OSTM1 (BC016376) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GIPN; GL; HSPC019; OPTB5
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC016376, the custom clone sequence may differ by one or more nucleotides GGGGGCGGAAACCGGAAGCGGCGGCTGTCCGCGGTGCCGGCTGGGGGCGGAGAGGCGGCGGTGGGCTCCC TGGGGTGTGTGAGCCCGGTGATGGAGCCGGGCCCGACAGCCGCGCAGCGGAGGTGTTCGTTGCCGCCGTG GCTGCCGCTGGGGCTGCTGCTGTGGTCGGGGCTGGCCCTGGGCGCGCTCCCCTTCGGCAGCAGTCCGCAC AGGGTCTTCCACGACCTCCTGTCGGAGCAGCAGTTGCTGGAGGTGGAGGACTTGTCCCTGTCCCTCCTGC AGGGTGGAGGGCTGGGGCCTCTGTCGCTGCCCCCGGACCTGCCGGATCTGGATCCTGAGTGCCGGGAGCT CCTGCTGGACTTCGCCAACAGCAGCGCAGAGCTGACAGGGTGTCTGGTGCGCAGCGCCCGGCCCGTGCGC CTCTGTCAGACCTGCTACCCCCTCTTCCAACAGGTCGTCAGCAAGATGGACAACATCAGCCGAGCCGCGG GGAATACTTCAGAGAGTCAGAGTTGTGCCAGAAGTCTCTTAATGGCAGATAGAATGCAAATAGTTGTGAT TCTCTCAGAATTTTTTAATACCACATGGCAGGAGGCAAATTGTGCAAATTGTTTAACAAACAACAGTGAA GAATTATCAAACAGCACAGTATATTTCCTTAATCTATTTAATCACACCCTGACCTGCTTTGAACATAACC TTCAGGGGAATGCACATAGTCTTTTACAGACAAAAAAATTATTCAGAAGTATGCAAAAACTGCCGTGAAG CATACAAAACTCTGAGTAGTCTGTACAGTGAAATGCAAAAAATGAATGAACTTGAGAATAAGGCTGAACC TGGAACACATTTATGCATTGATGTGGAAGATGCAATGAACATCACTCGAAAACTATGGAGTCGAACTTTC AACTGTTCAGTCCCTTGCAGTGACACAGTGCCTGTAATTGCTGTTTCTGTGTTCATTCTCTTTCTACCTG TTGTCTTCTACCTTAGTAGCTTTCTTCACTCAGAGCAAAAGAAACGCAAACTCATTCTGCCCAAACGTCT CAAGTCCAGTACCAGTTTTGCAAATATTCAGGAAAATTCAAACTGAGACCTACAAAATGGAGAATTGACA TATCACGTGAATGAATGGTGGAAGACACAACTTGGTTTCAGAAAGAAGATAAACTGTGATTTGACAAGTC AAGCTCTTAAGAAATACAAGGACTTCAGATCCATTTTTAAATAAGAATTTTCGATTTTTCTTTCCTTTTC CACTTCTTTCTAACAGATTTGGATATTTTTAATTTCCAGGCATAGCAATGTTATCTATTTTAATGTGTAT TTGTCACAATAACAGAACATGCAAGAACAATCATTATTTTATTTTATAGGCATTTGATTACTATTCTAGA CTTCTGGTATCTTCTTACTAACATAAATATCTCAAGTAGAAAAGTTTTTGAAAACTAACATTTAAAAATT AATCAGTTACAGTAAAGACTTTGAAAAAGAAATGTACTTGTTAGGAAGTAGCTTAATTACCCCCCATTGC AGTATTATTGTTATATATATAGTTAATATGTTGTACATCACAATAATATATAATTCAGTCTCTAGTTTCC CTAGAGTCATTTTTGAAACCACTGATTGCAAACCTCCCTGACAATTTTTAAAAGTAGTAAGCCACATTAC ATTTATCTTTGTAAAAAGATTTATGGTAACTGGTTTCTTACTTGACTTTTATAAATAGTATTTTACATCT TATTTTTGCCTTTATTTCATAAGTAATTTAAAAATCACTGGATTGCTTTATTATATTCAGGGCAATATGG ATTATTTTTATACCAAGGATTTGCATCGTGAATTACATTAAGTTATTTGGCAATTTATAATTTATTACTA CTTTAAATCAAATGTAGCATTATCACACTGTATTTAAATTGTCATTTTTTAAAGGAATATTTTCTTCTTA AGATATATAGAGGATTTTGGAGAAGAGAGACAGGAGGGGTAAAACCAGCTTAAGGTTCAGCGAGCAGAAA GGGACCTGAGAGGATGCTCACTGTAAGACTGTTGGACAGTGGTGTGTATTGAGGGGATGAATCGGAACGA TAGTCTCATGCAGAAAATAGTGAGATTAAGATCATCCTTATTGTTTCTAAATTATTTCAATCAGATGAAA GTGATACGATTGAAATGAAATCACATAGTTCGTGCTCAGAAATTCTATTTTGGTATGTTTGTATTAGCCT TTAGAAAAAACACTCCGTTTCAGAATTGTTCACAGTTTTATTTCTTAGGTTTTTAGAGTTCAGGATTTCA TTTATTAATTTCTTCTTGCTTTTTTGGTGGAAATAGGCTTTGTTGTAAACATTAAGAATATAAAATCTCC TCTATATAGAAACAAGAATTTTGTTAAAAAGAGAATTTGAATCCCTTCCTATACTATAAAATGCTCTATA GCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC016376 unedited TTCATACCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA AGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCG CGAATTCGGCACCAGCTCGGCCTGCGCTCGCGGAAACCGGAAGCGGCGGCTGTCCGCGGT GCCGGCTGGGGGCGGAGAGGCGGCGGTGGGCTCCCTGGGGTGTGTGAGCCCGGTGATGGA GCCGGGCCCGACAGCCGCGCAGCGGAGGTGTTCGTTGCCGCCGTGGCTGCCGCTGGGGCT GCTGCTGTGGTCGGGGCTGGCCCTGGGCGCGCTCCCCTTCGGCAGCAGTCCGCACAGGGT CTTCCACGACCTCCTGTCGGAGCAGCAGTTGCTGGAGGTGGAGGACTTGTCCCTGTCCCT CCTGCAGGGTGGAGGGCTGGGGCCTCTGTCGCTGCCCCCGGACCTGCCGGATCTGGATCC TGAGTGCCGGGAGCTCCTGCTGGACTTCGCCAACAGCAGCGCAGAGCTGACAGGGTGTCT GGTGCGCAGCGCCCGGCCCGTGCGCCTCTGTCAGACCTGCTACCCCCTCTTCCAACAGGT CGTCAGCAAGATGGACAACATCAGCCGAGCCGCGGGGAATACTTCAGAGAGTCAGAGTTG TGCCAGAAGTCTCTTAATGGCAGATAGAATGCAAATAGTTGTGATTCTCTCAGAATTTTT TATACCACATGGCANGAGGCAAATTGTGCAAATTGTTTAAACAAACACAGTGAAGAATTA TCAAACAGCACAGTATATTTCCTTAATCTATTTATCACACCCTGACCTGCTTTTGACATA CCCTNCAGGGGAATGCACATAGTCTTTTACAGACAAAAATTATTCAGAGTTTGCAAAACT GCCGTGAAGCATACAAACTCTGAGTAGTCTG
Restriction Sites	NotI-NotI
ACCN	BC016376
Insert Size	3800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC016376.1
RefSeq Size	2483 bp
RefSeq ORF	2483 bp
Locus ID	28962
Protein Families	Transmembrane
Gene Summary	This gene encodes a protein that may be involved in the degradation of G proteins via the ubiquitin-dependent proteasome pathway. The encoded protein binds to members of subfamily A of the regulator of the G-protein signaling (RGS) family through an N-terminal leucine-rich region. This protein also has a central RING finger-like domain and E3 ubiquitin ligase activity. This protein is highly conserved from flies to humans. Defects in this gene may cause the autosomal recessive, infantile malignant form of osteopetrosis. [provided by RefSeq, Jul 2008]

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