BHMT2 (AL832258) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832258, the custom clone sequence may differ by one or more nucleotides GGAACACTGGCCAAAGGTATGTAAGATCATTTTCCCCCTCTCTTTCCCTCCCCGTTTTTCCCTTTTGTTC CCTTCTCTTGGTATATAGGTCGTCCTCCAAAATACAATGCAGTGCTGGGGTTTGGAGCCCTTACCCCAAC ATCCCCCCAATCCAGTCATCCTGACTCCCCTGAAAATGAAAAGACAGAGACCACATTCACTTTCCCTGCA CCTGTTCAGCCTGTGTCCCTGCCCAGCCCCACCTCCACAGACGTAAGTAACTTGGGAAAAGCACACTGTC TTTCTGAGAGCTTAAATTGGGAAAAGCGTTATCATATGTTGTCTCCTTATCGACTTGCACCTTCTTGACT AACCCTGGAACAGCAATTAAACACTATAGAATGCTTTTTATGCCATGTAAGGCAGCTTTCTTCTGAGTTT CCAGGCCTTCCATGTCCTTGAGAAACATGTTAAATATAACCTCATGGAACTCAGCCAGCCTTCAGAGTCT GAATTTGATCTACTGTGAATGAGTTCTCAGAAAACAGATGGGGTGGGGAGATCAGCTCATACAGTTGAGA GGTGTGCAGGTTTCTATCATCAGTGTTTTGGGAGTGGGGAAAATGAACAAGACATACAGTCCCCCAGCAG CCCTTGTTTGCTGCTTCAAATACAATTCCAAAAGTGGAAAGTTTTCTTTAGAGCAACTGTTGATCAGAAA CTGTTGAGCAGAAACTCTGCTCTTTAGAGCAGAGTTGATCAGAAACAGGTTCCGGAAAAACTGTCAAATA CTTAGTAGCCCTTCACCCACAAACTGCAGGAATGTGAACTAGTGCTCTAAATGTTTAAATGAACCTCAAC AAAGCTCAGAATTCACTCAGCAGATACTCACTGAGCGCTACTCTGCTGAGCAAGTGCTATTCTTGAGGCT GGGGATACAGCAGGGAACAAAACCAACTCCCTGTCCTCAAGGAGCTCATCTAAAGAGGCAGATAATAAGA AAGTGAATATATAATGTCAGGTAATGATAAGTGCTATGAAGAAAAATGGGGCCGGGCATGGTGGCTTATG CCTGTAATCCCAACACTTTGGGAGGCCGAGGCAGGCAGATCATCTGAAGTCAGGAGTTCAAGACCAGCCT GGCCAACATGGCGAAACCTGGTCTCTACAAAAACACAAAAATTAGCCGGGCATGGCGTGCTCCTGTAATC CCAGTTACTCTGGAGGCTGAGGCAGGAGAATCCCTGGAACCCAGGAGGCGGAGGTTGCAGTGAGCAGAGA TTGTGCCACTGCACTCCAGCCTGGGTGACAAGAGTGAGACTCCATCTCTAAAAAGAGATCAAAAATGGAA TGGATTTAAGGGCATATAGCATACTGGCAGGAGGCTGATGTTTATGAAGAGTGGTGAAGCCTCGGGAGGA TCTGGGGGAAGAGCCTGCCAGGCAGAGGGAACAGCAGTGCAAAGCCCTGAGGTGGGAGTGTACATGGCAT CCTGAAAGAAAAGCAAGAGGGCCAGGATAGCGGGGGCAGAGCAAGCTGGCCAGGGTGGTGGGAAATGAGG CCTCAGAAGTGGCTGGGCCAGTTGCGCAGGGCTTTGTGGCTTTCGTTCTCAGTAAAATGGGAGACCATTA CAGGGTTTTGGGCAGAGGAGTGACATAATTTTTCGGTATTTATTTTGAAAATTTTCAAACCTACAGAAAA GTTACAATAATGGTACAAAGAACCAATGTATATCCTTCACATTGATTCATCAGTTGTTAACATTTTGCCT ATGCTTCTCACCTACCATACACACTTATTTTAGGGGAACAAGGTAACAAGAAGTTGCAGACTTCATGAAT CTTCACCCTAAAATATTTTGCTATATATCTCATAAGAGCAAAGATACTGTCTTATGTAGGTACAATGCAT TTATCAAATTGAGGAAATTTAACACTAATAAAATATTACTATATAAAATATACAGTCCATATTCAGATTG CTCCATTTGTCCCTTACTTACTTTTGTGACACTTTGTTTTTCCTGCTTCAGGGTCCAATTCAAGATTACT CTTGACATTTATTGTCATGATATTGTCTGTTTGGTAATCCTTTATTTTGGAAGAGTTTCTCAGTCTTTGT CTTTCATAACATTGACGTTTTTAAAAAGATAGCCAGTTCTTCTGTAGAATGCCTCTCCGTTTGGATTGTC TGATTTTTTTTCTCAGCAGTAGATTCAGGGTATGCATTTTTGCAGGAGCACTAATAAGTGACGATGGGTC ATTATCAGGGCATCACAGCAGGGGGCACGTGTTGTCAGTCTCTTCTGTTGTTGGTGATGTTAACTTTCAT CTCCTAAGATAGTTTCTATCAGATTTTTCCACCTGTAAATGTGCCTTTTTCCTGTGGTGGAGATAGCTTT GAGTCTGTATGAATATCCAGTTTCCCAACAAACTGTTATCTAATGATAGCATCCTTTAATGATTCTTGCT TGCATTTATTATTTTTAAGATGTATGCAAAATCAACTTGGATTTTAAAAAGGATCACTGTGTCGAAAATA TGCAGAAGAAAAGGGCCTATAGGGAAAATAGGATGGAAGCAGGAAGACCAGTTAGGAGGCTTTTGCAGTT GTCCAGGCAAGAGATGATGATGGGCAGGCACGGTGGCTCACGCCTGTAAATCCCAACACGTTGGGAGGCT GAGGTGGGTGGATTGCTTGAGCCCAGGAGTTCAAGACCAGCCTGGACAACATGGCAAGACCCCTGTCTCT ACAAAACATTAAAAAATTAGCCAGGTGTGATGGCAATATAGTGAAACCCCGTCTCTACTAAATATACAAA AATTAGCCGGGCATACTGGTGTGCCCCTTTAGTCACAGCTGCTTGCAAGGCTGAGGTGGGAGGATCACTT GAGCATGGGAGGCTGAGGCTGCAGTGAGCCATGATTGCGCCACTGCACTCCGGCCTGGGTGACAGAACAA GACCCTGTCTCAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832258 unedited GTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTATGAAGAGTGGTGA AGCCTCGGGAGGATCTGGGGGAAGAGCCTGCCAGGCAGAGGGAACAGCAGTGCAAAGCCC TGAGGTGGGAGTGTACATGGCATCCTGAAAGAAAAGCAAGAGGGCCAGGATAGCGGGGGC AGAGCAAGCTGGCCAGGGTGGTGGGAAATGAGGCCTCANAAGTGGCTGGGCCAGTTGCGC AGGGCTTTGTGGCTTTCGTTCTCAGTAAAATGGGAGACCATTACAGGGTTTTGGGCAGAG GAGTGACATAATTTTTCGGTATTTATTTTGAAAATTTTCAAACCTACAGAAAAGTTACAA TAATGGTACAAAGAACCAATGTATATCCTTCACATTGATTCATCAGTTGTTAACATTTTG CCTATGCTTCTCACCTACCATACACACTTATTTTAGGGGAACAAGGTAACAAGAAGTTGC AGACTTCATGAATCTTCACCCTAAAATATTTTGCTATATATCTCATAAGAGCAAAGATAC TGTCTTATGTAGGTACAATGCATTTATCAAATTGAGGAAATTTAACACTAATAAAATATT ACTATATAAAATATACAGTCCATATTCAGATTGCTCCATTTGTCCCTTACTTACTTTTGN GACACTTTGNTTTTCCTGCTTCAGGNTCCAATTCAAGATTACTCTTGACATTTATTGNCA TGATAATTGNCTGGTTGGGAATCCTTTATTNTGGAAGAGTTCTCAGTCTTTGGCTTCACT ACATTGACGTTTTTAAAAGATGCCAGGNTCTCTGTANAGGCCTCTCGTTTGGATGGCTGA TTTTTTTCTACAGAGATTCAGGTATGCATTTTGCCGGACACTATTATGACGAGGGTCTTT TCAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL832258 unedited NGATATACTATGNACCGCGCCGCAANCTANNGATCGATTTTTTTTTTTTTTTTTTGAGAC AGGGTCTTGTTCTGTCACCCAGGCCGGGGTGCAGTGGCGCAATCATGGCTCACTGCAGCC TCAGCCTCCCATGCTCAAGTGATCCTCCCACCTCAGCCTTGCAAGCAGCTGTGACTAAAG GGGCACACCAGTATGCCCGGCTAATTTTTGTATATTTAGTAGAGACGGGGTTTCACTATA TTGCCATCACACCTGGCTAATTTTTTAATGTTTTGTAGAGACAGGGGTCTTGCCATGTTG TCCAGGCTGGTCTTGAACTCCTGGGCTCAAGCAATCCACCCACCTCAGCCTCCCAACGTG TTGGGATTTACAGGCGTGAGCCACCGTGCCTGCCCATCATCATCTCTTGCCTGGACAACT GCAAAAGCCTCCTAACTGGTCTTCCTGCTTCCATCCTATTTTCCCTATAGGCCCTTTTCT TCTGCATATTTTCGACACAGTGATCCTTTTTAAAATCCAAGTTGATTTTGCATACATCTT AAAAATAATAAATGCAAGCCAGAATCATTAAAGGATGCTATCATTAAATAACAGTTTGGT TGGGAACTGGATAATCATACAGACTCAAAGCTATCTCCACCCCAGGAAAAAGGCCATTTA CAGGTGGAAAAATCTGATAAAAACTATCCTAAGGAGAATAAAGGTAACATCCCCCACCAC CGAAGAAAACTGACAACCCGTGCCCCCCCTGTTTGGAGGCCCTGATAAAGAACCATCGCC ATCTATTAATGGCTCCCGCAAAAATGCCTCCCCCGGAATCTACTGGTGGGAAAAAAAAAA ACGAACAATCCCAACACGAAAGGCCATTCTACACGAAAAAACTGGCCTATTTTTTTTTAA AACACACCTAAAGGTTATAAAG
Restriction Sites	NotI-NotI
ACCN	AL832258
Insert Size	1600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AL832258.1
RefSeq Size	2972 bp
RefSeq ORF	2972 bp
Locus ID	23743
Gene Summary	Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

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