Crhr1 (NM_001301812) Rat Tagged ORF Clone
CAT#: RR216605
- TrueORF®
Crhr1 (myc-DDK-tagged) - Rat corticotropin releasing hormone receptor 1 (Crhr1), transcript variant 2
"NM_001301812" in other vectors (1)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CRFR1; CRH-R 1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR216605 representing NM_001301812
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACGGCGCCCGCAGCTCCGGCTCGTGAAGGCCCTTCTCCTTCTGGGGCTGAACCCTGTGTCCACCT CCCTTCAGGATCAGCGCTGTGAGAACCTGTCCCTGACCAGCAATGTTTCTGGCCTGCAGTGCAATGCATC CGTGGACCTCATTGGCACCTGCTGGCCCCGGAGCCCTGCGGGCCAGTTGGTGGTCCGACCCTGCCCTGCC TTTTTCTACGGTGTCCGCTACAACACGACAAACAATGGCTACCGGGAGTGCCTGGCCAACGGCAGCTGGG CAGCCCGTGTGAATTATTCTGAGTGCCAGGAGATTCTCAACGAAGAGAAGAGCAAAGTACACTACCATGT TGCAGTCATCATCAACTACCTGGGTCACTGCATCTCCCTGGTAGCCCTCCTGGTGGCCTTTGTCCTCTTC TTGCGGCTCAGGAGCATCCGGTGCCTGAGAAACATCATCCACTGGAACCTCATCTCGGCTTTCATCCTAC GCAACGCCACGTGGTTTGTGGTCCAGCTCACCGTGAGCCCCGAGGTGCACCAGAGCAATGTGGCCTGGTG TAGGTTGGTGACAGCCGCCTACAATTACTTCCATGTAACCAACTTCTTCTGGATGTTCGGTGAGGGCTGC TACCTGCACACAGCCATTGTGCTCACGTACTCCACCGACCGTCTGCGCAAGTGGATGTTCGTCTGCATTG GCTGGGGTGTACCTTTCCCCATCATTGTGGCTTGGGCCATTGGGAAGCTGCACTACGACAATGAAAAGTG CTGGTTTGGCAAACGTCCTGGGGTATACACTGACTACATCTACCAGGGCCCCATGATCCTGGTCCTGCTG ATCAACTTTATCTTTCTCTTCAACATTGTCCGCATCCTCATGACCAAACTCCGGGCATCCACTACATCTG AGACCATTCAGTACAGGAAGGCTGTGAAGGCCACTCTGGTGCTCCTGCCCCTTCTGGGCATCACCTACAT GTTGTTCTTCGTCAACCCTGGAGAGGACGAGGTCTCCAGGGTCGTCTTCATCTACTTCAACTCTTTTCTG GAGTCCTTTCAGGGCTTCTTTGTGTCTGTGTTCTACTGTTTTCTGAACAGTGAGGTCCGCTCCGCTATCC GGAAGAGGTGGCGTCGGTGGCAGGACAAGCACTCCATCAGAGCCCGAGTGGCCCGAGCTATGTCCATCCC CACCTCCCCGACCAGAGTCAGCTTTCACAGCATCAAGCAGTCCACAGCAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR216605 representing NM_001301812
Red=Cloning site Green=Tags(s) MGRRPQLRLVKALLLLGLNPVSTSLQDQRCENLSLTSNVSGLQCNASVDLIGTCWPRSPAGQLVVRPCPA FFYGVRYNTTNNGYRECLANGSWAARVNYSECQEILNEEKSKVHYHVAVIINYLGHCISLVALLVAFVLF LRLRSIRCLRNIIHWNLISAFILRNATWFVVQLTVSPEVHQSNVAWCRLVTAAYNYFHVTNFFWMFGEGC YLHTAIVLTYSTDRLRKWMFVCIGWGVPFPIIVAWAIGKLHYDNEKCWFGKRPGVYTDYIYQGPMILVLL INFIFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSFL ESFQGFFVSVFYCFLNSEVRSAIRKRWRRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001301812 |
ORF Size | 1242 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001301812.1, NP_001288741.1 |
RefSeq Size | 2507 bp |
RefSeq ORF | 1245 bp |
Locus ID | 58959 |
MW | 47.7 kDa |
Gene Summary | This gene encodes a member of the G-protein coupled receptor family. The encoded protein is a receptor for corticotropin-releasing factor (CRH) and urocortin (UCN). The interaction of this protein with CRH and UCN triggers G-protein coupled signaling. This protein plays a pivotal role in mediating the CRH-elicited effects in depression and anxiety. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN216605 | Crhr1 (untagged) - Rat corticotropin releasing hormone receptor 1 (Crhr1), transcript variant 2 |
CNY 3,900.00 |