Shroom3 (NM_001100889) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1310470; Shrm
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217719 representing NM_001100889 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACCCCCGAGAACTTGGAGGAGCCTAGCGCCACTCCAAACTCCAGCAGGACCCCCAAGGAGAGAT ACGTTTATCTGGAAGCGCTCCTAGAGGGAGGGGCTCCCTGGGGCTTCACCCTGAAAGGTGGCCTGGAGCA TGGAGAACCATTAATTATTTCCAAGATCGAAGAAGGCGGGAAGGCTGACTCCGTGAGCTCCGGACTGCAA ACTGGAGATGAAGTCATACACATCAATGAGGTGGTGCTGAGCAGCTCCAGGAGAGAGGCTGTCTCCCTGG TGAAAGGATCCTACAAGACCCTCCGGCTGGTGGTACGCAGAGACGTGTGTGCAGCCCCGGGCCATGCAGA TCCTGGTACCTCGAATTCCATCAGCTCAGAGCTGCTCACCTGCAGTCCCCAGCACCGGAAAGCAACATGG TCAGGAGGGGTGAAACTTCGGCTAAAGCAGAGGTGCAGTGAACCTGCCGCTCGCCCACACTCGTGGCATA CCACCAAATTTGGAGAGACCCAGGCTGACGTCAGCATGATGCGGATATCTCAGGGCACAGTGGGCCCTCC TTGGCACCAGAGCTGCCATTCCAGCTCTTCTACGAGTGACCTCTCCAACTACGACCATGCTTATCTGAGG CGGAGCCCTGACCGGTGCAGCTCCCAGGGGAGCATGGAAAGCCTGGGTCCCCGTGGCGGGTCCCCACCTT GCCATCTTCTTTCCCCTGCCAAGTCCACGAGCAGCATCGACCAGCTAGGCCACCTTCATAACAAAAGAGA CTCGGCCTACAGCTCCTTCTCCACCAGTTCCAGCATCTTCGAGTACCCGCCCCCGGGTGGCTCTGCCCGA GAACGTTCAGGCTCCCTGGACATGATTCCTGCCCGGGGCGGCCTCCTAGAAGGGATGAGGCAGGCAGACA TTCGCTATGTCAAGACAGTTTACGATACCCGGAGGGGAGTATCTTCAGAGTATGAGGTGAACCCTTCAGC CTTGCTTCTTCAAGGTAGGGATGCCCACACCTCAGCAGACAGTCAGGGCTGTGCTAAATGGCACAGCATT CCTCGGGGCAAGGGAACACCTCCCCTGCCTTGGAGCCAGCAGTGTTCTGGTTCCCCGGAGACTGCGGACA ACCCACCCCATAAGGCTGGCGCACCCATGCCCCCAACTCGCAGTGACAGTTATGTGGCCCTCCGGCATCG TGAGCGTCCCAGCTCCTGGTCCAGCCTTGATCAGAAAAGGTTCTGCCGTCCTCAGACGAACTCTTTGGGC TCCCAGAAAACCCCATTCGTCGAGGAACAGCTACATACCGTCCCAGAGAGGAGCCCGGAGAACAGCCCCC CAGTGAAACCTAAGCATAATTATACCCAGAAGACGCAGCCTGGCCAACCTCTACTGCCCACGGGCATCTA TCCGGTACCTTCTCCAGAGCCTCACTTTGCCCAGGTGCCCCAGCCCTCTGTGAGTGGTAATGGTACAGTC TACCCTGCACTGGTCAAAGAGAGTGGATACACAGCTGCTCAGGGAACTTGTAGCAAGATGGCTGCCTTAG ACGAGAACGGGAATCAAAAAGAAGTGAGCAGGCCTGGGTTTGCCTTCTGTCAGCCCCCAGAACACGACTC GATGACTCCAGTGGAGAAAAAGCCAGAACCCAGGGCCAAATACATCCCCTACAAAGTCCATTTTTCTTCA GTGCCTGAAAATGAAGACTCCTCCCTCAAGAGACACGTCACACCTCCCCACGGCAATAGCCTATACCCCA GCGAGAGAAAGAACGTTCACGGTGGTGGCAGCAGACCGTGTTCTAATCACCACAGTCTCCCGTCCGCTCA AGCCCAGGCCCCGCACGCGGGTGATGATGACAGGAGGCCTTCCAGGCTCTTCCAGCCCTGGGAGGGTGAC TTTCAGGAAGATCACAATGCCAACCTCAGGCAGAAAGTGGAGAGGGAAGGCCAAGGCCAGGGCCTGCCAG GCAACTCTGGTGGGACCAAATCGACCTTCTCACCTCTCCAGAATATTCCAGAAAATCTAAGAAGACAAAG CAGCGTGGACCTAGGAGAAGGCCTGGAGGTTTACCCCGGTGGCAGATCCACCTGTGCCACCACCACCACC AAGGTGGAGGACCCTGGGAGGAAAGCAGGGCCCGACAACAGGGGCTACCTGGACAGGTCTGTTTCCTATC CAAGGCCCGAGGGGAAAATGAACGCTGCAGAATCCTTCCACAGTGCAGACTCCAGGTATGAAGAGCCCCT GGCCGCGCCACCGCAGCAGACATCCGGTATGAGCCAGAGGAGGCTCAGTGCCAGCAGTACCCCTGCCCTG CAGTACAAGAAGCCCCACTGCTCCGTGCTGGAGAAGGTTTCTAAGATAGAGGAGCGAGAGCAAGGGCGCC ACAGACCCACGAGTGTGGGCAGCTCCGCTTACGCCCTCAGCTACAGGCCTGGTAGGACTGGCCCCACTCC TAGTACTTCCAGCAGTGACCTTGATGATCCAAAAGCCAGCAGCTCCCATTTCTCTGAGTCCGCCGCCGAA CACCTGCGAAATGGGGAGCAGAACCCCCCAAACGGGGAGCCCAAACTGGAAGAGGCTTCCAGGCAGCAGT GCAACCAGCTGCTGAGGAGGACCACTGCAGATGGCCGTGGACCTCCTGCCCGAGGCAGCGAGCCCTCAAG GCCCGAGGCCCGCCTGCTCCGCAGCCAGAGCACCTTTCAGCTCTTCAGTGAGGCGGAGAGAGAAGCCTCG TGGTCGGAGGATAGGCCCAGCACCCCCGAGTCACCCCTCCTAGACGCACCCTTCAGCCGCGCCTACAGGA ACAGCATCAAGGATGCCCAGTCCCGTGTCCTGGGGGCCACATCATTTCGTCGCCGGGACCTGGAACCGGG CACCCCCGCGACTTCGAGGCCTTGGCGCCCGCGACCCGCCTCTGCCCACGTGGGCATGCGGAGCCCCGAG GCCTCGGCACCCTTGTCCTCCCCGCACACCCCTCGGGAGAGACACAGCGTGACCCCGGCCACAGGGACCC CGCCTGCACCCCAGGCTGCCCGCAGAGGGGCACGCCGGCGCCTCACAGCCGAGCAGAAGAAGCGTTCCTA CTCGGAGCCGGAGAAGATGAACGAGGTCGGGGTCTCGGAGGAGGCTGAGTCCGTGCACTGCGTCCCACAG AGGCCAGCGCAGCTGCGCTTCTCGGAGAGCACCGTGGCCGACCGGCGACGCCTCTTCGAGCGCGACGGCA AGGCCTGCTCCACGCTCAGCCTGTCTGGCCCTGAACTGAAGCAGTTCCAGCAGAGCGCCCTGGCCGACTA CATCCAGCGCAAGACCGGCAAGCGGCCCACTGGAGCTGCCTGCACGCCGGAACCCGGGCTGCGCGAGCGC GCACAGAGCGTCTACTTTCAGGCGGTCCCCGCGGCGCCCGAAGGCCCCGGCCTGGCCTCCGCCTGCAGCC TGAGTTCGCTACGGGAGCCCGAGGTGCTGCCCTGCAAGGAGCACCCCCATCAGTCCGCCGCAGATGGTCC ACAGACGCCCCGAGATCGCAGCAGCTCCTTTGCCAGCGGACGCCTAGTCGGGGAGCGACGACGCTGGGAC CCTCAGGCCCCCAGGCAGCTGCTCAGTGGAGCTAACTGCGGACCCAGGGGCGTCCAGAGGATGGACAGGA CCTCCAGAGGGCCGCCTTCCTGGGACATGGTGGTCGGGAAGTCAGGGAAGTCCAAGTCAGCCGAGGATCT GCTGGAGCGCTCGGACACACTGACCGTTCCTGTGCATGTGAGGTCCAGGTCGTCTCCCACCTCGGACAAG AAAGGTCAGGATGTGCTCCTCAGAGAAGACAGTGGCTTTGGTTTTGTGAAGGATCCATATTTGGCTGGCC CTGGATCTAGGCCTCTCAGCTACTCGGACAGAGGCCAAAAGGAGCAGGCATTGCCCCTGCACCATCCTGC CACTTGCTGGAATGGCTCAGGCTACAAAGCCACTGTGGTTTCCTCTACTGAATCTTCCGGAGCCCCAGAT CATCTGAAGCAACTTAGAGCACCTTGCCCTAGGCCACTTTCAGCAGGAATGCACGGCCACTTCCCAGATG CCAGAGCCTCCTCCGTGGCCCCCCTCAGCTCCCCTCTGCCATCACCTGTCCCCTCCGGTTACCGCTCACA GCTTGACATGGATCAGCAGATGGGACATCAGCCTCTCCCCTCCCCTGCCTCTGCAGTGACGCAGCCTCCC AGCCCAAGAAGCCATGAGCTAGAGGAGGGAATGTGGAAGAGGGCGTCCCTGCCTCAGCGACCCCCTCGAC CTTGGAAGTGGGCCCACGCAGTCCGAGAGGACGGCCTTGCTGAGGACGCCTCATCGGCGCCTGAGTTTGC AACCCTGAAGCACTATAAAAACCAGCTGAGTCTTCCGAGTTCGTGCAGCACTTCTGACCCAGACACTCCA GGGAGGATCTCTCTCCGAATATCCGAGTCAGCCCTACAAGCTTCCCCGCCACCCCGGGGGGACTATGATG ATGAGGTGTTCGTGAAGGACTTGCACCCCAAGGTTCCTTCAAGCCCCACATTTGAAGCTCTCCCCCCGCC CCCACCACCACCTTCTCCACTGAGCCAGGAGCCTCTAACGTGTGGCTCAGATGACTTTCCTTCACCTCCT CCCCAAGCCATGTGTGAGGCACCGCTGGATGGCGAAGTATCGGAGGAGCCCGGCAGCGGTTCCTGCGGAC TTCCCAGGGTGACAGCCACGAGGGAAGGACAGGTGCCCGGGGCAGGCCATGCAGAAGGAAGTCAGATAAT GGTTGCCATCCCACCCCAAACTTCGGCCGAAGGCTCAGCAGCCGAGTCCAGCACTGCTGCCCGAGTGAGC GCCCAGCCTCCGCTCCACAGTTTTCCTGGCAAGCAGCCCTGTCCCAGCCAGGCCCGAAACCTCACTTACG AACCTGCTGACAGAACTCAGGATCTAGGGAAGAAAATCCATGCTGAGCCCCAGAAGACTTCAGAAGATAT CCGAACGGAGGCTCTCGCCAAGGAAATTGTCCACCAAGACAAGTCTCTGGCGGACATTTTGGACCCGGAC TCCAGAATGAAGACCACGATGGATCTGATGGAGGGTCTGTTCCCCAGGGATGCTGGTGTGCTGAAGGAGA ACGCTGAGAGGAGAGCCGTAGACAGCACCACCAGGCGTGCTGGGTGGGAAGTCGAGACGAGCGACCACAA GGAGGCAGCGGGTGTGCTGGTCAACTGCCCTGCGTACTACAGTGTCTCTGCTGCCAAGGCGGAACTGCTG AACAAAATCAAAGACATGCCGGAGGAGCTGCGGGAGGAGGAGGAGCAGGAGGACGTCAATGAGAAAAAGG CTGAGCTCATTGGAAGCCTCACCCACAAGCTGGAGACCCTGCAGGAAGCGAAGGGGAGTCTGCTCACGGA CATCAGACTGAACAATGCCCTGGGGGAGGAGGTGGAGGCCCTGATCAGTGAGCTCTGCAAACCCAACGAG TTCGACAAGTACAAGATGTTCATAGGCGACTTGGACAAGGTGGTCAACCTGCTGCTCTCCCTGTCTGGAC GCCTCGCCCGAGTAGAGAACGTCCTCAGTGGCCTTGGTGAAGACGCCAGTAAAGAAGAAAGGAGTTCTCT GAATGAGAAAAGGAAGGTTCTGGCTGGCCAGCATGAGGATGCCCGGGAGCTCAAGGAGAACCTGGACCGG AGGGAGCGCGTGGTGCTGGATATCCTGGCCAATTACCTGTCCGCCGAGCAGCTCCAGGACTACCAGCACT TCGTGAAAATGAAGTCCACACTCCTCATCGAGCAGCGGAAGCTGGATGACAAAATAAAGCTGGGCCAGGA GCAGGTCAGGTGTCTGCTGGAGTCGCTGCCCTCAGACTTCAGGCCCAAGGCAGGGGCCATCTCCCTGCCC CCAGCCCTCGCCAGTCATGTGACTCCTGGGGGGGGCCCTGCATTCGGTGGCGTTTTCCCAACGTTGACCT CTCCTCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100889
Insert Size	5961 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100889.1, NP_001094359.1
RefSeq Size	6781 bp
RefSeq ORF	5961 bp
Locus ID	305230

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.