGGTCCTAGTACCCGGGCTGCCGGGCGGTGGGAGCGCCAAGTTTCCTTTCAACCAG TGGGGCCTGCAGCCTCGAAGTCTCCTCCTCCAGGCCGCGCGCGGATATGTCGTCCGGAAA CCAGCCCAGTCTAGGCTGGATGATGACCCACCTCCTTCTACGCTGCTCAAAGACTACCAG AATGTCCCTGGAATTGAGAAGGTTGATGATGTCGTGAAAAGACTCTTGTCTTTGGAAATG GCCAACAAGAAGGAGATGCTAAAAATCAAGCAAGAACAGTTTATGAAGAAGATTGTTGCA AACCCAGAGGACACCAGATCCCTGGAGGCTCGAATTATTGCCTTGTCTGTCAAGATCCGC AGTTATGAAGAACACTTGGAGAAACATCGAAAGGACAAAGCCCACAAACGCTATCTGCTA ATGAGCATTGACCAGAGGAAAAAGATGCTCAAAAACCTCCGTAACACCAACTATGATGTC TTTGAGAAGATATGCTGGGGGCTGGGAATTGAGTACACCTTCCCCCCTCTGTATTACCGA AGAGCCCACCGCCGATTCGTGACCAAGAAGGCTCTGTGCATTCGGGTTTTCCAGGAGACT CANAAGCTGAAGAAGCGAAAAAGAGCCTTANAGCTGCAGCAGCAGCCCAAAAACAAAGCA AGCGGNAGAACCCNAGACAGCCTGCCAAAGCCATACCANAGACACTCAAGACAGNNCATA NATTTCTGTCATCATTAANAAAAAACCCTAGATTGCGGNNCGCGTCATAGCTTGTTTCTT GACAGATCCCGGNNTGGCATCNCTGTGACCNCTCCC >OriGene 3' read for NM_031280 unedited
NNNNNNNNNGGGTTAAACTATGGNACCGCGGCCGCATNCTAGNATTTTTTTTTTAAGATT GAACAGAATTTATTGGCTGTCTTTGAGTGTCTTTGGTATGGCTTTGGCAGGGCTGTCTGG GTTCCTCCGCTTTGCTTGTTTTTGGGCTGCTGCTGCAGCCTTTAAGGCTCTTCTTCGCTT CTTCAGCTTTTGAGTCTCCTGGAAAACCCGAATGCACAGAGCCTTCTTGGTCACGAATCG GCGGTGGGCTCTTCGGTAATACAGAGGGGGGAAGGTGTACTCAATTCCCAGCCCCCAGCA TATCTTCTCAAAGACATCATAGTTGGTGTTACGGAGGTTTTTGAGCATCTTTTTCCTCTG GTCAATGCTCATTAGCAGATAGCGTTTGTGGGCTTTGTCCTTTCGATGTTTCTCCAAGTG TTCTTCATAACTGCGGATCTTGACAGACAAGGCAATAATTCGAGCCTCCAGGGATCTGGT GTCCTCTGGGTTTGCAACAATCTTCTTCATAAACTGTTCTTGCTTGATTTTTAGCATCTC CTTCTTGTTGGCCATTTCCAAAGACAAGAGTCTTTTCACGACATCATCAACCTTCTCAAT TCCAGGGACATTCTGGTAGTCTTTGAGCAGCGTAGAAGGAGGTGGGTCATCATCCAGCCT AGACTGGGCTGGTTTCCGGACGACATATCCGCGCGCGGCCTGGAGGAGGAGACTTCGAGG CTGCAGGCCCCACTGGTTGAAAGGAAACTTGGCGCTCCCACCGCCCGGCAGCCCGGGTAC TAGGACCTGNGTAACTGCCCGGGTCCGAATCNACTCAGCGTCCTCCACGCGACCCTCAGC ATGGTGACCTCTACCCCCCGCGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_031280 |
| Insert Size | 900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031280.2, NP_112570.2 |
| RefSeq Size | 891 bp |
| RefSeq ORF | 774 bp |
| Locus ID | 64960 |
| UniProt ID | P82914 |
| Domains | Ribosomal_S15 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207694 | MRPS15 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S15 (MRPS15), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
|
| RC207694L3 | Lenti ORF clone of Human mitochondrial ribosomal protein S15 (MRPS15), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207694L4 | Lenti ORF clone of Human mitochondrial ribosomal protein S15 (MRPS15), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG207694 | MRPS15 (tGFP-tagged) - Human mitochondrial ribosomal protein S15 (MRPS15), nuclear gene encoding mitochondrial protein |
CNY 4,000.00 |
