USP36 (AK023077) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DUB1
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK023077, the custom clone sequence may differ by one or more nucleotides ATGCTATTGAAGACAGCAGACAGGCCCGGACTGAGACCGTGGTTGATGACTGGGACGAAGAGTTTGACCG AGGGAAGGAAAAGAAAATTAAAAAATTTAAGAGAGAGAAGAGGAGAAACTTCAACGCCTTCCAGAAACTT CAGACTCGACGGAACTTCTGGTCTGTGACTCACCCAGCAAAGGCTGCCAGCCTCAGCTATCGCCGCTGAC TGTGCCCCTGTGGAAGGAGGACTACCGTGGTTTGTTCTGCAGCCTCCTGGAGACAAGGCGTCCCTTCCCG GGAGCTGTCGGTCTGGATCTGAGGGAGCTCTCTGTGTGGGCTCTGCTGTGCTGGGAGCCTGTCACGGTAG GAGCTCTCCCGGTACCAGTGTCCACAGACCGCCCAACATAGAGGCTTTGAGGCTTCTCTAGATCGGAACC TCTTTGGTGACATTCCCGACCAGCCCTGCAAGAGAAACGACAGTGTGTGTGTGAGCAGAGGTGGCTGCAC ACCTGCTGGACATCTTTGCCAGGCTGTGCCTTCTCATGTTTCATAGACAGTGGTCTGTGCTGGCAGAGGC TGCTGCCCCTGGTTGGGGCTATCAGGAGAGTGGGGGATGGTGGCCACATGTCCTCCAGGTGGTCTCCCGG TGCATAGCTGGTGGCTCTGGGCAAGCCATCCCTTGCTTCTCGGGGCTGACGCCACCGTTGTGTCCGAGCC CGCCCTCCCCTGCTTCCTCAGCGGGACCCCTTCATCTGTTGGCCTTACCTGTCCTCAGAAAGGAAGAGGT GACCCCACCCAGCCACCTCTCCCTTTTATGGAACTCGAGAGGGTGGCCCTACTGTGCACCCCTTCCTTGT GAGTAGCTCTCAACTGTCCTGGAGAGCAGAGGCTATTTGGGGTCGGAGGAGCCCTCGATACCTGCGAATA CATCTGCTTTCCAGGCTGCTGTTTATTCTGAGACGACTGTGCTGTAGCTTCCCTTGCAGCTGCAATAACC CGCAGGTCTTCACTGAGGTGGAGGCTTTGGGGTAGAATTCTCCATTTATTTTACTACTTAATACAAAACA TTTATTTTTGACCAGTCCTGTGGCTTCCATTAGCAATATGTTTCCTTTCCCAAATATGCAAATAGTGGCT TTGTTTGCTCAATTTTGTGAGTGCTTTGGAATTTAAATGATTGTATAACTCAAGAAGATTACTTTTCTAT GTTGCTCAAGCTGTGCCTACCAACTTGTAACTTAATAAATACAGGAAATCCTCAGAGAAGGTGATATTTT CAGGAAAAAGACAAATGCCCTCATAGTAGTGGGAAGTGTGAAGGTGACCGTGAACATCCTTCCTCATCGG GTCTGTCCCCGTCATTTCCTCCCGGAGTCGTCGCAGGTGGAGATGGACAACGTGGTGTTGGACTTAGACC TCCTTCAGTGTGGCTCTGCTGGGCCAGAGGCATCCTGCTGTCCCGGGTGGCTGCCTCGCTGTCTGCACCC CCTCTCCCTGGGGCAGCTTTGCTTCCTGCCCCTGTGCTCGGGGCCTGGGTGGTTACTGGCGTGTAGATGG AATTGCTTTTTTAATATGGGAAGATACATTTATTTTTTTCCATGTGGGTGGGTGTCTCTTTTTGGATTTT CTTCTGTTTTTACGTTTCTCTTCTTAGAAGGGTGGGAGAGAATCAAGCTCCTGTGGCCACCTGTGTCCCA GCAGCAGTGAGTGGAGCTGCTCAGGGTGCCCTCTCCTGCGGACCAGTCTCTGAATGTTCAAAGATGAGGG CCTGGCTTCCGTGCTCTGGCTTTGTAACTTATCTGGAAGGGAAAGCACATGCCTTCACGGGCAGGGTATG TTCCTTTTCTTCTCGGGGTGTTGACTTGCATTCCTGTGTGAACTGTTCCCTCTGCCATGTTTACCGTGTG ATGTTCTGTAGTTGAAAATGTTAGTTGTCTGCTGGCACAGAATTTATCTCGTTCCTTTCTCTCCCTTCTC TCCTCCAAATCAGTCTCTTCCCTTCTCCACTAGATAACTGTAAAACCTTTTCCTGGGGTACATACATTCG TTAATTCTTGGGCAGTGGTGAGCACGAGATGACTTTCTGCAGCGTTTATCACTGTTGGGTGGAGTCACGT CCCTTCCCTCCACCGAAGTCATCAACCAGATAGGGAAGGGAAAGATGAGGCCCAGAAAACGAGTTCAAAC TCTAGGTCTTGTACACGTATGTAAGTAAATGTCAATAACCCAAGCCTTTGTCATAGCAGTCACTTGGTTG ACTTAGGATCTGGGTCTGTTGAATTTTGTGCTTGGGAATGGAGCTGGAGGGAGTGGGGCCTGTGTACAGC AGCTACCTCTCCCAGGTCCTCTCACTTGCCTGCCCCGCGTCCTGGTTGCATGGCCGCACCTGTGTGTGTG CAGAGGTCTGTGTCCCATCCTCTGCACCTCCTTTCCGGGGGCCTGGGGAGCCCCACGTGTTGCCAAGATC TTGGTGCAATAAAATACTCCGGTTCTGTG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK023077 unedited TCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA TTCGGCACGAGGCGGCGGTCAGTCAGGATGCTATTGAAGACAGCAGACAGGCCCGGACTG AGACCGTGGTTGATGACTGGGACGAAGAGTTTGACCGAGGGAAGGAAAAGAAAATTAAAA AATTTAAGAGAGAGAAGAGGAGAAACTTCAACGCCTTCCAGAAACTTCAGACTCGACGGA ACTTCTGGTCTGTGACTCACCCAGCAAAGGCTGCCAGCCTCAGCTATCGCCGCTGACTGT GCCCCTGTGGAAGGAGGACTACCGTGGTTTGTTCTGCAGCCTCCTGGAGACAAGGCGTCC CTTCCCGGGAGCTGTCGGTCTGGATCTGAGGGAGCTCTCTGTGTGGGCTCTGCTGTGCTG GGAGCCTGTCACGGTAGGAGCTCTCCCGGTACCAGTGTCCACAGACCGCCCAACATAGAG GCTTTGAGGCTTCTCTAGATCGGAACCTCTTTGGTGACATTCCCGACCAGCCCTGCAAGA GAAACGACAGTGTGTGTGTGAGCAGAGGTGGCTGCACACCTGCTGGACATCTTTGCCAGG CTGTGCCTTCTCATGTTTCATAGACAGTGGTCTGTGCTGGCAGAGGCTGCTGCCCCTGGT TGGGGCTATCAGGAGAGTGGGGGATGGTGGCCACATGTCCNTCCAGTGGTCTCCCGGTGC ATAGCTGGTGGCTCTGGGCAAGCCATCCCTTGCTTCTCGGGGCTGACGCACCGTTGTGTC CGAGCCCGCCCCTCCCTGCTTNNCTCAGCGGACCCCTTCATCTGNTGGNCCCTACCTGTC CTCAGAAAGGAAGAAGTGACCCCCACCCGCCACCTCTCCCTTTNTGGAACTCGAGAGGGT GGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK023077 unedited ATTTAGTTTTTATATTTTTTTTTTTACAAAACCGGGATATTTTATTGCACCAATATCTTG GCAACACGTGGGGCTCCCCAGGCCCCCGGAAAGAGGTGCAGAGGATGGGACACAGACCTC TGCACACACACAGGTGCGGCCATGCAACCAGGACGCGGGGCAGGCAAGTGAGAGGACCTG GGAGAGGTAGCTGCTGTACACAGGCCCCACTCCCTCCAGCTCCATTCCCAAGCACAAAAT TCAACAGACCCAGATCCTAAGTCAACCAAGTGACTGCTATGACAAAGGCTTGGGTTATTG ACATTTACTTACATACGTGTACAAGACCTAGAGTTTGAACTCGTTTTCTGGGCCTCATCT TTCCCTTCCCTATCTGGTTGATGACTTCGGTGGAGGGAAGGGACGTGACTCCACCCAACA GTGATAAACGCTGCAGAAAGTCATCTCGTGCTCACCACTGCCCAAGAATTAACGAATGTA TGTACCCCAGGAAAAGGTTTTACAGTTATCTAGTGGAGAAGGGAAGAGACTGATTTGGAG GAGAGAAGGGAGAGAAAGGAACGAGATAAATTCTGTGCCAGCAGACAACTAACATTTTCA ACTACAGAACATCACACGGTAAACATGGCAGAGGGAACAGTTCACACAGGAATGCAAGTC AACACCCCGAGGAGTAAAGGGACATACCCTGCCCGTGAAGGCATGTGNCTTTCCCTCCCA GATAAGTTACAAAGCCAGAGCACGGGAGCCAGGCCCTCATCTTTGAACATTCAAAGACTG GTCCGCCAGAAAAGGCACCCTGAGCAGCTCCACTCACTGCTGCTGGGGACCCAGTTGGCC C
Restriction Sites	NotI-NotI
ACCN	AK023077
Insert Size	2650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK023077.1
RefSeq Size	2479 bp
RefSeq ORF	2479 bp
Locus ID	57602
Protein Families	Druggable Genome, Protease
Gene Summary	This gene encodes a member of the peptidase C19 or ubiquitin-specific protease family of cysteine proteases. Members of this family remove ubiquitin molecules from polyubiquitinated proteins. The encoded protein may deubiquitinate and stabilize the transcription factor c-Myc, also known as MYC, an important oncoprotein known to be upregulated in most human cancers. The encoded protease may also regulate the activation of autophagy. This gene exhibits elevated expression in some breast and lung cancers. [provided by RefSeq, Mar 2016]

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