Pole3 (NM_001007652) Rat Tagged ORF Clone
CAT#: RR202666
- TrueORF®
Pole3 (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed), epsilon 3 (p17 subunit) (Pole3), (10 ug)
"NM_001007652" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC94182; MGC94830 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202666 representing NM_001007652
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGAGGCCTGAGGACCTAAATCTGCCCAACGCCGTCATTACCAGGATCATCAAGGAGGCGCTAC CGGACGGTGTCAACATCTCCAAGGAGGCCCGAAGCGCCATCTCCCGCGCGGCCAGTGTTTTCGTTTTGTA TGCCACATCCTGTGCCAATAACTTTGCAATGAAAGGAAAGCGCAAGACTCTCAATGCCAGTGATGTACTG TCAGCCATGGAAGAGATGGAGTTCCAACGGTTCGTAACGCCATTGAAAGAAGCGCTAGAAGCATACAGGC GGGAGCAGAAAGGCAAGAAGGAAGCCTCAGAGCAAAAGAAGAAGGACAAAGACAAAAAAGATTGCGAAGA GCAGGACAAAAGCAGAGAGGAGGAGGATGAAGATGAAGAAAGACTGGATGAGGAAGAACAGAACGAAGAG GAGGAGGTAGACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202666 representing NM_001007652
Red=Cloning site Green=Tags(s) MAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVL SAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKDCEEQDKSREEEDEDEERLDEEEQNEE EEVDN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001007652 |
ORF Size | 435 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001007652.2, NP_001007653.1 |
RefSeq Size | 2625 bp |
RefSeq ORF | 438 bp |
Locus ID | 298098 |
UniProt ID | Q642A5 |
MW | 16.7 kDa |
Gene Summary | Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202666 | Pole3 (untagged ORF) - Rat polymerase (DNA directed), epsilon 3 (p17 subunit) (Pole3), (10 ug) |
CNY 3,990.00 |
|
RR202666L3 | Lenti ORF clone of Pole3 (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed), epsilon 3 (p17 subunit) (Pole3), (10 ug) |
CNY 6,080.00 |
|
RR202666L4 | Lenti ORF clone of Pole3 (mGFP-tagged ORF) - Rat polymerase (DNA directed), epsilon 3 (p17 subunit) (Pole3), (10 ug) |
CNY 6,650.00 |