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DDIT4L (NM_145244) Human Untagged Clone
CAT＃: SC100121
DDIT4L (untagged)-Human DNA-damage-inducible transcript 4-like (DDIT4L)
CNY 3,600.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | REDD2; Rtp801L |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC100121 sequence for NM_145244 edited (data generated by NextGen Sequencing)
ATGGTTGCAACTGGCAGTTTGAGCAGCAAGAACCCGGCCAGCATTTCAGAATTGCTGGAC TGTGGCTATCACCCAGAGAGCCTGCTAAGTGATTTTGACTACTGGGATTATGTTGTTCCT GAACCCAACCTCAACGAGGTAATATTTGAGGAATCAACTTGCCAGAATTTGGTTAAAATG CTGGAGAACTGTCTGTCCAAATCAAAGCAAACTAAACTTGGTTGCTCAAAGGTCCTTGTC CCTGAGAAACTGACCCAGAGAATTGCTCAAGATGTCCTGCGGCTTTCCTCAACGGAGCCC TGCGGCTTGCGAGGTTGTGTTATGCACGTGAACTTGGAAATTGAAAATGTATGTAAAAAG CTGGATAGGATTGTGTGTGATTCTAGCGTCGTACCTACTTTTGAGCTTACACTTGTGTTT AAGCAGGAGAACTGCTCATGGACTAGCTTCAGGGACTTTTTCTTTAGTAGAGGTCGCTTC TCCTCTGGTTTCAGGAGAACTCTGATCCTCAGCTCAGGATTTCGACTTGTTAAGAAAAAA CTTTACTCACTGATTGGAACAACAGTGATTGAAGGGTCCTAA Clone variation with respect to NM_145244.3 >OriGene 5' read for NM_145244 unedited
CAAATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCAGGGTGGCCG GGGAGGGGTGAGCAGGGTGCCGCTGGCTGCTGGGGTCTGCAGGTCACCGAGTCCCCAGGA GAGGGGACTCCTAAGAAGCCACCTGCCTGTGTTTACCCGGCAGCGAGCGCGCAGGCCCCC GCGAACTCCTGGCAGCGCTCAGGAAAGGCCGTTGCGCCTCGCGAAGGAAACAGAGCCGTT GACCATGGTTGCAACTGGCAGTTTGAGCAGCAAGAACCCGGCCAGCATTTCAGAATTGCT GGACTGTGGCTATCACCCAGAGAGCCTGCTAAGTGATTTTGACTACTGGGATTATGTTGT TCCTGAACCCAACCTCAACGAGGTAATATTTGAGGAATCAACTTGCCAGAATTTGGTTAA AATGCTGGAGAACTGTCTGTCCAAATCAAAGCAAACTAAACTTGGTTGCTCAAAGGTCCT TGTCCCTGAGAAACTGACCCAGAGAATTGCTCAAGATGTCCTGCGGCTTTCCTCAACGGA GCCCTGCGGCTTGCGAGGTTGTGTTATGCACGTGAACTTGGAAATTGATAATTGTATGTA AAAAGCTGGATAGGATTGTGTGTGATTCTAGCGTCGTACCTACTTTTGAGCTTACACTTG TGTTTAAGCAGGAGAACTGCTCATGCACTANCTTCAGGGACTTTTTCTTTAGTAGAGGGC GCTTCTCCTCTGGGTTCAGGAGAACTCTGATCCTCAGCTCAAGATTTCGACTTGTTAAGA AAAAACTTTACTCCCTGATTGGAACCACAGTGATTGCAAGGTCCTAAAAAGGAAAATATA TAAGATATTTTCTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_145244 |
| Insert Size | 3800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145244.2, NP_660287.1 |
| RefSeq Size | 2607 bp |
| RefSeq ORF | 582 bp |
| Locus ID | 115265 |
| UniProt ID | Q96D03 |
| Gene Summary | Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Plasma membrane translocation of REDD1 governed by GPCRs contributes to mTORC1 activation
,Michel, G;Matthes, HW;Hachet-Haas, M;El Baghdadi, K;de Mey, J;Pepperkok, R;Simpson, JC;Galzi, JL;Lecat, S;,
J. Cell. Sci. Dec 2013.
,PubMed ID 24338366
[DDIT4L]
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Other Versions
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|---|---|---|---|
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| RC202324L1 | Lenti ORF clone of Human DNA-damage-inducible transcript 4-like (DDIT4L), Myc-DDK-tagged |
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| RC202324L2 | Lenti ORF clone of Human DNA-damage-inducible transcript 4-like (DDIT4L), mGFP tagged |
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| RC202324L3 | Lenti ORF clone of Human DNA-damage-inducible transcript 4-like (DDIT4L), Myc-DDK-tagged |
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| RC202324L4 | Lenti ORF clone of Human DNA-damage-inducible transcript 4-like (DDIT4L), mGFP tagged |
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| RG202324 | DDIT4L (tGFP-tagged) - Human DNA-damage-inducible transcript 4-like (DDIT4L) |
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| SC322401 | DDIT4L (untagged)-Human DNA-damage-inducible transcript 4-like (DDIT4L) |
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