Tdp1 (NM_001031657) Rat Tagged ORF Clone
CAT#: RR211578
- TrueORF®
Tdp1 (Myc-DDK-tagged ORF) - Rat tyrosyl-DNA phosphodiesterase 1 (Tdp1), (10 ug)
"NM_001031657" in other vectors (3)
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CNY 7,790.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC112732 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR211578 representing NM_001031657
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGGAAAGCAGCTACGGAAAGTGGACAATATCCAGTAGTGACGACAGCGAGGATGAAAAGCCCA AACCTGTCAAACCATCAACATCCTCTGGCCCCCAGGCTGGGCAGGGAGTGTCGAAGGAGCCCACGTACAC CTGCTCAGAGGCCCGCAAGGCTGCACACAAGAGACAGATCTCACCTGTGAAATTCAACAACACAGACTCC GTGTTACCCCACAAGAAGCAGAAGATGGATTCCCCGGAAGGTCTGGGCTGGTGTCTGTCCAGCAGCGATG ATGAGCAGCAGCCGGATGTGACACAGCAGGAGCAGCCTAAGGGAGTGCCACCTCAAGAGAAAAAATATGC CCCCTCAGCCAATGTGACTACTGCCCAGAAAGTTGAGGATCGAAGCCCTCCTGACAGCCATAGGGCCCAA AGGGCAGACGAAGAGTACGAGACCTCAGGGGAAGGCCAGGACATCTGGGACATGCTGGATAAGGAGAACC CCTTCCAGTTTTATCTCACTAGAGTCTCGGGGATTAAGGCAAAGTACAACTCCAAAGCCCTCCACATCAA GGATATTTTATCCCCTCTGTTTGGGACACTTGTCTCTTCAGCTCAGTTTAACTACTGCTTTGATGTGAAC TGGCTCATAAAGCAATATCCACCAGAATTCAGGAAGAAGCCGATCCTGCTTGTGCACGGCGATAAGAGGG AAGCCAAGGCAGACTTGCATGCCCAGGCGAAGCCCTATGCAAACATTTCCCTCTGCCAGGCAAAGTTGGA TATTGCATTTGGAACACACCACACGAAAATGATGCTCTTGCTCTATGAAGAAGGCCTCCGAGTCGTCATC CACACCTCCAACCTCATCCGGGAAGACTGGCACCAGAAAACCCAAGGAATATGGTTGAGCCCTTTGTACC CACGAATTTACCAGGGAAACCACACATCCGGAGAGTCAAGCACCCATTTCAAAGCGGACCTCACCAGTTA TTTGATGGCGTATAATGCCCCCCCTCTCCAGGAATGGATAGACATCATCCAAGAACACGATCTCTCTGAA ACCAATGTTTACCTTATTGGTTCAACCCCTGGACGCTTTCAAGGAAGTCACAAAGATAATTGGGGACATT TTAGACTTAGAAAGCTTCTGCAGGCGCATGCGCCATCTGCACCCAGGGGCGAGTGCTGGCCCGTCGTGGG TCAGTTTTCAAGCATTGGCTCCCTGGGGCCTGATGAATCCAAGTGGTTGTGCTCAGAGTTTAAAGAGAGC TTGCTGGCAGTGAGAGAGGAAGGCCGGACCCCGGGCAGAAGCGCAGTTCCTCTTCATCTGATCTATCCCT CTGTGGAAAATGTGCGGACCAGCTTAGAAGGATATCCTGCTGGGGGCTCTCTTCCCTATGGCATCCAGAC GGCTGAGAAACAGAGGTGGCTGCACCCCTACTTCCACAAGTGGTCAGCAGAGACATCCGGCCGAAGCAAT GCCATGCCACATATTAAGACGTACATGAGGCCCTCACCAGACTTCAGTAAACTGGCTTGGTTCCTTGTCA CAAGTGCAAATCTGTCCAAGGCTGCCTGGGGAGCACTGGAGAAGAACGGCGCTCAACTGATGATCCGCTC CTATGAGCTGGGGGTCCTTTTCCTCCCGTCGGCCTTTGGCCTGGACACCTTCAAAGTGAAACAGAAATTC TTCTCCAGCAGCAGTGAGCCAATGGCCTCCTTTCCTGTGCCCTATGACCTGCCCCCAGAGCTCTACGGGA GTAAAGATCGGCCATGGATTTGGAACATTCCTTATGTCAAAGCACCAGACACACATGGCAACATGTGGGT GCCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211578 representing NM_001031657
Red=Cloning site Green=Tags(s) MSQESSYGKWTISSSDDSEDEKPKPVKPSTSSGPQAGQGVSKEPTYTCSEARKAAHKRQISPVKFNNTDS VLPHKKQKMDSPEGLGWCLSSSDDEQQPDVTQQEQPKGVPPQEKKYAPSANVTTAQKVEDRSPPDSHRAQ RADEEYETSGEGQDIWDMLDKENPFQFYLTRVSGIKAKYNSKALHIKDILSPLFGTLVSSAQFNYCFDVN WLIKQYPPEFRKKPILLVHGDKREAKADLHAQAKPYANISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVI HTSNLIREDWHQKTQGIWLSPLYPRIYQGNHTSGESSTHFKADLTSYLMAYNAPPLQEWIDIIQEHDLSE TNVYLIGSTPGRFQGSHKDNWGHFRLRKLLQAHAPSAPRGECWPVVGQFSSIGSLGPDESKWLCSEFKES LLAVREEGRTPGRSAVPLHLIYPSVENVRTSLEGYPAGGSLPYGIQTAEKQRWLHPYFHKWSAETSGRSN AMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGAQLMIRSYELGVLFLPSAFGLDTFKVKQKF FSSSSEPMASFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001031657 |
ORF Size | 1827 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001031657.1, NP_001026827.1 |
RefSeq Size | 2096 bp |
RefSeq ORF | 1830 bp |
Locus ID | 314380 |
UniProt ID | Q4G056 |
MW | 68.6 kDa |
Gene Summary | DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN211578 | Tdp1 (untagged ORF) - Rat tyrosyl-DNA phosphodiesterase 1 (Tdp1), (10 ug) |
CNY 7,790.00 |
|
RR211578L3 | Lenti ORF clone of Tdp1 (Myc-DDK-tagged ORF) - Rat tyrosyl-DNA phosphodiesterase 1 (Tdp1), (10 ug) |
CNY 9,690.00 |
|
RR211578L4 | Lenti ORF clone of Tdp1 (mGFP-tagged ORF) - Rat tyrosyl-DNA phosphodiesterase 1 (Tdp1), (10 ug) |
CNY 10,550.00 |