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Akap1 (NM_053665) Rat Tagged ORF Clone
CAT＃: RR204698
- TrueORF®
Akap1 (Myc-DDK-tagged ORF) - Rat A kinase (PRKA) anchor protein 1 (Akap1), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_053665" in other vectors (3)
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CNY 10,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Akap84 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204698 representing NM_053665
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAATCCAGTTCCGCTCACTCTTCCCCTTGGCCTTGCCTGGAATGCTAGCACTCCTTGGCTGGTGGT GGTTTTTCTCTCGTAAAAAAGATCGGCTCAGCAGCAATGGCAAGCAGGTGGGGACATTGAAGGTTGGCCC TGCCATCGAGGACCGACTGCCCACTGAAGAGGCCTGTCCTGGAGTCCTGTCTGTGACCCCCAGTGTCACA CAGCCTCCTGGAAAGGAAGAGCAGCGCTCTATGGACAGGCCTCTTTCAGATCCCCCAGCCTTGCCAAGGA CCCGTCAAGTTCGCCGAAGATCAGAGTCCTCAGGCAACCTCCCCAGCATTGTAGACACAAGGTTGCAGGC AGGACAGTGCAGCGATGAGAATTCAAAAGTGGTACTCTCCCTGATGGGGGACGAAGCCAAATCTATTCCT CTTGGACGTCCGCTTTTCCCAAAGGATCTGTCCTTCCCCTATGAAGCAGTGGAAGGGTGTAAGCAAGAGT CTGCCCTGGGCAGAACTCCTGGGAGAGGCTGGCTAAGCCAGTGTGCAGCCTCTGGAGAGAACGCGAGAGA GACGGGTGGGGCGGAAGGGACTGGAGATGCTGTGTTGGGGGAAAGTGTACTTGAGGAAGGCCTATTGCCC CAGGAGTGTGTTTCAGAAGTAGAGAAGAGTGAGTTTCCGATCCTAGCTCCCGGGGGAGGCGGGGGAGAGA AGGTGAGAAGTGGCCCACCACAGGTAGATGAGCTTTTAAAGAAGGAAGAATATATTGTTGGGAAGTTGCC AAGTAGCTTTGTGGGGCCAGTTCACTCGGAGCTGGTTAAGGATGAGGGTGCGCTGGTACCCCAAGTCAAA GGTAGCCAGGACAGAAGCCTGGCTAGAGAACTGGACAAAGACAAGACCTTGCCTGAAAAGGACCAGATTG AGCAGACTGCCTTCCAGATCATCTCCCAGGTGATCTTGGAAGCGACTGAAGAGATACGGGCCACCACAGT GGGCAAGACTGTGGCACAAGTGCATCCAACCCCGGGCACTCAGCCTCAGGGGCAGGAGGAGAGCTGTGTC CCAGCCAGCCAGGAAACTAGCTTGGGACAAGAAATCCCAGATCCTGCTTCCACCAGAACAGGTGCCACTG CCAGCCCTTCAGCAGGAGCCCCACCACCAAAGACCTATGTGAGCTGTCTCAGCAGTCCTCTGTCAGGCCC CACCAAGGACCAGAAGCCAAAGAACTCTGCACACCACATCTCCCTGGCTCCCTGCCCACCGCCAGTTACC CCCCAGAGGCAGTCTCTGGATGGGGCAAGTAACCCGAGAGGTGATGACACCTTTGTCACCTGTACGTCCA ACAACAGCCAGAGTGTCCTTTCAGTTACCTCCTTGGGGCTGTGCTCAGACCCTGTCAGTACTTCGAGGCT TGAAGACTCTTGCACAGAGACCATCTCAAGCTCCGGAGACAAAGCTGTCACCCCACCACTGCCAGACAGT ACTGAGCCCTTCAGCAATGGGGTGCTGAAGGAGGAGTTGTCAGACTTGGGGACCGAGGATGGATGGACCA TGGATACAGAAGCAGATCATTCAGGAGGTTCTGACGGGAACAGTATGGATTCAGTGGATAGCTGTTGTGG GCTTACGAAACCTGATAGCCCCCAGACTGTCCAGGCAGGCTCCAACCCTAAGAAGGTTGACCTCATCATC TGGGAGATCGAGGTGCCAAAGCATTTAGTTGGTCGACTGATTGGCAAGCAGGGACGGTACGTGAGTTTTC TGAAGCAGACGTCTGGTGCCAAGATCTACATCTCCACCCTGCCTTACACACAGAACATCCAGATCTGCCA CATAGAAGGCTCTCAGCATCATGTGGACAAAGCTCTGAACTTGATTGGGAAGAAGTTTAAGGAGCTGAAC CTCACCAATATCTATGCACCACCGCTGCCTTCTCTGGCACTGCCTTCCCTGCCGATGACATCGTGGCTCA TGCTGCCTGATGGTATCACTGTGGAAGTCATCGTGGTCAACCAGGTCAATGCTGGGCACCTATTTGTACA GCAACACACACACCCCACCTTCCACGCACTGCGCAGCCTGGACCAGCAGATGTACCTCTGTTACTCTCAG CCTGGAATCCCCACCTTGCCCACCCCAGTGGAAATAACGGTTATCTGCGCTGCCCCTGGTGCGGACGGGG CCTGGTGGCGAGCCCAAGTAGTGGCCTCCTATGAGGAGACCAATGAGGTGGAGATTCGCTACGTGGACTA TGGTGGATATAAGAGGGTGAAAGTCGACGTGCTCCGGCAAATTAGGTCTGACTTTGTGACCCTGCCATTC CAAGGAGCAGAAGTCCTTCTGGACAGTGTGGTTCCGCTGTCAGATGATGATCACTTTTCACCCGAGGCAG ACGCAGCCATGAGTGAGATGACAGGCAATACAGCACTGCTGGCCCAGGTGACAAGCTACAGTGCGACTGG CCTTCCTCTGATTCAGCTATGGAGTGTGGTTGGAGATGAAGTGGTGTTGATAAACCGGTCGCTGGTGGAG CGGGGCCTGGCACAGTGGGTAGACAGCTGCTATGCCAGCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204698 representing NM_053665
Red=Cloning site Green=Tags(s) MAIQFRSLFPLALPGMLALLGWWWFFSRKKDRLSSNGKQVGTLKVGPAIEDRLPTEEACPGVLSVTPSVT QPPGKEEQRSMDRPLSDPPALPRTRQVRRRSESSGNLPSIVDTRLQAGQCSDENSKVVLSLMGDEAKSIP LGRPLFPKDLSFPYEAVEGCKQESALGRTPGRGWLSQCAASGENARETGGAEGTGDAVLGESVLEEGLLP QECVSEVEKSEFPILAPGGGGGEKVRSGPPQVDELLKKEEYIVGKLPSSFVGPVHSELVKDEGALVPQVK GSQDRSLARELDKDKTLPEKDQIEQTAFQIISQVILEATEEIRATTVGKTVAQVHPTPGTQPQGQEESCV PASQETSLGQEIPDPASTRTGATASPSAGAPPPKTYVSCLSSPLSGPTKDQKPKNSAHHISLAPCPPPVT PQRQSLDGASNPRGDDTFVTCTSNNSQSVLSVTSLGLCSDPVSTSRLEDSCTETISSSGDKAVTPPLPDS TEPFSNGVLKEELSDLGTEDGWTMDTEADHSGGSDGNSMDSVDSCCGLTKPDSPQTVQAGSNPKKVDLII WEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQNIQICHIEGSQHHVDKALNLIGKKFKELN LTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQ PGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPF QGAEVLLDSVVPLSDDDHFSPEADAAMSEMTGNTALLAQVTSYSATGLPLIQLWSVVGDEVVLINRSLVE RGLAQWVDSCYASL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053665 |
| ORF Size | 2562 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053665.1, NP_446117.1 |
| RefSeq Size | 2621 bp |
| RefSeq ORF | 2565 bp |
| Locus ID | 114124 |
| UniProt ID | O88884 |
| MW | 91.7 kDa |
| Gene Summary | scaffolding protein that immobilizes and concentrates protein kinase A II (PKAII) isoforms at specific intracellular locations [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204698 | Akap1 (untagged ORF) - Rat A kinase (PRKA) anchor protein 1 (Akap1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 10,930.00 |
|
| RR204698L3 | Lenti ORF clone of Akap1 (Myc-DDK-tagged ORF) - Rat A kinase (PRKA) anchor protein 1 (Akap1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 12,730.00 |
|
| RR204698L4 | Lenti ORF clone of Akap1 (mGFP-tagged ORF) - Rat A kinase (PRKA) anchor protein 1 (Akap1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 13,970.00 |
