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Pygl (NM_022268) Rat Tagged ORF Clone
CAT＃: RR206321
- TrueORF®
Pygl (Myc-DDK-tagged ORF) - Rat phosphorylase, glycogen, liver (Pygl), (10 ug)
"NM_022268" in other vectors (3)
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CNY 10,830.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206321 representing NM_022268
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAGCCCTTGACCGACCAGGAAAAGCGACGGCAGATCAGCATCCGCGGCATCGTGGGCGTGGAGA ACGTAGCGGAGCTGAAAAAGGGCTTCAATCGTCACCTGCACTTCACTCTGGTCAAGGACCGCAATGTGGC CACCCCCCGCGACTACTACTTCGCCCTTGCGCACACAGTGCGCGACCACCTGGTGGGGCGCTGGATCCGC ACACAGCAGCACTACTATGACAAGTGCCCCAAGAGGGTGTATTACCTCTCTCTGGAATTTTACATGGGCC GAACATTACAGAACACCATGATCAACCTTGGCTTACAGAATGCCTGCGACGAAGCTATTTACCAGCTCGG GCTGGACATGGAGGAGTTGGAAGAAATTGAAGAAGATGCTGGGCTTGGCAATGGTGGTCTTGGGAGGCTT GCTGCCTGCTTCTTGGACTCCATGGCAACGCTGGGGCTTGCAGCCTATGGATACGGCATCCGTTATGAAT ATGGAATCTTCAATCAGAAGATCCGAGAAGGGTGGCAGGTAGAGGAGGCAGATGACTGGCTCAGGCATGG AAACCCTTGGGAGAAGGCTCGTCCTGAATTCATGCTGCCTGTGCATTTCTACGGAAGAGTAGAGCACACC CAGGCAGGAACAAAGTGGGTCGACACCCAGGTGGTGCTGGCTTTGCCGTACGACACCCCCGTACCTGGGT ATATGAACAACACGGTGAACACTATGCGCCTCTGGTCGGCCCGAGCACCCAATGACTTTAACCTTCAAGA CTTTAATGTCGGAGACTACATTCAGGCTGTGCTGGACCGGAACCTGGCTGAGAATATCTCCAGAGTGCTG TACCCCAACGATAACTTTTTTGAAGGGAAGGAGCTGAGGCTGAAGCAGGAGTACTTTGTGGTGGCTGCGA CCCTGCAGGATGTCATCCGACGTTTCAAGGCCTCCAAGTTCGGCTCCAAGGATGGTGTAGGAACCGTGTT TGATGCTTTTCCAGATCAGGTAGCCATCCAGCTGAATGACACACATCCCGCACTCGCCATCCCGGAGCTG ATGAGGATCTTTGTGGACATTGAAAAATTGCCCTGGTCCAAGGCCTGGGAGATCACCAAGAAGACCTTTG CCTACACCAACCACACGGTGCTCCCGGAGGCCCTGGAGCGCTGGCCAGTGGACCTGGTGGAGAAGCTGCT GCCTCGACACTTGCAGATCATTTATGAGATCAATCAGAAGCATTTAGATAGAATCGTGGCCCTGTTTCCT AAAGACATCGACCGCATGCGGCGGATGTCTCTCATCGAAGAGGAAGGAGGCAAAAGGATCAACATGGCCC ACCTCTGCATCGTGGGCTGCCACGCGGTGAACGGGGTAGCGAAGATCCACTCGGACATCGTGAAGACCCA AGTATTCAAGGACTTCAGTGAGCTAGAACCAGACAAGTTCCAGAATAAAACCAACGGGATCACCCCGAGG CGCTGGCTCTTACTCTGCAACCCAGGGCTGGCTGACTTGATAGCAGAGAAAATTGGAGAAGACTATGTGA AAGACCTGAGCCAGCTGACGAAGCTCCACAGCTTCGTGGGCGACGACATCTTCCTCCGGGAAATAGCCAA AGTGAAGCAGGAAAATAAACTGAAATTCTCCCAGTTCCTGGAAAAGGAGTACAAGGTGAAGATCAACCCA TCTTCCATGTTTGACGTGCACGTGAAGCGGATCCACGAGTACAAACGACAGCTTCTGAACTGCCTGCATG TGATCACCATGTACAATCGCATCAAGAAAGACCCTAAGAAGTTCTTCGTGCCAAGGACAGTCATAATTGG TGGGAAAGCTGCCCCAGGATATCACATGGCCAAAATGATCATAAAGCTGGTCACCTCCGTGGCAGAAGTG GTGAACAACGACCCTATGGTTGGCAGCAAGTTGAAAGTCATCTTCTTGGAGAACTACAGAGTGTCTCTTG CTGAAAAAGTCATTCCAGCCACGGACCTGTCAGAACAGATCTCCACTGCTGGCACGGAAGCCTCGGGGAC GGGCAACATGAAGTTCATGCTGAACGGGGCCCTGACCATCGGGACTATGGATGGGGCCAATGTGGAGATG GCGGAGGAGGCCGGGGAGGAAAACCTGTTCATCTTTGGCATGAGGGTAGATGATGTGGCCGCTCTGGACA AGAAAGGGTATGAGGCCAAAGAATATTATGAGGCCCTTCCAGAACTGAAGCTGGTCATTGACCAAATTGA CAATGGCTTCTTTTCTCCCAATCAGCCAGACCTCTTCAAAGACATCATCAACATGTTATTTTATCATGAC AGATTTAAAGTCTTTGCAGACTACGAAGCCTATGTCAAGTGTCAAGAAAAAGTCAGTCAGCTGTATATGA ATCAAAAAGCCTGGAACACAATGGTTCTCAGAAACATAGCTGCCTCGGGGAAGTTCTCCAGTGACCGAAC AATCAGGGAGTATGCCAAGGACATCTGGAACATGGAGCCTTCCGATCTGAAGATCTCCCTATCTAAGGAG TCCAGCAATGGGGTCAACGCCAATGGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206321 representing NM_022268
Red=Cloning site Green=Tags(s) MAKPLTDQEKRRQISIRGIVGVENVAELKKGFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIR TQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDMEELEEIEEDAGLGNGGLGRL AACFLDSMATLGLAAYGYGIRYEYGIFNQKIREGWQVEEADDWLRHGNPWEKARPEFMLPVHFYGRVEHT QAGTKWVDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLQDFNVGDYIQAVLDRNLAENISRVL YPNDNFFEGKELRLKQEYFVVAATLQDVIRRFKASKFGSKDGVGTVFDAFPDQVAIQLNDTHPALAIPEL MRIFVDIEKLPWSKAWEITKKTFAYTNHTVLPEALERWPVDLVEKLLPRHLQIIYEINQKHLDRIVALFP KDIDRMRRMSLIEEEGGKRINMAHLCIVGCHAVNGVAKIHSDIVKTQVFKDFSELEPDKFQNKTNGITPR RWLLLCNPGLADLIAEKIGEDYVKDLSQLTKLHSFVGDDIFLREIAKVKQENKLKFSQFLEKEYKVKINP SSMFDVHVKRIHEYKRQLLNCLHVITMYNRIKKDPKKFFVPRTVIIGGKAAPGYHMAKMIIKLVTSVAEV VNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEM AEEAGEENLFIFGMRVDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPNQPDLFKDIINMLFYHD RFKVFADYEAYVKCQEKVSQLYMNQKAWNTMVLRNIAASGKFSSDRTIREYAKDIWNMEPSDLKISLSKE SSNGVNANGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022268 |
| ORF Size | 2550 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022268.1, NP_071604.1 |
| RefSeq Size | 2715 bp |
| RefSeq ORF | 2553 bp |
| Locus ID | 64035 |
| UniProt ID | P09811 |
| MW | 97.5 kDa |
| Gene Summary | catalyzes the rate-limiting step of glycogen catabolism [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206321 | Pygl (untagged ORF) - Rat phosphorylase, glycogen, liver (Pygl), (10 ug) |
CNY 10,830.00 |
|
| RR206321L3 | Lenti ORF clone of Pygl (Myc-DDK-tagged ORF) - Rat phosphorylase, glycogen, liver (Pygl), (10 ug) |
CNY 12,730.00 |
|
| RR206321L4 | Lenti ORF clone of Pygl (mGFP-tagged ORF) - Rat phosphorylase, glycogen, liver (Pygl), (10 ug) |
CNY 13,870.00 |
