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Limk2 (NM_024135) Rat Tagged ORF Clone
CAT＃: RR207531
- TrueORF®
Limk2 (Myc-DDK-tagged ORF) - Rat LIM domain kinase 2 (Limk2), (10 ug)
"NM_024135" in other vectors (3)
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CNY 8,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Limk2b; Link2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207531 representing NM_024135
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTGGCGGGTGAAGAGGCCTGGAGGTGTCGGGGCTGTGGCAACTATGTTCCCCTGAGCCAGA GGCTGTACAGGACAGCCAACGAGGCCTGGCATAGCTCCTGCTTCCGGTGTTCCGAGTGCCAGGAGTCCCT CACCAACTGGTACTATGAGAAGGACGGGAAGCTCTACTGCCACAAGGACTACTGGGCCAAGTTTGGAGAA TTCTGCCACGGGTGCTCCCTGCTGATGACAGGGCCAGCCATGGTGGCTGGGGAGTTTAAGTACCACCCAG AGTGCTTTGCCTGCATGAGTTGCAAGGTGATCATCGAGGATGGGGATGCGTACGCCCTGGTGCAGCATGC TACACTCTACTGCGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAAAGACTGTCTACGGAG TCTGTCCAGGACCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCTGCCACCACCGAGTGCAGACGTG GCTTCTCTGTGTCTGTGGAAAGCGCCTCCTCTAACTATGCCACCACCGTGCAAGTGAAAGAGGTCAACCG GATGCACATCAGCCCCAACAACCGAAATGCCATCCACCCCGGGGACCGCATCTTGGAGATCAATGGGACC CCTGTCCGCACTCTCCGAGTAGAAGAGGTGGAGGATGCGATTAACCAGACGAGCCAGACACTTCAACTGC TGATTGAACATGACCCTGTCCCCCAGCGCCTGGACCAGTTACGGCTAGACACCCGGCTTTCTCCCCACAT GCAGAGTAGTGGACATACTCTCATGCTCAGCACCTTGGACGCCAAGGAAAATCAGGAGGGAACACTGAGG AGACGTTCTCTGAGGCGCAGTAACAGCATCTCCAAGTCTCCTGGCCCCAGCTCCCCCAAGGAGCCGCTGC TCCTGAGCCGAGACATCAGTCGCTCAGAGTCCCTTCGCTGCTCCAGCAGCTACTCACAGCAGATCTTCCG GCCCTGTGACCTGATCCACGGGGAGGTCCTGGGGAAGGGCTTCTTCGGACAGGCCATCAAGGTGACTCAC AAAGCCACAGGCAAAGTGATGGTCATGAAGGAGTTAATTCGCTGTGATGAGGAAACCCAGAAGACTTTCC TGACTGAGGTGAAAGTGATGCGAAGCCTGGACCACCCTAACGTGCTTAAGTTCATCGGGGTCCTGTACAA GGACAAGAAGCTGAATCTGCTGACAGAGTACATTGAGGGGGGCACCCTGAAGGACTTTCTGCGCAATGTG GACCCGTTTCCCTGGCAACAGAAGGTCAGGTTTGCCAAAGGCATCGCTTCCGGAATGGCTTATTTACACT CCATGTGCATCATCCACCGAGATCTGAACTCGCACAACTGTCTCATCAAATTGGACAAGACGGTGGTGGT AGCAGACTTCGGGCTGTCACGTCTCATAGTGGAAGAGAGGAAAAGGCCTCCAGTAGAGAAGGCCGCCACC AAGAAACGCACCTTACGCAAGAGTGATCGCAAGAAGCGCTACACTGTGGTGGGAAACCCCTACTGGATGG CCCCTGAGATGCTGAATGGCAAGAGCTACGATGAGACTGTGGATGTCTTCTCTTTTGGGATTGTTCTCTG TGAGATCATTGGGCAGGTATATGCTGATCCCGACTGCCTTCCCCGCACACTGGACTTCGGCCTCAACGTA AAGCTTTTCTGGGAGAAGTTTGTCCCAACAGACTGTCCCCCAGCCTTCTTCCCCCTGGCTGCTATCTGCT GCAAACTGGAGCCTGAGAGCAGACCGGCATTCTCAAAGCTGGAGGACTCATTCGAGGCGCTCTCCCTGTT CTTGGGAGAGTTGGCCATCCCACTGCCAGCAGAGCTGGAAGAGCTGGACCACACTGTGAGCATGGAGTAT GGCCTAACCCGGGACTCGCCACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207531 representing NM_024135
Red=Cloning site Green=Tags(s) MAALAGEEAWRCRGCGNYVPLSQRLYRTANEAWHSSCFRCSECQESLTNWYYEKDGKLYCHKDYWAKFGE FCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTE SVQDQLPYSVTLISMPATTECRRGFSVSVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGT PVRTLRVEEVEDAINQTSQTLQLLIEHDPVPQRLDQLRLDTRLSPHMQSSGHTLMLSTLDAKENQEGTLR RRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTH KATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRNV DPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKAAT KKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNV KLFWEKFVPTDCPPAFFPLAAICCKLEPESRPAFSKLEDSFEALSLFLGELAIPLPAELEELDHTVSMEY GLTRDSPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024135 |
| ORF Size | 1914 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024135.2, NP_077049.2 |
| RefSeq Size | 3455 bp |
| RefSeq ORF | 1917 bp |
| Locus ID | 29524 |
| UniProt ID | P53670 |
| MW | 72.2 kDa |
| Gene Summary | kinase with a LIM-kinase (LIMK) domain; human homolog phosphorylates cofilin [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207531 | Limk2 (untagged ORF) - Rat LIM domain kinase 2 (Limk2), (10 ug) |
CNY 8,270.00 |
|
| RR207531L3 | Lenti ORF clone of Limk2 (Myc-DDK-tagged ORF) - Rat LIM domain kinase 2 (Limk2), (10 ug) |
CNY 10,070.00 |
|
| RR207531L4 | Lenti ORF clone of Limk2 (mGFP-tagged ORF) - Rat LIM domain kinase 2 (Limk2), (10 ug) |
CNY 10,930.00 |
